Publication:
Acute effects of 17β-estradiol and ATP on endothelial cells; Effects of genistein and PMA on calcium response

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dc.contributor.authorsBilsel A.S., Moini H., Emerk K.
dc.date.accessioned2022-03-28T14:51:31Z
dc.date.available2022-03-28T14:51:31Z
dc.date.issued2002
dc.description.abstractVarious studies have shown that 17β-estradiol (E2), has acute effects on cardiovascular system in addition to its genomic effect. Acute administration of E2 had been shown to increase intracellular free calcium concentration (Ca+2)i in human umbilical vein endothelial cells (HUVEC). The present study investigates the signalling pathway responsible for Ca+2 response to E2. In the study, the effect of E2 on phosphoinositide turnover was investigated by use of Dowex-1 anion-exchange columns after labeling cells with myo(3H)inositol. Additionally, the effects of tyrosine phosphorylation inhibitor genistein and protein kinase C activator 4β-phorbol-12β-myristate-13-acetate (PMA) on the Ca+2 response to E2 and ATP were investigated and compared in fura-2 loaded HUVEC. The data demonstrates that E2 treatment causes 45% increase in inositol phosphate production in parallel to increases in (Ca+2)i. Genistein and PMA inhibit the Ca+2 response to E2 ∼75%, 49%, while they inhibit the response to ATP ∼62%, 73% respectively. Our data suggests the involvement of PLC in the signaling stimulated by E2 and indicate the involvement of tyrosine phosphorylation and PKC. Differences in the effect of the inhibitors on E2- and ATP-induced responses suggest that there may be differences in the upstream signaling initiated by E2 and ATP, such as different roles for tyrosine phosphorylation.
dc.identifier.issn13007777
dc.identifier.urihttps://hdl.handle.net/11424/255694
dc.language.isoeng
dc.relation.ispartofTurkish Journal of Haematology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject(Ca+2)i
dc.subject17β-Estradiol
dc.subjectATP
dc.subjectGenistein
dc.subjectHUVEC
dc.subjectInositol phosphates
dc.subjectPhospholipase C
dc.subjectPMA
dc.subjectSignal transduction
dc.titleAcute effects of 17β-estradiol and ATP on endothelial cells; Effects of genistein and PMA on calcium response
dc.typearticle
dspace.entity.typePublication
local.import.packageSS36
local.import.sourceScopus
local.indexed.atSCOPUS
oaire.citation.endPage273
oaire.citation.issue2
oaire.citation.startPage265
oaire.citation.titleTurkish Journal of Haematology
oaire.citation.volume19

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