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KERİMOĞLU, OYA

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KERİMOĞLU

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2022 - 20232
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Subject: Basic Pharmaceutics Sciences ×

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Now showing 1 - 2 of 2
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    PublicationOpen Access
    Formulation and evaluation of vascular endothelial growth factor loaded polycaprolactone nanoparticles
    (2022-01-01) KERİMOĞLU, OYA; ÖZER ÖNDER, SETENAY; ALARÇİN, EMİNE; KARSLI, SEHER; KERİMOĞLU O., Ozer-Onder S., ALARÇİN E., KARSLI S.
    In an attempt to increase molecular stability and provide controlled release, vascular endothelial growth factor (VEGF) was encapsulated into polycaprolactone (PCL) nanoparticles. Both VEGF-free and VEGF-loaded PCL nanoparticles were formulated by w/o/w double emulsion of the dichloromethane-water system in the presence of polyvinyl alcohol ( PVA) and rat serum albumin. To achieve the optimal formulation concerning particle size and monodispersity, studies were carried out with different formulation parameters, including PVA concentration, homogenization time and rate. Scanning electron microscopy and dynamic light scattering analysis showed respectively that particles had a spherical shape with a smooth surface and particle size varying between 58.68-751.9 nm. All of the formulations were negatively charged according to zeta potential analysis. In vitro release study was performed in pH 7.4 phosphate-buffered saline at 37 degrees C and released VEGF amount was measured by enzyme-linked immunosorbent assay (ELISA) method. At the end of the 35th day, 10% of total encapsulated VEGF was released with a sustained-release profile, which fitted the Korsmeyer-Peppas kinetic model. The bioactivation of the nanoparticles was evaluated using XTT and ELISA methods. As a result, the released VEGF was biologically active and also VEGF loaded PCL nanoparticles enhanced proliferation of the human umbilical vein endothelial cells in cell culture.
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    PublicationOpen Access
    Evaluation and characterization of orally disintegrating films loaded donepezil hydrochloride for Alzheimer’s disease
    (2023-01-01) KERİMOĞLU, OYA; Özen M. İ., KERİMOĞLU O.
    Nowadays, innovative and more effective drug dosage forms continue to be researched and developed compared to traditional drug dosage forms. The importance of orally disintegrating films (ODFs) in current studies and advancements cannot be overstated. This study aims to create the optimum ODF formulations using pullulan and lycoat with donepezil hydrochloride (donepezil HCl). Plasticizers such as polyethylene glycol 400 (PEG-400) and glycerine were used to form flexible films. In the characterization studies of the films produced by the solvent casting technique: determination of morphology, determination of folding, determination of disintegration time, determination of thickness, determination of swelling, determination of pH, determination of mass uniformity, determination of In vitro release, and determination of content uniformity studies were carried out. According to the results obtained, the non-brittle, smooth-surfaced films were disintegrated in the mouth in an average of 35 seconds. Since the 10th minute, more than 85% of the active pharmaceutical ingredient (API) has been revealed. These values suggested that the pullulan polymer showed better film-forming capacity when used with lycoat polymer. Ultraviolet (UV)-visible spectrum studies were carried out to validate donepezil HCl. Thanks to the ODFs containing donepezil HCl, an effective and safe, innovative clinical use has been achieved in the treatment of diseases such as dementia and Alzheimer, which are characterized by a decrease in daily life activities and atrophy in cognitive abilities.