Person: ŞEN, ALİ
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ŞEN
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ALİ
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Publication Open Access Two Acylated Isoscutellarein Glucosides with Anti-Inflammatory and Antioxidant Activities Isolated from Endemic Stachys Subnuda Montbret & Aucher ex Benth(SLOVENSKO KEMIJSKO DRUSTVO, 2019-12-18) DOĞAN, AHMET; Sen, Ali; Goger, Fatih; Dogan, Ahmet; Bitis, LeylaIn this study, we report anti-inflammatory and antioxidant activities of two acylated isoscutellarein glucosides isolated from ethyl acetate extract of Stachys subnuda aerial part. 4'-O-methylisoscutellarein-7-O-2 ''-O-(6 ''' acetyl-beta-D-allopyranosyl)-beta-D-glucopyranoside (SS1) and isoscutellarein-7-O-2 ''-O-(6 '''-O-acetyl-beta-D allopyranosyl)-beta-D -glucopyranoside (SS2) were isolated as major compounds from ethyl acetate extract (SSEA). Also, 2 hydroxycinnamic acid derivatives, and 5 isoscutellarein glucoside derivatives in the SSEA were identified using LC-MS/MS. SS1 with IC50 values of 2.35 and 1.98 mu g/mL and SS2 with IC50 values 13.94 and 12.76 mu g/mL showed fairly strong antioxidant activity against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) radicals, respectively. SS1 and SS2 inhibited 5-lipoxygenase (5-LOX) activity with IC(50 )values of 47.23 and 41.60 mu g/mL, respectively. The results demonstrated that SS1 and SS2 have significant anti-inflammatory and antioxidant potential. Acylated flavonoid glycosides have been first reported for Stachys subnuda. Also, the activities of SS1 and SS2 have been investigated for the first time in this study.Publication Open Access Protective effects of Rubus tereticaulis leaves ethanol extract on rats with ulcerative colitis and bio-guided isolation of its active compounds: A combined in silico, in vitro and in vivo study(2022-11-01) ŞEN, ALİ; ÖZBEYLİ, DİLEK; ERTAŞ, BÜŞRA; DOĞAN, AHMET; BİTİŞ, LEYLA; Şen A., Özbeyli D., Teralı K., Göger F., Yıldırım A., Ertaş B., Doğan A., Bitiş L., Şener G.The aim of this study was to evaluate the therapeutic effect of active ethanol extract obtained from the leaves of Rubus tereticaulis (RTME) against colitis, and to purify major compounds from this extract by bioassay-directed isolation. Rats with colitis induced via intra-rectal acetic acid administration (5%, v/v) received RTME or sulfasalazine for three consecutive days. On day four, all rats were decapitated, and the colonic tissue samples were collected for macroscopic score, colon weight, reduced glutathione (GSH), myeloperoxidase (MPO), and malondialdehyde (MDA) analyses. The active compounds and chemical composition of RTME were determined by bio-guided isolation and LC-MS/MS, respectively. Compared to the colitis group, the rats treated with RTME displayed significantly lowered macroscopic scores and colon wet weights (p < 0.001). These effects were confirmed biochemically by a decrease in colonic MPO activity (p < 0.001), MDA levels (p < 0.001), and an increase in GSH levels (p < 0.001). Kaempferol-3-O-β-d-glucuronide (RT1) and quercetin-3-O-β-d-glucuronide (RT2) were found to be the major compounds of RTME, as evidenced by in vitro anti-inflammatory and antioxidant activity-guided isolation. Their anti-inflammatory/antioxidant activities were also predicted by docking simulations. Additionally, quinic acid, 5-caffeoylquinic acid, quercetin pentoside, quercetin glucoside, quercetin-3-O-β-d-glucuronide, kaempferol-3-O-β-d-glucuronide, and kaempferol rutinoside were identified in RTME via using LC-MS/MS. RT2, along with other compounds, may be responsible for the observed protective action of RTME against colitis. This study represents the first report on the beneficial effects of RTME in an experimental model of colitis and highlights the potential future use of RTME as a natural alternative to alleviate colitis.Publication Open Access Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson(2019-06-13) DOĞAN, AHMET; Aybeniz YILDIRIM;ALİ ŞEN;AHMET DOĞAN;LEYLA BİTİŞIn this study, various extracts obtained different parts of Tanacetumcilicicum were investigated for in vitro antioxidant and anti-inflammatoryactivity. Antioxidant activity was tested with three methods; namely 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, 2.2′‐azinobis (3‐ethylbenzothiazolin‐6‐sulphonic acid) (ABTS) radical cation scavengingcapacity, and ferric-reducing antioxidant power (FRAP) assays. Total phenolicand flavonoid contents of extracts were determined by Folin-Ciocalteu andaluminum chloride methods, respectively. Also, anti-inflammatory activity ofthese extracts was evaluated by 5-lipoxygenase inhibition assay. Ethyl acetateextract of capitula of T. cilicicum (TCCEA) showed the highest antioxidantactivity with IC50 values of 22.44 and 30.86 μg/mL against DPPH and ABTSradicals, respectively. At the same time, the highest ferric reducing power wasfound in the TCCEA (42.2 mg TE/g extract). The highest total phenolic contentshave been detected in TCCEA and ethyl acetate extract of leaves of T. cilicicum(TCLEA) with value of 174.1 and 175.6 mg GAE/g extract, respectively.Similarly, the highest total flavonoid contents have been detected in TCCEA andTCLEA with values of 26.94 and 30.48 mg QE/g extract, respectively. TCCEAexhibited strong anti-inflammatory activity with IC50 value of 9.44 μg/mL whencompared to standard indomethacine (22.39 μg/mL). These results demonstratethat TCCEA has a significant antioxidant and anti-inflammatory activity. Also,the results show that TCCEA is a good candidate for further bioactivity-guidedfractionation in the search for new active anti-inflammatory and antioxidantcompounds.Publication Open Access The effects of Urtica dioica L. ethanolic extract against urinary calculi in rats(MARMARA UNIV, 2020-03-12) DOĞAN, AHMET; Keles, Rumeysa; Sen, Ali; Ertas, Busra; Kayali, Damla; Eker, Pinar; Sener, Tarik Emre; Dogan, Ahmet; Cetinel, Sule; Sener, GokselNephrolithiasis is common urological problem and stone formation has multiple underlying pathogenetic factors. We investigated the possible preventive and therapeutic effect of Urtica dioica ethanol extract (UD) on ethylene glycol-induced nephrolithiasis model in rats. Sprague-Daw ley rats were divided into lour groups (n = 10). The control group was given normal drinking water for 8 weeks and was administered vehicle by gastric gavage. Stone formation was induced by adding 0.75% ethylene glycol (EG) to their drinking water. UD (700 mg/kg) was given orally lor 8 weeks to the preventive group and I or last 4 weeks to the treatment respectively. At the end of the experiment, urine, blood samples and kidney tissues were obtained. In 24-hour urine samples, calcium and citrate levels were decreased and oxalate levels were increased in EG whereas LID treatment groups reversed these parameters back to control levels. In addition, serum levels of creatinine and urea were increased in EG while LID significantly reduced these parameters. Malondialdehyde, 8-hydroxydeoxyguanosine and tumor necrosis alpha levels, and caspase- 3 and N-acetyl-beta-glucosaminidase activities were elevated in EG group and showed a decrease in LID treated groups. Glutathione level was decreased in EG group, whereas it was increased in UD preventive group. Histological examination showed an improvement in UD treated groups. Our results suggest that UD is effective both in prevention and treatment for kidney stones. The mechanism underlying this effect may be the antioxidant effect of UD and the effect on the concentration of stone-forming components in the urine.Publication Open Access Chemical composition and biological activities of essential oils of Foeniculum vulgare Mill. and Daucus carota L. growing wild in Turkey(MARMARA UNIV, 2021) DOĞAN, AHMET; Servi, Huseyin; Sen, Ali; Servi, Esra Yildirim; Dogan, AhmetThe chemical composition of essential oils of aerial parts of Daucus carota (DEO) and Foeniculum vulgare (FEO) was specified by GC/MS. Antidiabetic, anti-inflammatory, antioxidant, and antibacterial activities of oils were tested by alpha-glucosidase inhibitory, 5-lipoxygenase inhibitory, DPPH/ ABTS radical scavenging, and broth microdilution methods, respectively. Thirty components were identified in DEO (87.7%). Carotol (27.7%), elemicin (18.1%), and limonene (16.0%) were determined as the main compounds in DEO. Seventeen constituents were identified in FEO (100.0%). Estragole (34.0%), limonene (27.1%), and alpha-pinene (19.5%) were found as major components in FEO. Both oils displayed weak antibacterial activity against Gram-negative bacteria. However, the oils were not effective on Grampositive bacteria. DEO with IC50 values of 1359 and 1579 mu g/mL and FEO with IC50 values of 3106 and 1654 mu g/mL showed weak antioxidant activities against DPPH and ABTS radicals, respectively. DEO and FEO exhibited good antilipoxygenase activities with IC50 values of 87.24 and 58.35 mu g/mL, respectively. DEO presented significantly alpha-glucosidase inhibitory activity with an IC50 value of 44.78 mu g/mL while FEO displayed poor activity with an IC50 value of 1%7 mu g/mL. This study is the first report on the 5-lipoxygenase and alpha-glucosidase inhibitory activities of aerial parts of DEO as well as on the alpha-glucosidase inhibitory activity of aerial parts of FEO. These results indicated that DEO, which has a good antidiabetic and anti-inflammatory activity, is rich in oxygenated sesquiterpene and monoterpene. Also, it showed that FEO, which has a significant anti-inflammatory activity, is rich in monoterpene and phenylpropanoid.