Person:
SESAL, NÜZHET CENK

Profile Picture

Email Address

Birth Date

Research Projects

Organizational Units

OrgUnit Logo
Organizational Unit

Job Title

Last Name

SESAL

First Name

NÜZHET CENK

Name

Filters

2013 - 20203
Reset filters

Settings

Author: ÇOBANOĞLU, GÜLŞAH ×

Search Results

Now showing 1 - 3 of 3
  • No Thumbnail Available
    PublicationMetadata only
    Quorum sensing attenuation properties of ethnobotanically valuable lichens against Pseudomonas aeruginosa
    (TAYLOR & FRANCIS LTD, 2020) ÇOBANOĞLU, GÜLŞAH; Gokalsin, Baris; Berber, Didem; Ozyigitoglu, Gulsah Cobanoglu; Yesilada, Erdem; Sesal, Nuzhet Cenk
    Antimicrobial properties of ethnobotanically valuable lichens are well recognized but it is not known how exactly their therapeutic effects on microbial infections occur. Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen and its nosocomial strains are responsible for high mortality and morbidity rates. It coordinates pathogenesis and virulence via four quorum sensing (QS) mechanisms called las, rhl, pqs and iqs. The objective of this study is to investigate and report the utilization of lichens for QS inhibition against P. aeruginosa. The inhibitory potentials of Ramalina farinacea (L.) Ach. and Platismatia glauca (L.) W.L. Culb. & C.F. Culb extracts were tested against QS systems of P. aeruginosa using biosensor strains (lasB-gfp, rhlA-gfp and pqsA-gfp). All tested lichen acetone extracts have shown inhibitory effects on las, rhl and pqs QS systems. The percentages of QS inhibition were detected between 75.21% and 92.42% for las, 50.62% and 64.22% for rhl, 47.61% and 69.35% for pqs systems. The highest inhibition was detected for the extracts of R. farinacea collected from Bursa-Alacam. We concluded that these lichen species have significant anti-QS potentials against P. aeruginosa and they can be further evaluated as alternative biosources in medicine.
  • No Thumbnail Available
    PublicationMetadata only
    Screening of antimicrobial activity and cytotoxic effects of two Cladonia species
    (Verlag der Zeitschrift fur Naturforschung, 2013) ÇOBANOĞLU, GÜLŞAH; Açikgöz B., Karalti I., Ersöz M., Coşkun Z.M., Çobanoǧlu G., Sesal C.
    The present study explores the antimicrobial activity and cytotoxic effects in culture assays of two fruticose soil lichens, Cladonia rangiformis Hoffm. and Cladonia convoluta (Lamkey) Cout., to contribute to possible pharmacological uses of lichens. In vitro antimicrobial activities of methanol and chloroform extracts against two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), two Gram-positive bacteria (Enterococcus faecalis and Staphylococcus aureus), and the yeast Candida albicans were examined using the paper disc method and through determination of minimal inhibitory concentrations (MICs). The data showed the presence of antibiotic substances in the chloroform and the methanol extracts of the lichen species. The chloroform extracts exhibited more significant antimicrobial activity than the methanol extracts. However, a higher antifungal activity was noted in the methanol extract of C. rangiformis. The maximum antimicrobial activity was recorded for the chloroform extract of C. convoluta against E. coli. The cytotoxic effects of the lichen extracts on human breast cancer MCF-7 cells were evaluated by the trypan blue assay yielding IC50 values of ca. 173 and 167 μg/ml for the extracts from C. rangiformis and C. convoluta, respectively. © 2013 Verlag der Zeitschrift für Naturforschung, Tübingen.
  • No Thumbnail Available
    PublicationMetadata only
    In vitro evaluation of cytotoxic, anti-proliferative, anti-oxidant, apoptotic, and anti-microbial activities of Cladonia pocillum
    (C M B ASSOC, 2017) ÇOBANOĞLU, GÜLŞAH; Ersoz, M.; Coskun, Z. M.; Acikgoz, B.; Karalti, I.; Cobanoglu, G.; Sesal, C.
    The aim of this study was to investigate the anti-proliferative, apoptotic, cytotoxic, and anti-oxidant effects of extracts from the lichen Cladonia pocillumon human breast cancer cells (MCF-7), and to characterize the anti-microbial features. MCF-7 cells were treated with methanolic C. pocillum extract for 24h. The cytotoxicity of the extract was tested with MTT. Moreover, its anti-proliferative effects were examined with immunocytochemical method. Apoptosis and biochemical parameters were detected in MCF-7. The methanol and chloroform extracts of the lichen were tested for anti-microbial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans using the disc diffusion method and calculation of minimal inhibitory concentrations. Although BrdU incorporation was not observed in MCF-7 cells treated with methanol extract at a concentration above 0.2 mg/ mL, a significant decrease was observed int he percentage of PCNA immunoreactive cells in groups treated with 0.2, 0.4, 06, and 0.8 mg/mL methanol extracts of C. pocillum (49 +/- 6.3, 44 +/- 5.2, 23 +/- 2.5, 0, respectively) compared to that of control (85 +/- 4.5). The percentage of apoptotic cells significantly increased in groups treated with 0.2, 0.4, 0.6, and 0.8 mg/mL extracts of the C. pocillum (54 +/- 3.5, 76 +/- 2.6, 77 +/- 1.8, 82 +/- 4.2, respectively) compared with that of control group (3.9 +/- 1.5). The half-maximal inhibitory concentration of the methanol extract against MCF-7 cells was 0.802 mg/mL. Although the chloroform extract showed more effective anti-microbial activity overall, the methanol extract showed higher anti-fungal activity. Collectively, the results of our study indicate that C. pocillum extracts have strong anti-microbial and apoptotic effects. This lichen therefore shows potential for development as a natural anti-microbial, anti-oxidant, and apoptotic agent.