Publication: Nifedipine bağlı dişeti büyümesi görülen ve görülmeyen hastaların klinik ve biyokimyasal açıdan karşılaştırılması
Abstract
Nifedipine Bağlı Dişeti Büyümesi Görülen ve Görülmeyen Hastaların Klinik ve Biyokimyasal Açıdan Karşılaştırılması Doktora Öğrencisi: Ece Yetiş Danışman: Prof. Dr. Leyla Kuru Periodontoloji Amaç: Bu kesitsel çalışmada, nifedipine bağlı dişeti büyümesinin klinik özelliklerinin belirlenmesi, proteom analiziyle patogenetik mekanizmasının araştırılması ve nifedipin kullanan dişeti büyümesi görülmeyen bireyler, enflamatuvar dişeti büyümesi görülen bireyler ve sağlıklı bireyler ile karşılaştırılması amaçlandı. Gereç ve yöntem: Nifedipine bağlı dişeti büyümesi görülen (NB grubu) ve görülmeyen (N grubu); enflamatuvar dişeti büyümesi saptanan (E grubu); sistemik ve periodontal açıdan sağlıklı (S grubu) olmak üzere toplam 54 birey çalışma popülasyonunu oluşturdu. Periodontal klinik parametreler kaydedildi. Eksize edilmiş olan dişeti dokularında sıvı kromatografi-kütle spektrometrisi tekniği kullanılarak gerçekleştirilen proteom analizinde tanımlanan proteinlerin web tabanlı bir biyoinformatik kullanılarak fonksiyonel gen ontolojisi analizi yapıldı. Ekspresyonu belirlenen 2 protein, Western blotlama analizi ile doğrulandı. Verilerin istatistiksel analizi yapıldı. Bulgular: Proteom analizinde tüm örneklerde tespit edilen 2154 adet proteinin 1907’si gruplar arası çoklu karşılaştırmada istatistiksel anlamlılık gösterdi (p<0,05). Bu proteinlerin 132’si NB, 367’si NB grubunun büyüme görülmeyen bölgelerinde, 568’i N, 366’sı E grubu ve 474’ü S grubunda yer aldı. Western blotlama ile NB grubunda Keratin type I cytoskeletal-13 (KRT-13), N grubunda Caveolin-1 (Cav-1) proteinlerinin yüksek seviyede varlığı saptandı. Sonuç: Proteom analizi sonucunda NB ve N gruplarının dokularında farklı proteinlerin eksprese edildiği görüldü. Bu proteinlerden KRT-13 ve Cav-1’in nifedipine bağlı dişeti büyümesinde biyobelirteç olma potansiyeli taşıdığı ve patogenetik mekanizmanın aydınlatılmasına katkıda bulunabileceği düşünülmektedir.
Clinical and Biochemical Comparison of Patients With and Without Nifedipine Induced Gingival Overgrowth PhD Student: Ece Yetiş Supervisor: Prof. Dr. Leyla Kuru Department: Periodontology Aim: In this cross-sectional study it was aimed to examine the clinical features of nifedipine induced gingival overgrowth (NIGO), investigate the pathogenetic mechanism of NIGO by proteomic analysis, in comparison with individuals using nifedipine without GO, individuals with inflammatory GO and healthy individuals. Material and Methods: The study population consisted of 54 individuals as follows, patients under nifedipine medication with (NB group) and without (N group) GO, non-overgrown regions of NIGO (NBY group), inflammatory GO (E group), systemically and periodontally healthy individuals (S group). Periodontal clinical parameters were recorded. Proteomic analysis was performed on excised gingival tissues using Liquid Chromatography-Mass Spectrometry technique. The identified proteins were further classified for functional gene ontology analysis using a web-based bioinformatics. Among expressed proteins, 2 of them were confirmed by Western blotting. Statistical analysis was performed. Results: A total of 2154 proteins were detected in all samples with proteomic analysis whereas significance was revealed for 1907 proteins in multiple comparison (p<0.05). Among total proteins, 132 of them were expressed in NB, 367 in NBY, 568 in N, 366 in E, and 474 in S group. High levels of keratin type I cytoskeletal-13 (KRT-13) and Caveolin-1 in (Cav-1) were detected by Western blotting in NB and N groups respectively. Conclusion: Proteomic analysis demonstrated differential expression of proteins in the gingiva of individuals with and without NIGO. Considering the functions of verified KRT-13 and Cav-1 proteins may contribute elucidating the pathogenetic mechanism and play crucial roles in the pathogenesis of NIGO as potential biomarkers.
Clinical and Biochemical Comparison of Patients With and Without Nifedipine Induced Gingival Overgrowth PhD Student: Ece Yetiş Supervisor: Prof. Dr. Leyla Kuru Department: Periodontology Aim: In this cross-sectional study it was aimed to examine the clinical features of nifedipine induced gingival overgrowth (NIGO), investigate the pathogenetic mechanism of NIGO by proteomic analysis, in comparison with individuals using nifedipine without GO, individuals with inflammatory GO and healthy individuals. Material and Methods: The study population consisted of 54 individuals as follows, patients under nifedipine medication with (NB group) and without (N group) GO, non-overgrown regions of NIGO (NBY group), inflammatory GO (E group), systemically and periodontally healthy individuals (S group). Periodontal clinical parameters were recorded. Proteomic analysis was performed on excised gingival tissues using Liquid Chromatography-Mass Spectrometry technique. The identified proteins were further classified for functional gene ontology analysis using a web-based bioinformatics. Among expressed proteins, 2 of them were confirmed by Western blotting. Statistical analysis was performed. Results: A total of 2154 proteins were detected in all samples with proteomic analysis whereas significance was revealed for 1907 proteins in multiple comparison (p<0.05). Among total proteins, 132 of them were expressed in NB, 367 in NBY, 568 in N, 366 in E, and 474 in S group. High levels of keratin type I cytoskeletal-13 (KRT-13) and Caveolin-1 in (Cav-1) were detected by Western blotting in NB and N groups respectively. Conclusion: Proteomic analysis demonstrated differential expression of proteins in the gingiva of individuals with and without NIGO. Considering the functions of verified KRT-13 and Cav-1 proteins may contribute elucidating the pathogenetic mechanism and play crucial roles in the pathogenesis of NIGO as potential biomarkers.