Publication: Alendronatın diş çekimi sonrası ratlarda plazma biyokimyasal parametrelerine ve oksidatif strese etkisi
Abstract
Amaç: Bu çalışmada kemik rezorbsiyonunu önlemek amacıyla büyük azı dişi çekimi sonrası sistemik olarak alendronat uygulanmasının bazı biyokimyasal parametreler üzerine etkisi araştırılmıştır. İlave olarak diş çekiminin plazmada oksidatif değişiklilere etkisi olup olmadığı ve alendronat uygulanmasının bu değişiklikleri etkileyip etkilemediği incelenmiştir. Gereç ve Yöntem: ErkekWistar albino ratlar başlangıç, serum fizyolojik (kontrol) ve alendronat grubu olarak üçe ayrıldı. Ratların genel anestezi altında sağ alt çene birinci büyük azı dişleri çekildikten sonra alendronat grubuna 0.25 mg/kg alendronat kontrol grubuna serum fizyolojik subkutan olarak uygulandı. Kontrol ve deney grubundaki ratlar 14. ve 28. günlerde öldürülmeden önce kan ve idrar örnekleri alındı. Plazmalarında biyokimyasal parametreler, lipid peroksit ve indirgenmiş glutatyon (GSH) düzeyleri ile idrar kalsiyum ve kreatinin düzeyleri ölçüldü. Bulgular: Elde edilen sonuçlar değerlendirildiğinde, alendronat uygulanan grupların plazma kalsiyum düzeylerinde kontrol ve başlangıç gruplarına göre istatistiksel olarak anlamlı ölçüde düşüş görüldü (p<0.001). Alendronat diğer plazma biyokimyasal parametrelerinde anlamlı değişikliklere neden olmadı. Alendronat uygulanan gruplarda idrar kalsiyum ve idrar kreatinin düzeyleri kontrol ve başlangıç gruplarına göre anlamlı olarak azaldı (p<0.001, p<0.05). Diş çekimi sonrası oluşan mukoza hasarına bağlı plazmada gözlenen oksidatif değişiklikler istatistiksel olarak anlamlı bulunmamıştır. Bu zaman dilimlerinde alendronat uygulanmasının mukozal yaranın iyileşme sürecinde plazmadaki oksidatif stres parametreleri üzerine anlamlı bir etkisi gözlenmemiştir. Sonuç: Alendronat kullanılan dozda ve zaman dilimlerinde plazma parametrelerinde değişikliğe neden olmadan plazma ve idrar kalsiyum düzeylerini azaltır. Ayrıca mukozal yaranın iyileşme sürecinde plazmadaki oksidatif değişikliklere alendronatın etkisi yoktur.
Objective: In this study, the effect of systemic alendronate to prevent alveolar bone resorption on several biochemical parameters following molar tooth extraction was evaluated. In addition, the study was designed to verify if tooth extraction induces changes in plasma oxidative stress levels and administration of alendronate does counteract these changes. Material and Methods: MaleWistar albino rats were divided into three groups: baseline group, saline-treated group (control) and alendronatetreated group. Following the right mandibular first molar extraction in rats under general anaesthasia, the saline-treated group was administered with daily saline solution for 14 and 28 days respectively while alendronate-treated group was given a daily amount of 0.25 mg/kg alendronate subcutaneously for the same periods. Prior to sacrification on day 14 and 28, blood and urine samples were collected in control and alendronate groups. Several biochemical parameters such as lipid peroxide, reduced glutathione (GSH) levels of plasma, urinary calcium and creatinine levels were measured. Results: Our study revealed that plasma calcium levels were significantly decreased in the alendronate treated groups compared to control and baseline groups (p<0.001). However, alendronate did not cause any significant changes in other plasma biochemical parameters. Moreover, the level of urinary calcium and urinary creatinine were significantly decreased in 14 and 28 days following the extractions compared to control and baseline groups (p<0.001, p<0.05). Following tooth excraction, mucosal damage induced oxidative changes in plasma were not significant during the mucosal wound healing. Conclusion: Alendronate reduces the calcium level in both plasma and urine without causing any changes in biochemical plasma parameters. In addition, it has no effect on oxidative differences occurs in plasma during mucosal wound healing.
Objective: In this study, the effect of systemic alendronate to prevent alveolar bone resorption on several biochemical parameters following molar tooth extraction was evaluated. In addition, the study was designed to verify if tooth extraction induces changes in plasma oxidative stress levels and administration of alendronate does counteract these changes. Material and Methods: MaleWistar albino rats were divided into three groups: baseline group, saline-treated group (control) and alendronatetreated group. Following the right mandibular first molar extraction in rats under general anaesthasia, the saline-treated group was administered with daily saline solution for 14 and 28 days respectively while alendronate-treated group was given a daily amount of 0.25 mg/kg alendronate subcutaneously for the same periods. Prior to sacrification on day 14 and 28, blood and urine samples were collected in control and alendronate groups. Several biochemical parameters such as lipid peroxide, reduced glutathione (GSH) levels of plasma, urinary calcium and creatinine levels were measured. Results: Our study revealed that plasma calcium levels were significantly decreased in the alendronate treated groups compared to control and baseline groups (p<0.001). However, alendronate did not cause any significant changes in other plasma biochemical parameters. Moreover, the level of urinary calcium and urinary creatinine were significantly decreased in 14 and 28 days following the extractions compared to control and baseline groups (p<0.001, p<0.05). Following tooth excraction, mucosal damage induced oxidative changes in plasma were not significant during the mucosal wound healing. Conclusion: Alendronate reduces the calcium level in both plasma and urine without causing any changes in biochemical plasma parameters. In addition, it has no effect on oxidative differences occurs in plasma during mucosal wound healing.