Publication: Sıçanlarda ısırgan otu (Urtica dioica L.) yaprağı ile beslenmenin akut karbon tetraklorür uygulamasına bağlı gelişen karaciğer hasarı üzerine koruyucu etkisi
Abstract
Çalışmamızda, kuru ısırgan otu yaprağının, sıçanlarda karbon tetraklorür ile uyarılan lipid peroksidasyonu üzerine inhibe edici etkisi araştırıldı. Deney grupları; kontrol (grup 1), kontrol+karbon tetraklorür (grup 2) ve ısırgan otu+karbon tetraklorür (grup 3) şeklinde oluşturuldu. Grup 3 teki sıçanlar iki ay süresince kuru ısırgan otu yaprağı karıştırılmış yem ile beslendiler. Diğer gruptaki sıçanlar normal diyet aldılar. Bu süre sonunda 2. ve 3. gruptaki sıçanlara tek doz [(1ml/kg) intraperitoneal (i.p.)] CCl4 enjekte edildi. Kontrol grubuna ise yine aynı hacimde zeytinyağı intraperitoneal olarak uygulandı. Uygulamadan iki saat sonra hayvanlardan alınan kan örneklerinde plazma ALT ve AST enzim düzeyleriyle plazma lipid peroksit düzeyleri, karaciğerlerinde ise lipid peroksit düzeyleri ve glutatyon düzeyleri belirlendi. Kontrol+karbon tetraklorür sıçanlarının (grup 2) plazma ALT ve AST enzim düzeyleri, ısırgan otu+karbon tetraklorür sıçanları (grup 3) ile karşılaştırıldığında anlamlı derecede yüksek bulundu. Bu iki grubun plazma lipid peroksit düzeyleri arasında ise istatistiksel anlamda bir fark bulunmadığı gözlendi. Diğer taraftan; yine bu iki grupta ölçülen karaciğer glutatyon ve karaciğer lipid peroksit düzeyleri kıyaslandığında, ısırgan otu+karbon tetraklorür grubu sıçanlarında (grup 3) bulunan değerlerin kontrol+karbon tetraklorür (grup2) sıçanlarındakilere kıyasla anlamlı bir azalma gösterdiği belirlendi. Isırgan otu yaprağının lipid peroksidasyonunu inhibe edici etkisi bulunduğundan antioksidan etkisinin olduğu düşünülmektedir.
In this report, inhibition effect of dry Urtica dioica L. leaves on stimulated lipid peroxidation with carbon tetrachloride in rats was studied.. Animales, with a total of twenty three, were examined in 3 groups; control (group 1), control+ carbon tetrachloride (group 2) and Urtica dioica L. + carbon tetrachloride (group 3). Rats in study group 3 were fed with dry Urtica dioica L. leaves. The rats in other groups were fed with normal diet. At the end of this period, one dose of CCl4 (1ml/kg) was administered intraperitonaly to the rats in group 2. and 3. In control group, equal volume of olive oil was injected intraperitonaly to the rats. The plasma level of lipid peroxidase, ALT and AST enzymes, lipid peroxidase and glutathione levels in liver tissue were measured. The plasma level of ALT and AST of rats in control+carbon tetrachloride group (group 2) was significantly higher compared to ones in Urtica dioica L.+carbon tetrachloride group (group 3). No significant difference in peroxide level was found among these groups. On the other hand, remarkable decrease was observed in the liver glutathione and lipid peroxide levels of rats in Urtica dioica L. + carbon tetrachloride group (group 3) compared to ones in control+ carbon tetrachloride group (group 2). It was concluded that due to its inhibition effect on lipid peroxidation, Urtica Dioica L. has antioxidant properties.
In this report, inhibition effect of dry Urtica dioica L. leaves on stimulated lipid peroxidation with carbon tetrachloride in rats was studied.. Animales, with a total of twenty three, were examined in 3 groups; control (group 1), control+ carbon tetrachloride (group 2) and Urtica dioica L. + carbon tetrachloride (group 3). Rats in study group 3 were fed with dry Urtica dioica L. leaves. The rats in other groups were fed with normal diet. At the end of this period, one dose of CCl4 (1ml/kg) was administered intraperitonaly to the rats in group 2. and 3. In control group, equal volume of olive oil was injected intraperitonaly to the rats. The plasma level of lipid peroxidase, ALT and AST enzymes, lipid peroxidase and glutathione levels in liver tissue were measured. The plasma level of ALT and AST of rats in control+carbon tetrachloride group (group 2) was significantly higher compared to ones in Urtica dioica L.+carbon tetrachloride group (group 3). No significant difference in peroxide level was found among these groups. On the other hand, remarkable decrease was observed in the liver glutathione and lipid peroxide levels of rats in Urtica dioica L. + carbon tetrachloride group (group 3) compared to ones in control+ carbon tetrachloride group (group 2). It was concluded that due to its inhibition effect on lipid peroxidation, Urtica Dioica L. has antioxidant properties.