Publication: Metabolik sendromda egzersizin ve kalori kısıtlamasının kardiyovasküler hemodinamiye, erektil fonksiyona ve antioksidan sistemlere etkisi
Abstract
1.ÖZET Amaç: Çalışmanın amacı, metabolik sendromda egzersizin (EG) ve kalori kısıtlamasının (KK) kardiyovasküler hemodinamiye, erektil fonksiyona ve antioksidan sistem parametrelerine etkilerini incelemektir. Gereç ve Yöntem: Çalışmamızda 3 aylık erkek Sprague Dawley sıçanlar kullanılmıştır. Hayvanlar 5 gruba (Kontrol, MS, MS+KK, MS+EG, MS+KK+EG) ayrılmıştır. 3 ay süreyle % 10 luk furktoz solüsyonu uygulanarak metabolik sendrom modeli oluşturulmuştur. Sonrasında 6 hafta süreyle %40 kalori kısıtlaması ve yüzdürme uygulanmıştır. Deney başında, 3. ayında ve sonunda vücut ağırlıkları, kan şeker ve kan basıncı ölçümleri yapılmıştır. Dekapitasyon sonrası, kan ve doku (kalp, aort ve korpus kavernozum) örnekleri alınmıştır. Kanda sitokin değerleri, dokularda antioksidan ve oksidatif stres parametreleri incelenmiştir. Aort ve korpus kavernozum dokularında izole organ banyosunda izometrik kasılma çalışmaları yapılmıştır. Bulgular Graphpad Prism v5 erlendirilmiştir. Bulgular: Egzersiz ve kalori kısıtlaması uygulanan metabolik sendromlu hayvanlarda kan basıncında anlamlı değişiklik gösterilmiştir. Bu uygulamaların aort ve korpus kavernosum dokularının kasılma-gevşeme yanıtlarında, ayrıca malondialdehit, glutatyon ve superoksid dismutaz düzeylerinde olumlu etkileri olduğu belirlenmiştir. Sonuçlar: Egzersiz ve kalori kısıtlamasının metabolik sendromda kardiyovasküler hemodinamide ve oksidatif stres parametrelerinde iyileşme sağlamıştır. Anahtar Sözcükler: Metabolik sendrom, egzersiz, kalori kısıtlaması, erektil fonksiyon, kardiyovasküler hemodinami, antioksidan sistem 1.
Aim: The aim of this study is to examine the effects of exercise and caloric restriction to cardiovascular hemodynamics, erectile function and antioxidant system parameters in metabolic syndrome. Material and Method: Three months old male Sprague Dawley rats were used in our study. The animals were divided into five categories; (Control MS, MS+CR, MS+EXE, MS+CR+EXE). The metabolic syndrome model was generated by the use of %10 fructose solution for three months. After then, swimming exercise and %40 caloric restriction applied for six weeks. At the beginning, at the end of third month and at the end of experiment; body weight, blood sugar and blood pressure measurements were performed. At the begining and last, blood pressure of the animals were measured. After decapitation, blood and tissue (heart, aorta and corpus cavernosum) samples were collected. In blood, cytokine levels and in tissue samples antioxidant and oxidative stress parameters were examined. In isolated tissue bath, isometric contraction study was performed with aorta and corpus cavernosum tissues. Data was assessed with Graphpad Prism v5 program. Results: There was a remarkable change in the blood pressure in the animals which had calori restriction and involved in exercise program. Contraction-relaxation responses of aorta and corpus cavernosum and also melondialdehyde, glutathione, superoxide dismutase showed significant improvements. Conclusion: Exercise and caloric restriction improved cardiovascular hemodynamics and provided benefits in apoptotosis, oxidative stres parameters. Key Words: metabolic syndrome, exercise, caloric restriction, erectile function, cardiovascular hemodynamics, antioxidant system
Aim: The aim of this study is to examine the effects of exercise and caloric restriction to cardiovascular hemodynamics, erectile function and antioxidant system parameters in metabolic syndrome. Material and Method: Three months old male Sprague Dawley rats were used in our study. The animals were divided into five categories; (Control MS, MS+CR, MS+EXE, MS+CR+EXE). The metabolic syndrome model was generated by the use of %10 fructose solution for three months. After then, swimming exercise and %40 caloric restriction applied for six weeks. At the beginning, at the end of third month and at the end of experiment; body weight, blood sugar and blood pressure measurements were performed. At the begining and last, blood pressure of the animals were measured. After decapitation, blood and tissue (heart, aorta and corpus cavernosum) samples were collected. In blood, cytokine levels and in tissue samples antioxidant and oxidative stress parameters were examined. In isolated tissue bath, isometric contraction study was performed with aorta and corpus cavernosum tissues. Data was assessed with Graphpad Prism v5 program. Results: There was a remarkable change in the blood pressure in the animals which had calori restriction and involved in exercise program. Contraction-relaxation responses of aorta and corpus cavernosum and also melondialdehyde, glutathione, superoxide dismutase showed significant improvements. Conclusion: Exercise and caloric restriction improved cardiovascular hemodynamics and provided benefits in apoptotosis, oxidative stres parameters. Key Words: metabolic syndrome, exercise, caloric restriction, erectile function, cardiovascular hemodynamics, antioxidant system