Publication: Kuersetin ve siyah fermente sarımsağın doksotaksel ile kombinasyonunun karaciğer kanseri hücreleri üzerine etkisi
Abstract
Kuersetin ve Siyah Fermente Sarımsağın Doksotaksel ile Kombinasyonunun Karaciğer Kanseri Hücreleri Üzerine Etkisi Amaç: Bu çalışmada amacımız kemoterapötik ve radyoterapötiklere nispeten dirençli agresif hücre tipi olan Hep3B hücre hattında kuersetin ve siyah sarımsak ekstresinin tek başlarına ve doksotaksel ile kombinasyonunun antikanser aktivite üzerine etkisini incelemektir. Gereç ve Yöntem : Siyah sarımsaktan etanol ile siyah sarımsak ekstresi (SSE) hazırlandı. Hep3B hücreleri SSE, kuersetin, lipozomal kuersetin, ve doksotaksel ile 24 saat inkübe edildi. Sitoktoksik etkileri incelendi. Hücre canlılığı MTT testi ile belirlendi. Ayrıca SSE ve lipozomal kuersetinin doksotaksel ile değişen konsantrasyonlarda kombinasyonları ile de hücreler inkübe edildi. Kombinasyon indeksleri (CI) hesaplandı. Bulgular: Doksotaksel doz bağımlı olarak Hep3B hücrelerinde canlılığı baskıladı (p<0.001). Kuersetin Hep3B hücre canlılığı üzerine etki göstermezken lipozom formundaki kuersetin sadece 100 µg/ ml konsantrasyonda hücre canlılığını anlamlı düzeyde azalttı. Ancak lipozomal kuersetin de doksotakselle kombine teadavide sinerjik etki göstermedi. SSE, Hep3B hücre hattında hücre canlığını kontrole göre 10 (p<0.05), 25 ve 50 µg/ ml (p<0.001) konsantrasyonlarda istatistiksel olarak anlamlı düzeyde azalttı. Doksotaksel ve SSE kombine uygulandığında CI değerlerine göre doksotaksel 10 µg/ ml+SSE 5µg/ ml ve doksotaksel 25µg/ ml+SSE 5µg/ ml dozlarında istatistiksel olarak anlamlı sinerjik etki gözlendi. Sonuç: SSE, Hep3B hücre serilerinde kayda değer antikarsinojenik etkilere sahiptir ve hücrelerin doksotaksele duyarlılığını artırabilir.
The Effect of The Combination of Quercetin And Black Fermented Garlic With Docetaxel on Liver Cancer Cells Aim: Our aim in this study is to investigate the effect of the combination of quercetin and black garlic extract separately and with docetaxel on anticancer activity in Hep3B cell line, which is an aggressive cell type that is relatively resistant to chemotherapeutic and radiotherapeutics. Materials and Methods: Black garlic extract (SSE) was prepared with ethanol from black garlic. Hep3B cells were incubated with SSE, quercetin, liposomal quercetin, and docetaxel for 24 hours. Their cytotoxic effects were examined. Cell viability was determined by the MTT test. In addition, cells were incubated with varying concentrations of combinations of SSE and liposomal quercetin with docetaxel. Combination indices (CI) were calculated. Findings: Docetaxel significantly suppressed cell viability in a dose-dependent manner (p<0.001). While quercetin Hep3B had no effect on cell viability, quercetin in the form of liposomes significantly suppressed cell viability at a concentration of only 100 μg/ ml. However, liposomal quercetin did not show a synergistic effect in combination with docetaxel. SSE significantly decreased cell viability in Hep3B cell line compared to control at concentrations of 10 (p<0.05), 25 and 50 µg/ ml (p<0.001). When docetaxel and SSE were applied combined, synergistic effects were observed at docetaxel 10 μg/ ml+SSE 5μg/ ml and docetaxel 25μg/ ml+SSE 5μg/ ml doses according to CI values. Result: SSE has significant anticarcinogenic effects in Hep3B cell series and may increase the sensitivity of cells to docetaxel.
The Effect of The Combination of Quercetin And Black Fermented Garlic With Docetaxel on Liver Cancer Cells Aim: Our aim in this study is to investigate the effect of the combination of quercetin and black garlic extract separately and with docetaxel on anticancer activity in Hep3B cell line, which is an aggressive cell type that is relatively resistant to chemotherapeutic and radiotherapeutics. Materials and Methods: Black garlic extract (SSE) was prepared with ethanol from black garlic. Hep3B cells were incubated with SSE, quercetin, liposomal quercetin, and docetaxel for 24 hours. Their cytotoxic effects were examined. Cell viability was determined by the MTT test. In addition, cells were incubated with varying concentrations of combinations of SSE and liposomal quercetin with docetaxel. Combination indices (CI) were calculated. Findings: Docetaxel significantly suppressed cell viability in a dose-dependent manner (p<0.001). While quercetin Hep3B had no effect on cell viability, quercetin in the form of liposomes significantly suppressed cell viability at a concentration of only 100 μg/ ml. However, liposomal quercetin did not show a synergistic effect in combination with docetaxel. SSE significantly decreased cell viability in Hep3B cell line compared to control at concentrations of 10 (p<0.05), 25 and 50 µg/ ml (p<0.001). When docetaxel and SSE were applied combined, synergistic effects were observed at docetaxel 10 μg/ ml+SSE 5μg/ ml and docetaxel 25μg/ ml+SSE 5μg/ ml doses according to CI values. Result: SSE has significant anticarcinogenic effects in Hep3B cell series and may increase the sensitivity of cells to docetaxel.