Publication: Uzun süreli inkübasyonun ve sıcaklığın oosit aktivatörü fosfolipaz C-zeta aktivitesine etkisi
Abstract
Amaç: Kapasite olmuş spermin fertilizasyon kapasitesinin zamanla üstsel olarak düştüğü bilinmesine rağmen bunun nedeni hala araştırılmaktadır. Çalışmada amaç; uzamış inkübasyonun ve sıcaklığın sperm fertilizasyon kapasitesi üzerinde etkisini hareketlilik ve oosit aktivasyon faktörü olarak kabul edilen fosfolipaz C-zeta (PLCZ) ile değerlendirmektir.Gereç ve Yöntem: On altı infertil erkek hastadan, ciddi oligoasthenospermisi olmayan 11'inin semenlerine dansite gradyan yıkaması uygulandı. Ardından örnekler bölünerek 3 gün oda sıcaklığında veya 37°C'de kültüre edildi. Örnekler hareketlilik, PLCZ boyanma yüzdesi ve yoğunluğu açısından günlük olarak değerlendirildi.Bulgular: Hem oda sıcaklığı, hem de vücut sıcaklığında her üç parametrede de zamanla azalma gözlendi. Özellikle günler arası hareketlilikteki değişim ile PLCZ boyanan sperm yüzdesindeki değişim arasında güçlü bir korelasyon bulundu, ancak bu korelasyon zamanla azalmakta idi.Sonuç: İnkübasyon süresinin uzamasıyla, PLCZ boyanma parametreleri ve hareketlilik arasında korelasyon görülmektedir. PLCZ boyanmasının semen analizi ile rutinde kullanımı, özellikle açıklanamayan infertilite ve fertilizasyon başarısızlığı vakalarının spermlerinin fertilizasyon kapasitesinin belirlenmesinde yarar sağlayabilecek ve yardımcı üreme teknileri (YÜT) sikluslarındaki başarıyı artırabilecektir.
Objective: Fertilization capacity of capacitated sperm decreases exponentially over time, but the reason is still under investigation. The aim of the study was to analyze the effect of prolonged incubation and temperature on sperm fertilization capacity with motility and staining parameters of phospholipase C-zeta (PLCZ), which is considered to be the oocyte activation factor.Materials and Methods: Density gradient washing was applied to semen of 11 infertile patients without severe oligoasthenospermia out of 16 patients. The samples were divided and cultured either at room temperature or at 37°C for 3 days. The spermatozoons were evaluated for motility, PLCZ staining and intensity daily.Results: All parameters decreased both at room and body temperature with increased incubation time. There was a strong correlation between the change in motility and in the percentage of PLCZ stained sperms, but this correlation decreased with incubation time.Conclusion: Prolonged incubation results show the correlation between PLCZ staining parameters and motility. Routine use of PLCZ staining together with semen analysis, will be useful to predict fertilization capacity of the sperm especially for unexplained infertility and fertilization failure cases, and also can increase the success of assisted reproductive technologies (ART) cycles.
Objective: Fertilization capacity of capacitated sperm decreases exponentially over time, but the reason is still under investigation. The aim of the study was to analyze the effect of prolonged incubation and temperature on sperm fertilization capacity with motility and staining parameters of phospholipase C-zeta (PLCZ), which is considered to be the oocyte activation factor.Materials and Methods: Density gradient washing was applied to semen of 11 infertile patients without severe oligoasthenospermia out of 16 patients. The samples were divided and cultured either at room temperature or at 37°C for 3 days. The spermatozoons were evaluated for motility, PLCZ staining and intensity daily.Results: All parameters decreased both at room and body temperature with increased incubation time. There was a strong correlation between the change in motility and in the percentage of PLCZ stained sperms, but this correlation decreased with incubation time.Conclusion: Prolonged incubation results show the correlation between PLCZ staining parameters and motility. Routine use of PLCZ staining together with semen analysis, will be useful to predict fertilization capacity of the sperm especially for unexplained infertility and fertilization failure cases, and also can increase the success of assisted reproductive technologies (ART) cycles.