Publication: Spesific binding of LDL to purified GpIIb/IIIa and to isolated human platelets
| dc.contributor.authors | Tetik, S; Yardimci, KT; Uras, F; Eksioglu-Demiralp, E | |
| dc.contributor.editor | Linkesch, W | |
| dc.date.accessioned | 2022-03-12T15:58:36Z | |
| dc.date.accessioned | 2026-01-11T06:57:01Z | |
| dc.date.available | 2022-03-12T15:58:36Z | |
| dc.date.issued | 2003 | |
| dc.description.abstract | We have investigated the relationship between LDL, Fibrinogen (Fg), and GpIIb/IIIa. GpIIb/IIIa was purified from human platelets by affinity chromatography. Binding was assayed by flow cytometry. Polystyrene micro beads (Bangs Lab.) were coated with GpIIb/IIIa. Fluorescein isothiocyanate (FITC) labelled LDL, fibrinogen and BSA (negative control) were used as a ligands. Percent bound Fg-FITC or LDL-FITC to GpIIb/IIIa coated micro beads were found to be concentration dependent, reaching saturationAt saturation concentrations inhibited binding of g-FITC to GpIIb/IIIa coated micro beads dose dependently (30% max. Ynhibition). Percent bound LDL-FITC to GpIIb/IIIa coated micro beads was also inhibited by Fg (50% max. inhibition). Percent bound Fg-FITC and LDL-FITC to isolated platelets gave similar results with GpIIb/IIIa coated micro beads. Addition of purified GpIIb/IIIa caused inhibition of LDL-FITC biding to isolated platelets. GpIIb/IIIa, Fg-rcceptor on platelet is also LDL-receptor. | |
| dc.identifier.doi | doiWOS:000188281400035 | |
| dc.identifier.isbn | 88-323-3147-0 | |
| dc.identifier.uri | https://hdl.handle.net/11424/224110 | |
| dc.identifier.wos | WOS:000188281400035 | |
| dc.language.iso | eng | |
| dc.publisher | MEDIMOND S R L | |
| dc.relation.ispartof | PROCEEDINGS OF THE XVII MEETING INTERNATIONAL SOCIETY OF HAEMATOLOGY (EUROPEAN AND AFRICAN DIVISION) | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | LOW-DENSITY LIPOPROTEINS | |
| dc.subject | GLYCOPROTEIN-IIB-IIIA | |
| dc.subject | PLASMA-MEMBRANE | |
| dc.subject | RECEPTORS | |
| dc.subject | MECHANISMS | |
| dc.subject | ACTIVATION | |
| dc.subject | IIB/IIIA | |
| dc.subject | PROTEINS | |
| dc.title | Spesific binding of LDL to purified GpIIb/IIIa and to isolated human platelets | |
| dc.type | conferenceObject | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 208 | |
| oaire.citation.startPage | 205 | |
| oaire.citation.title | PROCEEDINGS OF THE XVII MEETING INTERNATIONAL SOCIETY OF HAEMATOLOGY (EUROPEAN AND AFRICAN DIVISION) |