Vascular effects of caffeic acid phenethyl ester (CAPE) on isolated thoracic aorta of ovariectomized rats.

Abstract

The present study was undertaken to investigate the effect of CAPE on isolated thoracic aorta of menopausal model rats. Fifty ovariectomized wistar/albino rats randomly divided into eight groups (n=6-7); CAPE 10 mu M endothelium (+), CAPE 10 mu M endothelium (-), CAPE 100 mu M endothelium (+), CAPE 100 mu M endothelium (-), CAPE 300 mu M endothelium (+), CAPE 300 mu M endothelium (-), ethanol and endothelium (+), ethanol and endothelium (-). After hanging into a heated organ bath, aorta rings were first stretched by 1 gram and then stretched to 4 gm in 1 gm increments in 10 minutes episodes. When the tension records were stable, contraction response using 0.1 ml NE 10(-4) M and relaxation response using 0.1 ml Ach 10(-4) M were obtained. In the experiment phase, 0.1 ml NE 10(-4) M was delivered first into each of the baths. Once contraction was reached to plateau level, 10 mu M, 100 mu M, 300 mu M CAPE were delivered into each bath respectively. In vehicle control group, responses were observed by giving thirty per cent of ethanol into the baths. There was a significant dose dependent increase in relaxation of the aorta rings with healthy and damaged endothelium. The response of CAPE 10 endothel (-) group was significantly different from the response of CAPE 300 endothel (+) group. Other groups did not display any significant difference. In the light of these results, we believe that this study can lead the way for the future work on the treatment of the cardiovascular problems of the menopause period.