Publication: Hippocampal neuronal damage in rats exposed to a double hit: ırradiation and hyperthermia
Abstract
AMAÇ: In utero radyasyon yoğun nöronal hasarı indüklemektedir. Deneysel çalışmalar, hiperterminin nöbetlere neden olduğunu ve nöronal kayba bağlı olarak yüksek mortalite oranının olduğu gösterilmiştir. Nöronal hasar ve hücre ölümüne bağlı nöron kaybı, kortikal gelişim esnasındaki hücresel gelişim değişikliğinden kaynaklanmaktadır. Bu çalışmanın amacı, radyasyon ve hipertermi uygulanmış sıçanların hipokampusundaki değişiklikleri incelemektir. YÖNTEM ve GEREÇLER: Dört deney grubu kullanılmıştır: 1) Radyasyon grubu: embriyonik 17. günde 225 cG radyasyon uygulanmıştır, 2) Hipertermi grubu: Postnatal 10. günde hipertermiye maruz bırakılmıştır, 3) Radyasyon ve hipertermi grubu: Hem radyasyon hemde hipertermi uygulanmıştır. 4) Kontrol grubu: Sıçanlar 3 ve 6 ay sonra histopatalojik olarak incelenmiştir. BULGULAR: Radyasyon grubunda hipokampusun Ca bölgelerinde nöronal kayıp, ektopik nöronlar ve hipokampal atrofi gözlenmiştir. Radyasyon ve hipertermi grubunda ki tüm hipokampal bölgelerde yoğun atrofi ile birlikte yoğun hasar görülmüştür. Uzun dönemde bütün gruplarda hasar artmıştır. SONUÇ: Bu çalışma, radyasyon ve hiperterminin hipokampus da yoğun hasar oluşturduğunu göstermektedir. İleriki çalışmalarda bu ikili modelin, hipokampal hasar oluşturmakta faydalı olacağını düşünmekteyiz.
AIm: In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. The aim of the study was to investigate the changes in hippocampus that was exposed to irradiation and hyperthermia. Ma terIal and Methods: Four groups were studied: 1) The irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) The hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) The hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) The control group was sham operated. Animals were examined 3 and 6 months later. Results: The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups. ConclusIon: This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia that may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage.
AIm: In utero irradiation models induce diffuse neuronal damage. Experimental studies have shown that hyperthermia induced seizures are easily elicited and have high mortality accompanied by neuronal loss. Neuronal damage and loss are the results of cell death coupled with cortical development in altered cellular development. The aim of the study was to investigate the changes in hippocampus that was exposed to irradiation and hyperthermia. Ma terIal and Methods: Four groups were studied: 1) The irradiation group was exposed to 225 cGy irradiation on the 17th gestational day; 2) The hyperthermia group was exposed to hyperthermia on the 10th postnatal day; 3) The hyperthermia plus irradiation group was exposed to in utero irradiation and postnatal hyperthermia; 4) The control group was sham operated. Animals were examined 3 and 6 months later. Results: The hippocampus was atrophic with neuronal loss in CA regions and ectopic neurons were in irradiation group. Severe damage with the most atrophy was demonstrated in all regions of the irradiation plus hyperthermia group. In long term, damage was severe in all groups. ConclusIon: This study demonstrated more damage in hippocampi exposed to both irradiation and hyperthermia that may be taken as an evidence for the double hit hypothesis in the development of hippocampal damage.