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A flow cytometric method of ligand binding to isolated receptors using micro beads

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dc.contributor.authorsTetik, S; Yardimci, KT; Eksioglu-Demiralp, E
dc.contributor.editorLinkesch, W
dc.date.accessioned2022-03-12T15:58:47Z
dc.date.accessioned2026-01-10T21:22:44Z
dc.date.available2022-03-12T15:58:47Z
dc.date.issued2003
dc.description.abstractThere are various methods in measuring the ligand binding to receptors. A quantitative flow cytometric method of described for binding measurements of FITC labelled ligands to receptor coated micro beads. GpIIb/IIIa complex was purified from human platelets by affinity chromatography and coated to polystyrene micro beads. Fibrinogen (Fg) was labelled with FITC. Different concentrations of Fg-FITC with GpIIb/IIIa coated micro beads were incubated and analyzed by flow cytometry. BSA-FITC was used as a negative control. The Fg-FITC binding to isolated platelets was also studied under the same conditions. This method besides showing quantitative ligand binding to the receptor, it also reflects the in vivo binding conditions.
dc.identifier.doidoiWOS:000188281400037
dc.identifier.isbn88-323-3147-0
dc.identifier.urihttps://hdl.handle.net/11424/224156
dc.identifier.wosWOS:000188281400037
dc.language.isoeng
dc.publisherMEDIMOND S R L
dc.relation.ispartofPROCEEDINGS OF THE XVII MEETING INTERNATIONAL SOCIETY OF HAEMATOLOGY (EUROPEAN AND AFRICAN DIVISION)
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFIBRINOGEN BINDING
dc.subjectACTIVATED PLATELETS
dc.subjectWHOLE-BLOOD
dc.subjectAGGREGATION
dc.subjectEXPRESSION
dc.subjectIIIA
dc.titleA flow cytometric method of ligand binding to isolated receptors using micro beads
dc.typeconferenceObject
dspace.entity.typePublication
oaire.citation.endPage218
oaire.citation.startPage215
oaire.citation.titlePROCEEDINGS OF THE XVII MEETING INTERNATIONAL SOCIETY OF HAEMATOLOGY (EUROPEAN AND AFRICAN DIVISION)

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