Cell Growth on In Situ Photo-Cross-Linked Electrospun Acrylated Cellulose Acetate Butyrate

Abstract

In this study, electrospinning was combined with UV curing technology for producing in situ photo crosslinked fibers from methacrylated cellulose acetate butyrate (CABIEM). ECV304 and 3T3 cells were seeded on electrospun fibrous scaffolds. Collagen modified CABIEM fibers were also prepared for improving cell adhesion and proliferation. Cross-linking and the morphology of the fibers were characterized by ATR-FT-IR spectrometry and environmental scanning electron microscopy (ESEM). The cytotoxicity of the fibers was examined using the MTT cytotoxicity assay. According to the results, electrospun fibrous scaffolds are non-toxic and cell viability depends on the amount of collagen. It was found that cell adhesion and cell growth were enhanced as the collagen percentage was increased. (C) Koninklijke Brill NV, Leiden, 2012