Three-Dimensional Polycaprolactone Scaffold Combined with Tetracycline-Loaded Electrospray Particles

Abstract

The methods used in drug transport systems have been replaced by many different and advanced modeling systems with the developing technology. The treatment methods developed as a result of combining synthetic and natural polymers with new generation nanotechnological products such as 3D devices lead studies in interdisciplinary fields such as medical science, pharmacy and bioengineering.[1] Drug delivery systems are one of the popular fields of study where the maximum therapeutic efficacy of active ingredients is investigated. Nanotechnological developments aim to realize drug release more effectively. [2] Electrospray method is a more preferred option than conventional methods for this target. Electrospray particles composed of different polymers or polymer blends have been investigated as potential carriers for drug delivery and release. [3] In this study, a total of 7 layers of polymer scaffolds containing PCL were prepared using a 3D printer. Tetracillin-loaded PVP particles were electrosprayed into layers 2, 5 and 7 of the 7-layer PCL polymer scaffold. These spherical particles were optimized in a 1:1 solution of % 13 w/v PVP dissolved in chloroform and DCM. The resulting scaffold and particles had a layered structure. The particles sprayed on different layers of the scaffold were optimized at 8 cm needle tip and collector distance, 0.8 ml/h flow rate and 13.5 kV voltage. Scanning electron microscopy (SEM) was applied for morphological analysis of particle-loaded scaffolds, Fourier transform infrared spectroscopy (FT-IR) to observe chemical bonds and molecular interaction, and differential scanning calorimetry (DSC) tests to examine thermal behavior. In addition, the drug release test of tetracillin was performed with a UV spectrophotometer. Inspired by the sandwich modeling design, this model, which has 2 different designs, is a potential candidate method that can be used in a more effective drug release profile for antibiotic group drugs.