Publication: Akciğerin iskemi-reperfüzyon hasarını engellemede teofilinin yeri ve uygun dozu
Abstract
Bu çalışmada bir metilksantin türevi olan teofilinin akciğerde iskemi-reperfüzyon hasarı üzerine olan etkileri ve hasarı azaltmadaki uygun perfüzyon dozu araştırılmıştır. 30 sıçan üzerinde uyguladığımız bu deneysel çalışmada, deney grubu 1'de (n:5) 20 mg/L, deney grubu 2'de (n:5) 100 mg/L, deney grubu 3'te (n:5) 400 mg/L ve deney grubu 4'te (n:5) 1000 mg/L teofilin içeren koruma solüsyonu perfüzyonu sonrası 6 saat süreyle hipotermik iskemide tutulan ve 30 dakika boyunca %100 O2 ile ventile edilerek reoksijenasyon sağlanan izole sıçan akciğerlerinde lipid peroksidasyonu ara ürünü olan dien konjugat (DC) ve malondialdehit (MDA) doku düzeyleri ölçüldü ve 2 adet kontrol grubu ile karşılaştırıldı. 400-1000 mg/L teofilin içeren solüsyonlarla yapılan korumada DC ve MDA düzeyleri istatistiksel anlamlı olarak düşük bulundu. Bu çalışmada elde edilen veriler değerlendirildiğinde; koruma solüsyonuna eklenen 400 - 1000 mg/L oranında teofilinin donör akciğerinde hipotermik iskemiye bağlı olarak gelişen oksidatif hasarı anlamlı derecede azalttığı sonucuna varılmıştır.
In this study, the role and the right dosage of theophylline, a methylxanthine derivative, in the prevention of ischemia-reperfusion injury of the lung has been investigated. This experimental study was permormed with 30 rats; in experimental group (EG) 1 (n:5) 20 mg/L, in EG 2 (n:5) 100 mg/L, in EG 3 (n:5) 400 mg/L and in EG 4 (n:5) 1000 mg/L theophylline added flush solution were perfused. Lungs were kept in hypothermic state for 6 hours and ventilated for 30 minutes with 100 % O2. Tissue levels of dien congugate (DC) and malondialdehyde (MDA) - intermediate products of lipid peroxidation- were measured. The results were compared with 2 control groups. Dien congugate and MDA levels in the groups preserved with 400-1000 mg/L were significantly less than the other groups. The results of this study showed that 400-1000 mg/L of theophylline in the flush solution decreased the ischemic injury.
In this study, the role and the right dosage of theophylline, a methylxanthine derivative, in the prevention of ischemia-reperfusion injury of the lung has been investigated. This experimental study was permormed with 30 rats; in experimental group (EG) 1 (n:5) 20 mg/L, in EG 2 (n:5) 100 mg/L, in EG 3 (n:5) 400 mg/L and in EG 4 (n:5) 1000 mg/L theophylline added flush solution were perfused. Lungs were kept in hypothermic state for 6 hours and ventilated for 30 minutes with 100 % O2. Tissue levels of dien congugate (DC) and malondialdehyde (MDA) - intermediate products of lipid peroxidation- were measured. The results were compared with 2 control groups. Dien congugate and MDA levels in the groups preserved with 400-1000 mg/L were significantly less than the other groups. The results of this study showed that 400-1000 mg/L of theophylline in the flush solution decreased the ischemic injury.