Publication: İnvestigation of carbamazepine-fe(III) and 10,11 dihydroxcycarbamazepine- fe(III) interactions with spectrophotometric method
Abstract
Karbamazepin (CBZ) yapısı bakımından imipramin 'e benzeyen antiepileptik olarak kullanılan bir ilaçtır. Bu çalışmada CBZ-Fe(III) ve karbamazapinin önemli metabolitlerinden biri olan 10,11 dihidrosikarbamazapin (CBZ-diol) - Fe(III) arasındaki kimyasal etkileşim in-vitro ortamda incelenerek, CBZ ve metabolitinin demir ile kompleks oluşturan maddelere benzer biçimde demir eksikliğine bağlı anemiye neden olup olamayacağı yönünde kimyasal veriler elde edilmeye çalışıldı. CBZ-Fe (III) arasındaki kimyasal etkileşinîin en iyi % 10 dioksan-su çözücü ortamında, pH 2-3 aralığında, oda sıcaklığında, 5 dakika reaksiyon süresinde ve eşit konsantrasyonda CBZ ve Fe (III) ile gerçekleştiği, 10,11 CBZ-diol - Fe(III) arasındaki kimyasal etkileşimin ise en iyi sulu çözeltide, oda sıcaklığında, 5 dakika reaksiyon süresinde, pH 2-3 aralığında ve demirin iki katı CBZ konsantrasyonunda gerçekleştiği bulundu. Ayrıca, etkileşim reaksiyonunun bir kompleksleşme reksiyonu olup olmadığı Irwing-Rossoti metoduna göre potansiyometrik yöntemle incelendi ve CBZ—Fe (III) ve CBZ-diol—Fe(III) arasındaki etkileşimlerin kompleksleşme reaksiyonu değil zayıf bir etkileşim reaksiyonu olabileceği yönünde spektrofotometrik sonuçları destekleyen sonuçlar elde edildi.
Carbamazepine (CBZ) is an antiepileptic drug, with a structure similar to imipramine. In this study, chemical interaction of carbamazepine and 10,11 dihydroxycarbamazepine (CBZ-diol) which is one of the most important metabolites of carbamazepine with Fe(III) were investigated in in-vitro media in order to examine anemia owing to iron deficiency. The data are obtained via investigation of the compounds that form complexes with iron. As a result of spectrophotometric investigation of the chemical interaction between CBZ and Fe (III) was found to occur in % 10 dioxane - water solvent medium, at the interval ofpH= 2-3, at room temperature, in 5 min reaction time, when equal concentrations of CBZ and Fe (III) were used. The interaction reaction of 10,11 CBZ-diol with Fe (III) was found to occur optimally in aqueous solution at room temperature, in 5 min reaction time, at the interval of pH=2-3 and concentration of CBZ two times higher than Fe (III) concentration. It is investigated that whether the interaction reaction of Fe(III)- CBZ and Fe(III)-CBZ-diol to make complexes according to Irwing-Rossoti method by potentiometry. The interaction between CBZ- Fe(III) and CBZ-diol- Fe(III) is a weak interaction reaction rather than being a reaction that forms complexes and this supports the results of spectrophotometric analysis.
Carbamazepine (CBZ) is an antiepileptic drug, with a structure similar to imipramine. In this study, chemical interaction of carbamazepine and 10,11 dihydroxycarbamazepine (CBZ-diol) which is one of the most important metabolites of carbamazepine with Fe(III) were investigated in in-vitro media in order to examine anemia owing to iron deficiency. The data are obtained via investigation of the compounds that form complexes with iron. As a result of spectrophotometric investigation of the chemical interaction between CBZ and Fe (III) was found to occur in % 10 dioxane - water solvent medium, at the interval ofpH= 2-3, at room temperature, in 5 min reaction time, when equal concentrations of CBZ and Fe (III) were used. The interaction reaction of 10,11 CBZ-diol with Fe (III) was found to occur optimally in aqueous solution at room temperature, in 5 min reaction time, at the interval of pH=2-3 and concentration of CBZ two times higher than Fe (III) concentration. It is investigated that whether the interaction reaction of Fe(III)- CBZ and Fe(III)-CBZ-diol to make complexes according to Irwing-Rossoti method by potentiometry. The interaction between CBZ- Fe(III) and CBZ-diol- Fe(III) is a weak interaction reaction rather than being a reaction that forms complexes and this supports the results of spectrophotometric analysis.