Cytotoxicity and Collagen Expression Effects of Tideglusib Administration on Human Periodontal Cells: An In-Vitro Study

Abstract

Objective: Tideglusib is a GSK-3 inhibitor activating Wnt/beta-catenin signaling pathway which has significant importance in regenerative response. The aim of this study was to evaluate the cytotoxicity and protein expression impacts of Tideglusib on human periodontal cell lines. Methods: Cytotoxicity effect of different concentrations (50nM, 100nM, 200nM) of Tideglusib application on human gingival fibroblast (hGF), periodontal ligament fibroblast (hPDLF), and osteoblast (hOB) cell lines was determined. Type-I and III collagen expressions were evaluated after 24-hour application of 50nM Tideglusib. Results: The cytotoxicity of 200nM Tideglusib was higher in hGF and hOB (p<0.05), but no difference was found in hPDLF compared to the respective control group (p>0.05). The hGF and hOB treated with 50nM Tideglusib expressed an increased level of Type-I collagen (p<0.05), but no difference was detected in the hPDLF compared to the respective control (p>0.05). Type-III collagen expressions were similar between the test and control groups for each cell line (p>0.05). Conclusion: Tideglusib is not cytotoxic at 50nM and 100nM concentrations and may have positive effect on bone regeneration rather than periodontal regeneration since it stimulated Type-I collagen production in hGF and hOB cells, but not in hPDLF.