Publication: B tipi hepatit zemininde gelişen hepatosellüler kanserde E-cadherin ekspresyonu: Normal, kronik hepatit ve sirozla karşılaştırılması
Abstract
Cadherin’ler hücre-hücre ilişkisinde önemli, kalsiyum bağımlı transmembran proteinlerdir. E-cadherin’ler ise epitelyal hücrelerde eksprese edilen bir subgruptur. E-cadherin ekspresyonu kaybolan hücrelerin davranışı anormalleşir. Biz bu çalışmada hepatitis B zemininde gelişen hepatosellüler kanserde e-cadherin ekspresyonunu araştırdık. Elde ettiğimiz sonuçları normal, kronik hepatit B ve hepatit B sirozu ile karşılaştırdık. Çalışma kapsamına, 5 normal, 15 kronik B hepatiti, 8 hepatit B sirozu ve 8 hepatit B zemininde gelişmiş hepatosellüler kansere ait doku örnekleri alındı. Doku kesitlerine e-cadherin, streptavidin-biotin-alkalen fosfataz immünohistokimya yöntemiyle boyandı. E-cadherin ekspresyonu, % 10’dan az (+), % 10-50 (++), % 50-90 (+++), % 90’in üstü (++++) olmak üzere, hepatositlerin boyanma oranlarina göre semikantitatif olarak değerlendirildi. Normal karaciğer dokusunda hepatositlerin ve bilier epitel hücrelerinin % 90’nında E-cadherin ekspresyonu görüldü. Kanserli olgularda e-cadherin ekspresyonu normal, kronik hepatit ve siroza göre anlamlı oranda azalmış bulundu (p<0.05). Çalışmamız E-cadherin ekspresyonundaki azalmanın hepatosellüler karsinom patogenezinde proliferasyon artışına neden olarak etkili olabileceğini düşündürmektedir.
Cadherins are calcium dependent transmembrane proteins, which play an important role in cell to cell interaction. E-cadherins are subgroups of cadherins which are expressed in epithelial cells. A decrease in E-cadherin expression may cause abnormal behavior. We investigated E-cadherin expression in hepatitis B virus related hepatocellular carcinoma and compared this expression with normal, chronic hepatitis B and hepatitis B related cirrhosis. Five normal, 15 chronic hepatitis B, eight hepatitis B cirrhosis and eight hepatitis B related hepatocellular carcinoma tissue samples were included in this study. All tissue samples were stained for E-cadherin by streptavidin-avidin-alkaline phosphatase immunochemical method. E-cadherin expression was scored semiquantitatively (by percent age of hepatocytes stained) as (+) if less than 10% , (++) if 10-50% , (+++) if 50-90% and (++++) if more than 90%. In normal liver, 90% of hepatocytes and biliary epithelial cells showed E-cadherin expression. E-cadherin expression was significantly lower in cancer patients compared to normal, chronic hepatitis and cirrhosis patients (p<0.05). In conclusion, decrease in E-Cadhenin expression may have an important role in the pathogenesis of hepatocellular carcinoma by leading to an increase in the proliferation rate of hepatocyes.
Cadherins are calcium dependent transmembrane proteins, which play an important role in cell to cell interaction. E-cadherins are subgroups of cadherins which are expressed in epithelial cells. A decrease in E-cadherin expression may cause abnormal behavior. We investigated E-cadherin expression in hepatitis B virus related hepatocellular carcinoma and compared this expression with normal, chronic hepatitis B and hepatitis B related cirrhosis. Five normal, 15 chronic hepatitis B, eight hepatitis B cirrhosis and eight hepatitis B related hepatocellular carcinoma tissue samples were included in this study. All tissue samples were stained for E-cadherin by streptavidin-avidin-alkaline phosphatase immunochemical method. E-cadherin expression was scored semiquantitatively (by percent age of hepatocytes stained) as (+) if less than 10% , (++) if 10-50% , (+++) if 50-90% and (++++) if more than 90%. In normal liver, 90% of hepatocytes and biliary epithelial cells showed E-cadherin expression. E-cadherin expression was significantly lower in cancer patients compared to normal, chronic hepatitis and cirrhosis patients (p<0.05). In conclusion, decrease in E-Cadhenin expression may have an important role in the pathogenesis of hepatocellular carcinoma by leading to an increase in the proliferation rate of hepatocyes.