Publication:
Ghrelin improves burn-induced multiple organ injury by depressing neutrophil infiltration and the release of pro-inflammatory cytokines

dc.contributor.authorYEGEN, BERRAK
dc.contributor.authorERZİK, CAN
dc.contributor.authorÇETİNEL, ŞULE
dc.contributor.authorŞENER, TARIK EMRE
dc.contributor.authorsSehirli, Oezer; Sener, Emre; Sener, Goeksel; Cetinel, Sule; Erzik, Can; Yegen, Berrak C.
dc.date.accessioned2022-03-12T17:33:55Z
dc.date.accessioned2026-01-10T16:53:50Z
dc.date.available2022-03-12T17:33:55Z
dc.date.issued2008
dc.description.abstractMechanisms of burn-induced skin and remote organ injury involve oxidant generation and the release of pro-inflammatory cytokines. In this study the possible antioxidant and anti-inflammatory effects of ghrelin were evaluated in a rat model of thermal trauma. Wistar albino rats were exposed to 90 degrees C bath for 10 s to induce thermal trauma. Ghrelin, was administered subcutaneously (10 ng/kg/day) after the burn injury and repeated twice daily. Rats were decapitated at 6 h and 48 h after burn injury and blood was collected for the analysis of pro-inflammatory cytokines (TNF-alpha and IL-1 beta), lactate dehydrogenase (LDH) activity and antioxidant capacity (AOC). In skin, lung and stomach tissue samples malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) and Na+-K+-ATPase activity were measured in addition to the histological analysis. DNA fragmentation ratio in the gastric mucosa was also evaluated. Burn injury caused significant increase in both cytokine levels, and LDH activity, while plasma ACC was found to be depleted after thermal trauma. On the other hand, in tissue samples the raised MDA levels, MPO activity and reduced GSH levels, Na+-K+-ATPase activity due to burn injury were found at control levels in ghrelin-treated groups, while DNA fragmentation in the gastric tissue was also reduced. According to the findings of the present study, ghrelin possesses a neutrophil-dependent anti-inflammatory effect that prevents burn-induced damage in skin and remote organs and protects against oxidative organ damage. (C) 2008 Elsevier Inc. All rights reserved.
dc.identifier.doi10.1016/j.peptides.2008.02.012
dc.identifier.eissn1873-5169
dc.identifier.issn0196-9781
dc.identifier.pubmed18395937
dc.identifier.urihttps://hdl.handle.net/11424/228935
dc.identifier.wosWOS:000257489100019
dc.language.isoeng
dc.publisherELSEVIER SCIENCE INC
dc.relation.ispartofPEPTIDES
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectthermal trauma
dc.subjectghrelin
dc.subjectcytokine
dc.subjectlipid peroxidation
dc.subjectmyeloperoxidase
dc.subjectLIPID-PEROXIDATION
dc.subjectACYLATED PEPTIDE
dc.subjectTHERMAL-INJURY
dc.subjectRATS
dc.subjectACTIVATION
dc.subjectAPOPTOSIS
dc.subjectGLUTATHIONE
dc.subjectMECHANISMS
dc.subjectEXPRESSION
dc.subjectETHANOL
dc.titleGhrelin improves burn-induced multiple organ injury by depressing neutrophil infiltration and the release of pro-inflammatory cytokines
dc.typearticle
dspace.entity.typePublication
oaire.citation.endPage1240
oaire.citation.issue7
oaire.citation.startPage1231
oaire.citation.titlePEPTIDES
oaire.citation.volume29

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