Publication: Tamoksifenin sıçan karaciğerine etkisi: Işık ve elektron mikroskopik inceleme
Abstract
Tamoksifen, meme kanserinin tedavisinde uzun süreli, en çok kullanılan trifeniletilen grubundan, en etkili antiöstrojenik ilaçtır. Bu ilacın uzun süreli kullanımına bağlı olarak karaciğer ve uterusta yan etkilere neden olduğu bilinmektedir. Karaciğer üzerine morfolojik etkilerini incelemek amacı ile Sprague-Dawley cinsi dişi sıçanlara 3.5 ay süreyle i.p olarak tamoksifen, 1.5 mg /kg olacak şekilde uygulandı. Alınan karaciğer biyopsi materyalleri ışık ve elektron mikroskopi düzeyinde incelendi. Işık mikroskopi düzeyinde karaciğer hücre kordon düzeninde bozulma, kanama odakları, Disse aralığı bağ dokusunda artış gözlendi. Hepatosit ince yapısında membran ve organellerinde değişiklikler, glikojen içeriğinde ve lipid damlacıklarında ve vakuollerde artma gözlendi. Sonuç olarak, sıçanlarda bu süre ve dozda tamoksifen uygulanımı karaciğer hasarına sebep olmaktadır.
The effects of tamoxifen on the rat liver; light and electron microscopical study. Tamoxifen is an antioestrogenic in a triphenylethylen group drug, common used for long time period mammary gland cancer treatment. Long time period consumption of this drug has a negative effect on the uterus and liver. For observation of the effect of tamoxifen on the liver morphology, tamoxifen was applied to the Sprague-Dawley female rats ip. 1.5 mg/kg for 3.5 months. Liver samples were examined at both light and electron microscopy level. Liver samples was showed that degeneration of the regulation of the cell cordon, bleeding areas, increase of the connective tissue at Disse space at light microscopy level. Ultrastructural observations showed that alterations of the membrane and organelle structure, formation of vacuoles, and increase of glycogen and lipid droplets in the hepatocyte cytoplasm. As a result, application of the tamoxifen to the rats in these doses and time causes liver degeneration. As a result, patient treated with tamoxifen because of the observation of the liver degeneration should be under control frequently.
The effects of tamoxifen on the rat liver; light and electron microscopical study. Tamoxifen is an antioestrogenic in a triphenylethylen group drug, common used for long time period mammary gland cancer treatment. Long time period consumption of this drug has a negative effect on the uterus and liver. For observation of the effect of tamoxifen on the liver morphology, tamoxifen was applied to the Sprague-Dawley female rats ip. 1.5 mg/kg for 3.5 months. Liver samples were examined at both light and electron microscopy level. Liver samples was showed that degeneration of the regulation of the cell cordon, bleeding areas, increase of the connective tissue at Disse space at light microscopy level. Ultrastructural observations showed that alterations of the membrane and organelle structure, formation of vacuoles, and increase of glycogen and lipid droplets in the hepatocyte cytoplasm. As a result, application of the tamoxifen to the rats in these doses and time causes liver degeneration. As a result, patient treated with tamoxifen because of the observation of the liver degeneration should be under control frequently.