FORMATION AND HPLC ANALYSIS OF THE NATURAL LAKE PIGMENT OBTAINED FROM MADDER (RUBIA TINCTORUM L.)

Abstract

In this work, madder lake pigments, aluminium-madder, tin-madder and iron-madder, were obtained by the reaction of KAI(SO(4))(2)center dot 12H(2)O, FeSO(4)center dot 7H(2)O and SnC12 center dot 2H(2)O solutions with madder. High performance liquid chromatography (HPLC) with diode-array detection (DAD) method was utilized for the identification of the lake pigments. Reversed-phase HPLC with diode-array UV-V is spectrophotometric detection was used in this identification. The extraction of dyestuffs from the lake pigments was carried out with HCI / methanol / water (2:1:1; v/v/v) solution. From the results of the HPLC analysis of the madder lake pigments, it was determined that the alizarin present in the lake pigments was precipitated by A1(III), Fe(II) and Sn(II).