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Interaction of 3H-defibrotide with cultured human umbilical vein endothelial cells

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dc.contributor.authorYALÇIN, AHMET SUHA
dc.contributor.authorsBilsel, S.; Yalçin, A. S.; Taga, Y.; Emerk, K.
dc.date.accessioned2022-03-15T11:11:00Z
dc.date.accessioned2026-01-10T19:29:34Z
dc.date.available2022-03-15T11:11:00Z
dc.date.issued1990
dc.description.abstractDefibrotide is a profibrinolytic and antithrombotic drug which seems to modulate endothelial cell function. In this study, a method for radioactive labeling of the drug and its interaction with cultured endothelial cells is proposed. 3H-Acetic anhydride was used to label defibrotide. Endothelial cells obtained by collagenase treatment of human umbilical cord veins were cultured in 24-welled plastic culture dishes. Binding experiments were carried out by incubating cell cultures with media containing various concentrations of labeled defibrotide. Our results showed that labeled defibrotide has a KL value of 4.2 micrograms/ml for endothelial cells. Although the presence of a specific transporter is possible, the high molecular weight of the fraction used suggests that the interaction is binding to a specific receptor.
dc.identifier.doi10.1016/0049-3848(91)90250-z
dc.identifier.issn0049-3848
dc.identifier.pubmedPMID: 2368032
dc.identifier.urihttps://hdl.handle.net/11424/248827
dc.language.isoeng
dc.relation.ispartofThrombosis Research
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHumans
dc.subjectKinetics
dc.subjectCells, Cultured
dc.subjectEndothelium, Vascular
dc.subjectFibrinolytic Agents
dc.subjectPolydeoxyribonucleotides
dc.subjectBinding Sites
dc.titleInteraction of 3H-defibrotide with cultured human umbilical vein endothelial cells
dc.typearticle
dspace.entity.typePublication
oaire.citation.endPage460
oaire.citation.startPage455
oaire.citation.titleThrombosis Research
oaire.citation.volume5

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