Publication: The Persistence and Clearance Rate of Human Papillom Virus Genotypes in Urban Turkish Women after One Yea
Abstract
Amaç: Marmara Üniversitesi Hastanesi kadın hastalıkları polikliniğinde human papilloma virüs (HPV) pozitif saptanan kadınlarda değişik HPV genotiplerinin persistansını değerlendirmek. Hastalar ve Yöntem: İlk prevalans çalışmasında HPV DNA testi pozitif saptanan 79 kadından 40ı bir yıl sonra tekrar değerlendirildi. HPV tipleri polymerase chain reaction (PCR) ve mikroarray hibridizasyon teknikleri ile tanımlandı. Bulgular: İlk değerlendirmenin bir yıl sonrasında kadınların %52,5nin HPV enfeksiyonunun ortadan kalktığı; %35 inin yeni bir HPV enfeksiyonu edindiği saptandı. 40 kadının %17,5inde HPV DNA persistansı saptandı. Dokuz kadın aynı filogenetik ağaçtan HPV genotipi edinmişti. Yüksek riskli (YR) HPV tipi enfeksiyonun %43,33ü ve düşük riskli (DR) HPV tipi enfeksiyonun %80i ortadan kalkmıştı. Sonuç: Yüksek riskli HPV tipleri saptanan kadınlarda persistans oranı artmıştı. Çok sayıda ve karışık tipte HPV enfeksiyonları HPV genotipinin persistansında önemli etkiye sahiptir. (Marmara Üniversitesi Tıp Fakültesi Dergisi 2012;25:10-5)
Objective: To evaluate the persistence of the different human papillomavirus (HPV) genotypes in women detected positive for HPV at Marmara University Hospital gynecologic outpatient clinics. Patients and Methods: Forty out of 79 women who had been tested positive for HPV DNA in our initial prevalance study were re-assessed after one year. HPV types were identified by polymerase chain reaction (PCR) and hybridization using a microarray. Results: One year after the initial assessment, 52.5% of the women had their initial HPV infection resolved and 35% of the women had acquired another HPV infection. The HPV DNA persistence was detected in 17.5% of the 40 women. Nine women had acquisition of HPV genotype by the same phylogenetic clade. 43.33% of high risk (HR) HPV type and 80% of the low risk (LR) HPV type infection had resolved. Conclusions: The persistence rate was increased in women with HR HPV types. Multiple and mixed HPV infections have an important impact on the persistence of HPV genotype. (Marmara Medical Journal 2012;25:10-5) Key Words: Cervical cytology, HPV genotype, HPV persistence, HPV clearance
Objective: To evaluate the persistence of the different human papillomavirus (HPV) genotypes in women detected positive for HPV at Marmara University Hospital gynecologic outpatient clinics. Patients and Methods: Forty out of 79 women who had been tested positive for HPV DNA in our initial prevalance study were re-assessed after one year. HPV types were identified by polymerase chain reaction (PCR) and hybridization using a microarray. Results: One year after the initial assessment, 52.5% of the women had their initial HPV infection resolved and 35% of the women had acquired another HPV infection. The HPV DNA persistence was detected in 17.5% of the 40 women. Nine women had acquisition of HPV genotype by the same phylogenetic clade. 43.33% of high risk (HR) HPV type and 80% of the low risk (LR) HPV type infection had resolved. Conclusions: The persistence rate was increased in women with HR HPV types. Multiple and mixed HPV infections have an important impact on the persistence of HPV genotype. (Marmara Medical Journal 2012;25:10-5) Key Words: Cervical cytology, HPV genotype, HPV persistence, HPV clearance