Publication: Uzun vas deferens (VD) kayıplarının onarımında VD ve ven ototransplantasyon seçeneklerinin sıçan modelinde karşılaştırılması
Abstract
"Vas deferens (VD)'in uzun segment kayıplarının sağaltımı halen tartışmalıdır. Çalışmamızın amacı; bu tip VD kayıplarının yerine konmasında iki ayrı greft tekniğinin (vas deferens ve ven) bir sıçan modelinde öncelikle uygulanabilirliklerinin araştırılması ve birbirleri ile karşılaştırılmalarıdır. Bu amaçla 24 Wistar-Albino cinsi sıçan iki eşit gruba ayrıldı. Grup A'da oluşturulan uzun VD kaybının onarımı sağ tarafta karşı taraftan çıkarılan segmentin ototransplantasyonu (VDtr) ile solda ise vazovazostomi (VV) ile gerçekleştirildi. Grup B'de, sağ tarafta A gru-bununkine benzer şekilde VD kaybı oluşturulduktan sonra 1 cm uzunluğunda ven segmenti araya interpoze edildi (Vtr), solda sham ameliyatı yapıldı. Sıçanlar dört haftalık iyileşme döneminin sonunda sakrifiye edildiler ve kanal açıktıklığı, debi hızı histolojik değişiklikler yönünden değerlendirme yapıldı. Spermatogenezis "Johnsen'ın Testis Biopsy Score Count" ile değerlendirildi. VD transplantlarının tümü canlılıklarını ve duvar yapılarını korumuşken ven greftlerinin duvarında ağır dejenerasyon bulguları vardı. VD transplantlarının kanal açıklık oranı % 66.6 idi, Vtr grubunda ise açık greft kanalı hiç yoktu. Debi değerleri, VDtr (0.4-2.4 ccldk) ve sham (1.3-2.5 cc/dk) tarafları arasında farklı bulunmakla birlikte istatistiksel olarak anlamlı değildi (p>0.05). VD transplantlarının, devaskülarizasyondan 4 hafta sonra canlılıklarını yeterli kanal açıklığı ile korumuş olmalarından ötürü, "VD transplantasyonu" nun uzun VD kayıplarında yeni tedavi se¬çeneklerinin araştırılmasında kullanılabilecek bir yöntem olduğunu düşünüyoruz.
Comparison of two different autotransplantation techniques in repair of vas deferens large defects in rat: Vas deferens versus vein Management of vas deferens (VD) large defects are still controversial. The aim of our study was to compare two different substitution techniques in repair of VD large de¬fects: VD transplantation (VDtr) versus vein interposition (Vtr). Twenty four Wistar-Albino rats were divided into two groups. In group A repair of the established VD de¬fects were performed by VDtr of the left segment on the right and vasovasostomy (VV) on the left side. In group B a VD defect was established on the right side as in group A, and a segment of 1 cm jugular vein was interposed to achive lumen contunity of right VD, while a sham proce¬dure was done on the left. All the rats were sacrified after 4 weeks of recovery period. The specimens were evaluated lumen patency, flow rates and histologic alterations of the VD segments. Testis Biopsy Score Counts were calculated for accurate evaluation of spermatogenesis. All the VD transplants kept their viability, while vein transplants re¬vealed severe degeneration of the wall. Patency of trans¬plants were 66.6 % for VDtr group and there was no pa¬tent Vtr segment. Flow rates were different between the VDtr (0.4-2.4 cc/min) and sham segments (1.3-2.5 cclmin) but were not statistically significant (p>0.05). As all transplanted VD segments were viable with adequate lumen patency after four weeks of devascularization, we concluded that this technique could be used to explore new ways to repair of VD large defects.
Comparison of two different autotransplantation techniques in repair of vas deferens large defects in rat: Vas deferens versus vein Management of vas deferens (VD) large defects are still controversial. The aim of our study was to compare two different substitution techniques in repair of VD large de¬fects: VD transplantation (VDtr) versus vein interposition (Vtr). Twenty four Wistar-Albino rats were divided into two groups. In group A repair of the established VD de¬fects were performed by VDtr of the left segment on the right and vasovasostomy (VV) on the left side. In group B a VD defect was established on the right side as in group A, and a segment of 1 cm jugular vein was interposed to achive lumen contunity of right VD, while a sham proce¬dure was done on the left. All the rats were sacrified after 4 weeks of recovery period. The specimens were evaluated lumen patency, flow rates and histologic alterations of the VD segments. Testis Biopsy Score Counts were calculated for accurate evaluation of spermatogenesis. All the VD transplants kept their viability, while vein transplants re¬vealed severe degeneration of the wall. Patency of trans¬plants were 66.6 % for VDtr group and there was no pa¬tent Vtr segment. Flow rates were different between the VDtr (0.4-2.4 cc/min) and sham segments (1.3-2.5 cclmin) but were not statistically significant (p>0.05). As all transplanted VD segments were viable with adequate lumen patency after four weeks of devascularization, we concluded that this technique could be used to explore new ways to repair of VD large defects.