Publication: Cerrahisiz periodontal tedavinin makrofaj ilişkili moleküller üzerine etkisi
Abstract
Amaç: Farklı periodontal duruma sahip bireylerde; tükürük ile serum matriks metalloproteinaz-8 (MMP-8), makrofaj koloni stimüle edici faktör (M-CSF), makrofaj enflamatuvar protein-1α (MIP-1α), makrofaj aktive edici faktör (MAF), interlökin-34 (IL-34) seviyelerinin karşılaştırılması ve cerrahisiz periodontal tedavinin (CPT) bu moleküller üzerine etkisinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamıza 20 sağlıklı, 20 gingivitis, 20 periodontitis evre III derece B (P-III-B) ve 25 periodontitis evre III derece C (P-III-C) bireyler dahil edildi. Gingivitis ve periodontitis gruplarına CPT uygulandı. Başlangıçta tüm bireylerin, CPT sonrası 1. ve 3. aylarda tedavi gruplarının periodontal parametreler ölçülüp tükürük ile serum örnekleri toplandı. MMP-8, M-CSF, MIP-1α, MAF, IL-34 seviyeleri ELISA yöntemiyle analiz edildi. Bulgular: Tüm klinik parametreler CPT sonrasında tüm gruplarda anlamlı olarak iyileşme gösterdi (p<0,05). Başlangıçta tükürük MMP-8 seviyesi, sağlıklı gruba göre diğer gruplarda daha yüksek tespit edildi (p<0,05). P-III-C grubunda P-III-B grubuna göre başlangıçta daha yüksek olan tükürük MMP-8 seviyesi (p=0,044), iki periodontitis grubunda CPT sonrası tüm dönemlerde düştü (p<0,05). Tükürük MAF seviyesi başlangıçta P-III-B ve P-III-C gruplarında, sağlıklı gruba göre daha yüksek tespit edildi (p<0,05) ve bu gruplarda CPT sonrası 1. ve 3. aylarda başlangıca göre düşüş görüldü (p<0,05). Tükürük IL-34 seviyesi sağlıklıya göre gingivitis ve P-III-C grubunda daha yüksekti (p<0,05). Sonuçlar: Tükürük MMP-8 ve MAF, P-III-B ve P-III-C ile ilişkili, tükürük IL-34 ise gingivitis ve P-III-C ile ilişkili tespit edildi. Periodontitiste MMP-8 ile MAF’ın periodontal tedavinin takibinde kullanılabileceği ortaya koyuldu.
Objective: This study aimed to compare the levels of matrix metalloproteinase-8 (MMP-8), macrophage colony-stimulating factor (M-CSF), macrophage inflammatory protein-1α (MIP-1α), macrophage-activating factors (MAF), interleukin-34 (IL-34) in saliva and serum of different periodontal conditions and to evaluate the effect of non-surgical periodontal treatment (NSPT). Material and Methods: A totally 85 individuals consisting of 20 periodontally healthy, 20 gingivitis, 20 periodontitis stage III grade B (P-III-B) and 25 periodontitis stage III grade C (P-III-C) were recruited to the study. NSPT was performed for gingivitis and periodontitis patients. The periodontal parameters were recorded, saliva and serum samples were obtained from all participants at baseline. In treatment groups, the periodontal parameters were reevaluated, and the samples were recollected at 1 and 3 months following the NSPT. MIP-1α, MAF, MMP-8, M-CSF, IL-34 levels were analyzed by ELISA. Results: All clinical parameters improved in all time points in all treatment groups (p<0,05). At baseline, salivary MMP-8 levels were higher in treatment groups than the healthy group (p<0,05). Salivary MMP-8 levels, higher at baseline in P-III-C group than P-III-B group (p=0,044) and decreased in all time points (p<0,05). Salivary MAF levels were lower in healthy group than P-III-B and P-III-C groups (p<0,05), and decreased at 1st and 3rd months (p<0,05). Salivary IL-34 levels were higher in gingivitis and P-III-C groups than healthy controls (p<0,05). Conclusion: High salivary MMP-8 and MAF levels were associated with P-III-B and P-III-C and salivary IL-34 levels with gingivitis and P-III-C. NSPT was remarkably reduced salivary MMP-8 and MAF levels in periodontitis groups.
Objective: This study aimed to compare the levels of matrix metalloproteinase-8 (MMP-8), macrophage colony-stimulating factor (M-CSF), macrophage inflammatory protein-1α (MIP-1α), macrophage-activating factors (MAF), interleukin-34 (IL-34) in saliva and serum of different periodontal conditions and to evaluate the effect of non-surgical periodontal treatment (NSPT). Material and Methods: A totally 85 individuals consisting of 20 periodontally healthy, 20 gingivitis, 20 periodontitis stage III grade B (P-III-B) and 25 periodontitis stage III grade C (P-III-C) were recruited to the study. NSPT was performed for gingivitis and periodontitis patients. The periodontal parameters were recorded, saliva and serum samples were obtained from all participants at baseline. In treatment groups, the periodontal parameters were reevaluated, and the samples were recollected at 1 and 3 months following the NSPT. MIP-1α, MAF, MMP-8, M-CSF, IL-34 levels were analyzed by ELISA. Results: All clinical parameters improved in all time points in all treatment groups (p<0,05). At baseline, salivary MMP-8 levels were higher in treatment groups than the healthy group (p<0,05). Salivary MMP-8 levels, higher at baseline in P-III-C group than P-III-B group (p=0,044) and decreased in all time points (p<0,05). Salivary MAF levels were lower in healthy group than P-III-B and P-III-C groups (p<0,05), and decreased at 1st and 3rd months (p<0,05). Salivary IL-34 levels were higher in gingivitis and P-III-C groups than healthy controls (p<0,05). Conclusion: High salivary MMP-8 and MAF levels were associated with P-III-B and P-III-C and salivary IL-34 levels with gingivitis and P-III-C. NSPT was remarkably reduced salivary MMP-8 and MAF levels in periodontitis groups.