Publication: Subaraknoid Kanama Sonrası Gastrointestinal ve Hepatik Sistemde Gelişenİnflamatuar Yanıt Üzerinde Hiperbarik Oksijen Tedavisinin Etkileri: Deneysel Çalışma
Abstract
Giriş: Bu çalışmada deneysel SAK modelinde gelişebilecek gastointestinal inflamatuar yanıt ve histopatolojik değişiklikler üzerinde hiperbarik oksijen tedavisinin azaltıcı etkisi araştırıldı. Materyal ve Metod: 20 adet tavşan randomize 4 gruba ayrıldı. Grup I deki deneklere herhangi bir işlem uygulanmadı ve kontrol grubu olarak alındı . Grup II deki deneklere tedavi uygulanmayıp sham grubu olarak alındı. III.grupta SAK oluşturuldu. IV.grupta SAK oluşturulduktan sonra toplam 5 kez HBOT uygulandı. İşlemden 72 saat sonra çalışmaya alınan tüm hayvanlara perfüzyon-fiksasyon yöntemi ile ötanazi uygulandı. Karaciğer ve terminal ileum alındıktan sonra 4 mm'lik kesitler elde edilerek dokular mikroskopik olarak incelendi. Yine her bir dokudan randomize alınan 5 farklı örneğin ışık mikroskopisi altında arteriolar çapları ölçüldü. Tüm sonuçlar Mann- Whitney U testi kullanılarak istatiksel olarak analiz edildi. Bulgular: SAK grubunda kontrol grubuna göre karaciğer ve ileumda inflamasyon artışı anlamlı idi. HBOT grubu ile karşılaştırıldığında ise anlamlı inflamasyon değişikliği gözlemlenmedi. Gruplar arasında ödemde anlamlı farklılık izlenmedi. Sonuç: Çalışmamızda SAK sonrası karaciğer ve ileum dokusunda gelişen inflamasyonun uygulanan HBOT ile gerilemediği gösterildi.
Objective (Background): To investigate whether experimental subarachnoid hemorrhage (SAH) could induce histopathologic changes and inflammatory response in the gastrointestinal system, and if this response may be attenuated by the potential effects of hyperbaric oxygen therapy (HBOT). Methods: Twenty rabbits were randomly divided into four groups. Group I was not subjected to SAH or sham operation. Group II were subjected to a sham operation. Group III were subjected to SAH and did not receive HBOT after SAH. Group IV were subjected to SAH and received five sessions of HBOT. Animals were euthanized through perfusion and fixation 72 h after the procedures. Four-millimeter tissue sections were obtained from the liver and terminal ileum and were examined microscopically. Arteriolar diameters were measured under light microscopy in five different examples taken randomly from each tissue. Statistical comparisons were performed using the Mann- Whitney U test. Results: Increased inflammation was seen in the liver and terminal ileum in the SAH group compared with the sham and control groups. Inflammation in the SAH group was high compared with the HBOT group, but it did not reach significance. There were no differences between groups regarding edema. Conclusions: We showed that HBOT performed after induced SAH was ineffective in the attenuation of inflammation in the liver and ileum tissues that occurred after SAH.
Objective (Background): To investigate whether experimental subarachnoid hemorrhage (SAH) could induce histopathologic changes and inflammatory response in the gastrointestinal system, and if this response may be attenuated by the potential effects of hyperbaric oxygen therapy (HBOT). Methods: Twenty rabbits were randomly divided into four groups. Group I was not subjected to SAH or sham operation. Group II were subjected to a sham operation. Group III were subjected to SAH and did not receive HBOT after SAH. Group IV were subjected to SAH and received five sessions of HBOT. Animals were euthanized through perfusion and fixation 72 h after the procedures. Four-millimeter tissue sections were obtained from the liver and terminal ileum and were examined microscopically. Arteriolar diameters were measured under light microscopy in five different examples taken randomly from each tissue. Statistical comparisons were performed using the Mann- Whitney U test. Results: Increased inflammation was seen in the liver and terminal ileum in the SAH group compared with the sham and control groups. Inflammation in the SAH group was high compared with the HBOT group, but it did not reach significance. There were no differences between groups regarding edema. Conclusions: We showed that HBOT performed after induced SAH was ineffective in the attenuation of inflammation in the liver and ileum tissues that occurred after SAH.