Publication: Retrotranspozon temelli moleküler belirteçler kullanılarak Türk arpa (Hordeum vulgare L.) çeşitlerinin genomik karakterizasyonu
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Kökeni Doğu Akdeniz ülkelerine dayanan, hayvan yemi ve maltlık olarak tüketilen arpanın,dünyanın birçok bölgesinde tarımı ve ıslahı yapılmaktadır. Arpa çeşitlerinde genetikçeşitliliğin belirlenmesi, çeşitler arasındaki genetik ilişkilerin ortaya çıkarılması ve ıslahçıhaklarının korunması için moleküler yöntemlerden faydalanılmaktadır. Bu çalışmada, arpagenomunun önemli bileşenlerinden olan BARE-1 retrotranspozonu ile ilişkili IRAP(Retrotranspozon-arası Çoğaltılmış Polimorfizm), REMAP (Retrotranspozon-MikrosatellitÇoğaltılmış Polimorfizmi) ve iPBS (Primer Arası Bağlanma Yeri Polimorfizmi) olarakadlandırılan moleküler belirteçler kullanılarak ulusal birçok çeşidin karakterizasyonuyapılmıştır. Toplamda 211 alel olmak üzere, 3 IRAP primer çifti 49.5 REMAP primer çifti 55ve 7 iPBS primer çifti için 107 alel tespit edilmiştir. Lokus başına ortalama 14 alelbelirlenmiştir. Polimorfik Bilgi İçeriği (PIC) ortalama değeri REMAP için 0.407, IRAP için0.454 ve IPBS için de 0.442 bulunmuştur. Genetik benzerlik değerleri 0.41 ila 0.93 arasındadeğişmiştir. Tüm belirteçler için ortalama fark yöntemi (UPGMA) kümeleme analiziyapılarak, çeşitler genetik benzerliklerine göre gruplandırılmışlardır. Bu çalışmada, fazlasayıda alel ve yüksek PIC değeri vermeleri, ucuz ve kolay elde edinimleri nedeniyle,transposon temelli moleküler belirteçler ile çok yakın ilişkili çeşitlerin dahi ayrımının kolaybir şekilde yapılabileceği görülmüştür. Transposon esaslı belirteçler, çeşitler arasında genetikilişkileri belirlemenin yanı sıra genetik kaynakların korunmasında ve tohum bankalarınınyönetiminde faydalı olabilecektir.
Barley, which is originated from Eastern Mediterranean countries and is consumed as animal_x000D_ feed and malt, is cultivated and breeding in many regions of the world. Molecular methods are_x000D_ benefited to determine the genetic diversity in barley cultivars, to define the genetic_x000D_ relationship between cultivars and to protect the breeder rights. In this study, characterization_x000D_ of many national cultivars was carried out using molecular markers called as IRAP (Inter_x000D_ retrotransposon amplified polymorphism), REMAP (Retrotransposon microsatellite_x000D_ polymorphism) and iPBS (inter-priming binding site) associated with BARE-1_x000D_ retrotransposons that is one of the important components of barley genome. A total of 211_x000D_ alleles, 49 for 3 IRAP, 55 for 5 REMAP, and 107 for 7 IPBS primer pairs were detected. An_x000D_ average of 14 alleles per locus was determined. Polymorphic Information Content (PIC) mean_x000D_ value was 0.407 for REMAP and 0.454 for IRAP and 0.442 for IPBS. Genetic similarity_x000D_ values ranged from 0.41 to 0.93. Cultivars were divided into groups according to their genetic_x000D_ similarities by performing the unweighted pair group method with arithmetic mean (UPGMA)_x000D_ clustering analysis for all markers. In this study, it was observed that, due to their high number_x000D_ of alleles and high PIC values, and their cheap and easy acquisition, it can be easily_x000D_ distinguished even very closely related cultivars by using transposon-based molecular_x000D_ markers. Transposon based markers can be useful for conservating the genetic resources and_x000D_ managing the seed banks beside as determining the genetic relationship between cultivars.
Barley, which is originated from Eastern Mediterranean countries and is consumed as animal_x000D_ feed and malt, is cultivated and breeding in many regions of the world. Molecular methods are_x000D_ benefited to determine the genetic diversity in barley cultivars, to define the genetic_x000D_ relationship between cultivars and to protect the breeder rights. In this study, characterization_x000D_ of many national cultivars was carried out using molecular markers called as IRAP (Inter_x000D_ retrotransposon amplified polymorphism), REMAP (Retrotransposon microsatellite_x000D_ polymorphism) and iPBS (inter-priming binding site) associated with BARE-1_x000D_ retrotransposons that is one of the important components of barley genome. A total of 211_x000D_ alleles, 49 for 3 IRAP, 55 for 5 REMAP, and 107 for 7 IPBS primer pairs were detected. An_x000D_ average of 14 alleles per locus was determined. Polymorphic Information Content (PIC) mean_x000D_ value was 0.407 for REMAP and 0.454 for IRAP and 0.442 for IPBS. Genetic similarity_x000D_ values ranged from 0.41 to 0.93. Cultivars were divided into groups according to their genetic_x000D_ similarities by performing the unweighted pair group method with arithmetic mean (UPGMA)_x000D_ clustering analysis for all markers. In this study, it was observed that, due to their high number_x000D_ of alleles and high PIC values, and their cheap and easy acquisition, it can be easily_x000D_ distinguished even very closely related cultivars by using transposon-based molecular_x000D_ markers. Transposon based markers can be useful for conservating the genetic resources and_x000D_ managing the seed banks beside as determining the genetic relationship between cultivars.