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Comparison of the solubilization of human platelet protein, cholesterol, gamma glutamyltransferase and lactate dehydrogenase by triton X-100 and Brij-35

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dc.contributor.authorsŞener A., Yardimci T., Uras A.R.
dc.date.accessioned2022-03-28T14:51:40Z
dc.date.accessioned2026-01-11T15:23:01Z
dc.date.available2022-03-28T14:51:40Z
dc.date.issued2003
dc.description.abstractTriton X-100 and Brij 35 are nonionic polyoxyethylene based detergents. Compared with Brij 35, Triton X-100 is used more frequently for the solubilization of proteins and lipids. In this study, the solubilization of human platelet protein, cholesterol, gamma glutamyltransferase and lactate dehydrogenase in both detergents were compared. Platelets were treated with Triton X-100 and Brij 35 at various concentrations and various time periods. No significant differences were observed between the detergents at various concentrations and incubation times in terms of the solubilization of platelet protein, cholesterol, gamma glutamyltransferase or lactate dehydrogenase.
dc.identifier.issn1300638X
dc.identifier.urihttps://hdl.handle.net/11424/255724
dc.language.isoeng
dc.relation.ispartofActa Pharmaceutica Turcica
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBrij 35
dc.subjectPlatelets
dc.subjectProtein solubility
dc.subjectTriton X-100
dc.titleComparison of the solubilization of human platelet protein, cholesterol, gamma glutamyltransferase and lactate dehydrogenase by triton X-100 and Brij-35
dc.typearticle
dspace.entity.typePublication
oaire.citation.endPage152
oaire.citation.issue2
oaire.citation.startPage147
oaire.citation.titleActa Pharmaceutica Turcica
oaire.citation.volume45

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