Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Open Access The effects of Origanum onites in streptozotocin-induced diabetes mellitus in rats(2022-01-01) POLAT, ELİF BEYZANUR; TAŞKIN, TURGUT; BİTİŞ, LEYLA; ELÇİOĞLU, HATİCE KÜBRA; Aydemir O., Polat E. B. , Aljesri K., TAŞKIN T., BİTİŞ L., ELÇİOĞLU H. K.© 2022 Marmara University Press.Diabetes mellitus is a chronic disease characterized by decreased insulin synthesis and/or increased blood glucose due to insulin resistance. In this study, streptozotocin (STZ)-induced diabetic rats were evaluated and it was aimed to investigate the protective effects of Origanum onites L. (OO) against possible changes in these parameters. Male Sprague-Dawley rats weighing 300-400g were divided into three groups control (C), diabetes mellitus (DM) and diabetes mellitus + OO (OO group). DM was induced by administration of STZ 60 mg/kg dose intraperitoneally (i.p.) after 48 hours, rats with blood glucose values higher than 200 mg/dL were considered DM. Origanum extract was administered i.p. to the OO group at 50 mg/kg per day for 6 weeks. Serum AST, ALT, creatinine, and inflammatory cytokine levels were measured. MDA and GSH levels were measured in liver tissues. AST, ALT, creatinine, and MDA levels were found to be increased in the DM group, while a significant decrease in these levels was observed in the treated group. While GSH values fell in the DM group, a significant increase occurred in the OO group (n=6, p<0.0001; two-way ANOVA). When the plasma levels of cytokines were examined, an increase was observed in the DM group and a significant decrease was observed in the OO group. When we evaluate our findings, we think that OO has a protective effect against complications that may occur in DM by preventing oxidant damage and inflammation. Further studies are needed on the protective effects of OO in DM.Publication Open Access Phytochemical screening and biological evaluation of Salvia hydrangea Dc. ex Benth. growing in eastern Anatolia(2022-07-01) TAŞKIN, TURGUT; Toplan G. G., KÜRKÇÜOĞLU M., GÖGER F., TAŞKIN T., Civas A., İŞCAN G., Ecevit-Genc G., Mat A., Baser K. H. C.Salvia species have lately gained significant interest as a result of their suitable utilization in various industries. In the current study, S. hydrangea, one of the most consumed sages in the eastern region of Turkey, has been evaluated for phytochemical composition as well as in vitro pharmacological potential comparatively for the first time. The phytochemical composition of S.hydrangea was investigated by LC-MS/MS, GC-FID, and GC/MS. To reveal its biological activities, antioxidant, antimicrobial, and also acetylcholinesterase activities of different solvent extracts such as water, n-hexane, chloroform, and methanol were determined. According to GC-GC/MS analysis, the primary components of the oil were identified as camphor (46.0%), 1,8-cineole (7.5%), camphene (6.8%), limonene (6.5%), beta-pinene (6.11%) and alpha-pinene (5.6%). Additionally, in the infusion and methanol extract, rosmarinic acid and luteolin glycoside were detected as predominant phenolics by LCMS/MS. In DPPH center dot, CUPRAC, and FRAP test results of the samples indicated strong to moderate antioxidant ability in all samples studied, additionally, among them, the infusion exhibited significant acetylcholine inhibition properties comparable with galanthamine. With regard to antimicrobial activity, all of the tested microorganisms had MIC values ranging from 15 to 2000 mu g/mL. Based on these findings, S. hydrangea may have promising properties for a variety of industrial applications in the pharmaceutical, food, and cosmetic industries. (C) 2022 SAAB. Published by Elsevier B.V. All rights reserved.Publication Open Access Antioxidant, Enzyme Inhibitory and Calcium Oxalate Anti-crystallization Activities of Equisetum telmateia Ehrn.(2020-09-15) TAŞKIN, TURGUT; DOĞAN, AHMET; TAŞKIN T., YILMAZ B. N., DOĞAN A.Equisetum L. is the only genus of the Equisetaceae family, which commonly known as horsetails, in English and atkuyruğu or kırkkilit in Turkish. In traditional medicine, Equisetum telmateia Ehrh. is used in diseases of the urinary system, such as pyelonephritis, prostatic hypertrophy, and cystitis. Besides, this species is known to be used by humans to treat kidney stones or kidney sand. The extracts were obtained from the aerial parts of the E. telmateia using three different extraction methods (maceration, Soxhlet, ultrasonic bath) and their antioxidant (ABTS, CUPRAC), anti-urease and anticholinesterase activities were examined. Also, calcium oxalate anti-crystallization activity of Soxhlet methanol extract showing strong antioxidant activity was determined. Soxhlet methanol extract exhibited stronger ABTS radical scavenging (0.0676 mM Trolox/mg extract) and cupric ion reducing/antioxidant (4.351 mM Trolox/mg extract) activity than other extracts. Soxhlet methanol (65.528%) and maceration methanol (61.965%) extracts showed the strongest anticholinesterase activity. In the anti-urease assay, it was found that Soxhlet petroleum ether extract (15.302%) had the highest anti-urease activity. Furthermore, the data obtained showed that the Soxhlet methanol extract had high efficacy in the nucleation and aggregation phase of calcium oxalate crystals. These results prove that Soxhlet methanol extract has antioxidant, anticholinesterase and anti-crystallization capabilities. Therefore, this extract can be used in the future as an antioxidant and anticholinesterase agent as well as the treatment and / or prevention of stone formation.Publication Open Access Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of Achillea biebersteinii Afan. and A. millefolium subsp. millefolium Afan. L. Growing in Eastern Turkey(2022-03-01) TAŞKIN, TURGUT; Toplan G. G., TAŞKIN T., İŞCAN G., GÖGER F., KÜRKÇÜOĞLU M., Civas A., Ecevit-Genc G., Mat A., Baser K. H. C.The potential applications of Achillea species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biological activities of the aerial parts from Achillea biebersteinii Afan. and Achillea millefolium subsp. millefolium Afan. collected from Turkey were evaluated. Different solvent extracts (n-hexane, chloroform, methanol, water) were prepared and their antimicrobial, anticholinesterase, and antioxidant activities were studied. The LC-MS/MS results revealed the presence of 16 different phenolic compounds, including chlorogenic acid, rutin, quercetin, and luteolin glycosides, in methanolic extracts. According to GC-FID and GC/MS results, the primary components of the oils were identified as 1,8-cineole (32.5%), piperitone (14.4%), and camphor (13.7%) in A. biebersteinii and 1,8-cineole (12.3%) and beta-eudesmol (8.9%) in A. millefolium subsp. millefolium. The infusion and methanolic extracts of both species were found to be rich in their total phenolic content as well as their antioxidant and anticholinesterase activity. In contrast, the n-hexane and chloroform extracts of both species showed strong antimicrobial activity with MIC values ranging from 15 to 2000 mu g/mL. Our findings suggest that the investigated Achillea species could be evaluated as potent natural agents, and further studies into the promising extracts are needed.Publication Open Access Rosa canina L. improves learning and memory-associated cognitive impairment by regulating glucose levels and reducing hippocampal insulin resistance in high-fat diet/streptozotocin-induced diabetic rats(2023-09-15) ERTAŞ, BÜŞRA; YAVUZ, AYŞE NUR; TAŞKIN, TURGUT; ÇAM, MUHAMMET EMİN; ERTAŞ B., YAVUZ A. N., Topal F., Keles-Kaya R., Karakus Ö., Ozcan G. S., TAŞKIN T., ÇAM M. E.Ethnopharmacological relevance: Recent studies claim that Type-2 diabetes mellitus (T2DM) and Alzheimer\"s disease (AD) overlap in several common pathological pathways which from neuronal damage to impaired memory performance. It is known that the use of Rosa canina L. (R. canina) as medicine in folk medicine dates back to ancient times and is used in the treatment of nervous diseases in Persian medicine. However, the effect of R. canina on diabetes-related cognitive decline and memory impairment has not yet been studied. Aim of the study: We evaluated the impact of T2DM on AD-like alterations and examined the molecular mechanism of a possible effect of R. canina on cognitive alterations in diabetic rats. Materials&methods: R. canina ethanol extract was obtained by maceration method. This study was performed with male Sprague-Dawley rats fed with a high-fat diet (HFD) for 8 weeks, low-dose streptozotocin (STZ; 35 mg/kg IP) injection for 4 weeks, and R. canina (250 mg/kg; per oral) and metformin (400 mg/kg; per oral) administration for 4 weeks. The weight and blood glucose of rats were measured weekly. To evaluate glucose tolerance area under the curve (AUC) was calculated by performing an oral glucose tolerance test. Then the rats were subjected to behavioural tests, and their hippocampus and cortex tissues were obtained for biochemical and morphological analyses. Results: R. canina could manage glucose responsiveness by reducing post-prandial blood glucose levels, preventing weight loss, and raising serum insulin levels in T2DM-induced rats. Behavioural tests showed that R. canina significantly improves diabetes-related cognitive decline in recall and long-term memory. Treatment with R. canina significantly reversed HFD/STZ-induced increases in insulin, amyloid-β, amyloid precursor protein levels, and acetylcholinesterase activity in the prefrontal cortex and hippocampus. Furthermore, histological analyzes revealed the protection of R. canina against neuronal disruption in the cortical and hippocampal CA3 region caused by chronic hyperglycemia. Conclusion: Analyzed collectively, these results suggest that R. canina can correct T2DM-related cognitive decline may be attributed to insulin pathway modulation, prevention of amyloid deposition, and increased cholinergic transmission.Publication Open Access Composition characterization and biological activity study of achillea vermicularis extract and extract-loaded nanoparticles(2023-01-01) YILMAZ SOYDAN, NACİYE TUBA; TAŞKIN, TURGUT; DOĞAN M., KOÇYİĞİT Ü. M., Çetin F. N., YILMAZ SOYDAN N. T., TAŞKIN T.© 2023 International Union of Biochemistry and Molecular Biology, Inc.Antiproliferative activity of Achillea vermicularis extracts was calculated on glial (C6) and keratinocyte (HaCaT) cell lines using XTT assay. It was observed that all extracts of A. vermicularis at the determined concentration were not cytotoxic in HaCaT cell lines. The nanoparticles (NPs) of the extract with the best cytotoxic activity was prepared, and necessary characterization studies were performed. Results showed that NP containing the extract has a lower IC50 value and more cytotoxic activity in C6 cells compared to the only extract. Furthermore, the antiepileptic potentials of these substances were explored in this study. The effect of A. vermicularis extracts on the enzyme activities of carbonic anhydrase I and II isoenzymes (hCA I and hCA II) was measured using spectrophotometry to achieve this goal. A. vermicularis extracts demonstrated high inhibitory activities compared to standard inhibitor (acetazolamide, AAZ), with IC50 values in the range of 5.04–10.8 μg/ml for hCA I, and 5.40–9.22 μg/ml for hCA II. High-performance liquid chromatography diode array detector (HPLC-DAD) was used in this investigation to assess the main chemicals found in the extract and NPs. The results showed that the ethanol extract (157.636 μg/mg extract) and NPs (4.631 μg/mg extract) had a significant amount of the 8-hydroxy salvigenin component.Publication Open Access Phytochemical studies of Helichrysum armenium subsp. armenium and its antioxidant, acetylcholinesterase, and antimicrobial activities(2024-01-01) TAŞKIN, TURGUT; Kekeç N., TAŞKIN T., AĞAR D. N., ÖZBEK ÇELİK B., KÜLTÜR Ş., SÜZGEÇ SELÇUK S.Helichrysum armenium subsp. armenium plant was extracted with the Soxhlet apparatus. The obtained extract was fractionated and, respectively, AHB (petroleum ether–60% ethanol-chloroform), AHC (ethanol-toluene), AHD (ethanol-chloroform), and AHE (ethanol-ethylacetate) extracts were obtained. Then, antimicrobial, antioxidant, and acetylcholinesterase activity studies were performed on these extracts. In this study, with the using the LC-HRMS method from the aerial parts of Helichrysum armenium subsp. armenium, the flavonoids: apigenin, apigenin 7-glucoside, apigenin 7-methylate, luteolin 7-glucoside, kaempferol, quercetin, quercitrin, (+)-trans taxifolin, (-)-epicatechin gallate, sinensetin, rutin, 3\"-o-methyl quercetin, hispidulin, chrysin, acacetin, genkwanin, chrysoeriol; the phenolic acids: chlorogenic acid, ascorbic acid, fumaric acid, caffeic acid, salicylic acid, caffeic acid phenethyl ester, emodin, vanilic acid; the alkaloid: caffeine and the saponoside; glycyrrhizic acid were obtained.