Person: TAŞKIN, TURGUT
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TAŞKIN
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TURGUT
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Publication Metadata only Characterization of Phenolic Constituents and Pharmacological Activity of Achillea vermicularis(INDIAN PHARMACEUTICAL ASSOC, 2019) DOĞAN, AHMET; Taskin, T.; Balkan, I. E.; Tankin, D.; Dogan, A.The aim of the present study was to compare for the first time, the antiinflammatory, antioxidant and antiurease activities of 15 different extracts of Achillea vermicularis, which were obtained from three extraction methods using five different solvents. Phenolic contents in chloroform extract showed strong antiinflammatory and antioxidant activity and were analysed by high-performance liquid chromatography with diode-array detection and confirmed by liquid chromatography quadrupole time-of-flight mass spectrometry. The active phenolic compounds in chloroform extracts were identified as chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, naringenin, quercetagetin 3,6-dimethyl ether, and 8-hydroxysalvigenin. All these compounds except caffeic acid were detected for the first time in Achillea vermicularis. These results indicated that chloroform extract of the plant possessed a high potential to be used as a natural source in food and pharmaceutical industry.Publication Open Access Investigation of the Effects of Biological Activity and Steeping Time of Four Different Black Teas Commercially Valuable in Turkey(MARMARA UNIV, INST HEALTH SCIENCES, 2019-12-31) TAŞKIN, TURGUT; Taskin, Turgut; Levent, Muhammed; Sahin, TalipObjective: The aim of this study was to compare the phenolic, tannins contents and biological activities (antioxidant, anticholinesterase) of four different black teas commercially valuable in Turkey. Subsequently, to examine the effect of the steeping time on the chemical contents (phenolic and tannins contents) and biological activities of the samples. Methods: The antioxidant activities of samples were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), cupric reducing antioxidant capacity (CUPRAC) and ferric reducing/antioxidant power (FRAP) methods. The amount of total phenolic and tannins contents contained in the samples was determined using the Folin-Ciocalteu reagent (FCR) method. In addition, the anticholinesterase activities of different samples were evaluated by Ellman method. Results: According to the results obtained, filtered tea bag (FT) sample obtained after 5 min steeping exhibited stronger DPPH radical scavenging activity than other samples. In addition, it was found that powder ceylon tea (PCT) samples obtained after 60 min and 10 min steeping had the highest FRAP and CUPRAC values respectively. In this study, it was also determined that the PCT samples obtained after 5 min, 10 min, 15 min, and 30 min steeping contained higher phenolic and tannins contents than the other samples. In anticholinesterase experiment, powder tea (PT) sample obtained after 5 min steeping was found to have the strongest anticholinesterase activity. Conclusion: As a result, it was determined that the amount of phenolic contents generally increased with the infusion time. It was also found that the antioxidant, anticholinesterase activities and tannins content of the tea samples did not show significant change due to the time of the infusion.Publication Open Access The Influence of Different Extraction Methods/Solvents on Composition, Biological Activities and ADMET Predictions of Phenolics in Tribulus terrestris(INST TECNOLOGIA PARANA, 2021) TAŞKIN, TURGUT; Taskin, Duygu; Yilmaz, Beyza Nur; Taskin, Turgut; Omurtag, Gulden ZehraTribulus terrestris is a plant that has medical importance in treating diseases such as cancer, urinary tract infections, kidney stones, rheumatism, and hypertension. The aim of this study is to examine the effects of extraction methods/solvents on the biological activity and chemical content of the plant and then to determine ADMET predictions of phenolics that were analysed quantitatively using HPLC-DAD in the plant. Maceration methanol (IC50:0.277 mg/mL) and chloroform (0.263 mMFeSO(4)/mg extract) extracts were found to have the strongest DPPH radical scavenging and iron (III) ion reducing activity, respectively. It was determined that Soxhlet methanol (0.0225 mMTE/mg extract) and Soxhlet chloroform (1.360 mMTE/mg extract) extracts exhibited stronger radical cation (ABTS.+) scavenging and cupric ion reducing activity compared to other extracts, respectively. It was found that ultrasonic bath methanol extracts showed the highest anti-urease (21.47%) and calcium oxalate anti-crystallization (71.54%, 80.52%) activity. It was also found that Soxhlet methanol extract (66.763%) has strong acetylcholinesterase enzyme inhibition capacity compared to other extracts. Pyrocatechol, vanillic acid, vanillin, rutin and rosmarinic acid were analysed by HPLC-DAD in the plant. Moreover, it was determined that methanol extracts obtained using soxhlet, ultrasonic bath, and maceration contained the highest amount of rutin, pyrocatechol, and rutin, respectively. ADMET predictions of phenolics show that these compounds are easily absorbed and do not have toxic effects, suggesting that this species may be used as a natural medicinal and nutritional source in the future after detailed analysis tests.Publication Metadata only Characterization of the Biological Activity and Phenolics in Achillea lycaonica(TAYLOR & FRANCIS INC, 2018) RAYAMAN, ERKAN; Taskin, Turgut; Taskin, Duygu; Rayaman, Erkan; Dikpinar, Tugce; Suzgec-Selcuk, Sevda; Arabaci, TuranThe purpose of this study was to comparatively evaluate for the first time the antioxidant, antimicrobial, and antiurease effects of A. lycaonica extracts obtained using three extraction methods and five solvents followed by identification of the phenolic compounds obtained by Soxhlet extraction with ethyl acetate and maceration with chloroform that showed the highest total phenolic and flavonoid contents with antioxidant and antiurease activity. The phenolic compounds were identified to be chlorogenic acid, caffeic acid, rutin, dicaffeoylquinic acid, salicylic acid, luteolin, quercetin, naringenin, apigenin, and 8-hydroxy- salvigenin. Among the solvent extraction procedures, Soxhlet approach was found to have higher recoveries than other approaches. The results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays showed that the 1: 1 ethanol-water Soxhlet, ethanol ultrasonic bath, and ethyl acetate Soxhlet extraction methods had the highest DPPH antioxidant activities. The ethyl acetate Soxhlet extraction method showed the highest 2, 2'-azino-bis(3-ethylbenzthiazoline- 6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) antioxidant activity. In addition, the chloroform maceration extraction method exhibited the highest antiurease activity. Therefore, 1: 1 Soxhlet ethanol-water, ultrasonic bath ethanol, and Soxhlet ethyl acetate extracts may be used in medicine and food production as natural antioxidant sources. In addition, maceration with chloroform may provide a potential antiurease agent.Publication Open Access AN EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, ANTIBIOFILM AND CYTOTOXIC ACTIVITIES OF FIVE VERBASCUM SPECIES IN TURKEY(#N/A, 2018-12-22) TAŞKIN, TURGUT; BURCU SEN-UTSUKARCI, SIBEL DOSLER, TURGUT TASKIN, MAHMOUD ABUDAYYAK, GUL OZHAN, AFIFE MATVerbascum L. species are used for different medicinal purposes in Turkish folk medicine such as diuretic, expectorant, laxative, mucolytic, sedative, sudorific, and wound healer. Five Verbascum species, two of which are used in medicine, were investigated for their antimicrobial, antioxidant and cytotoxic activities. Determination of the total phenolic compounds, DPPH and ABTS methods were preferred for the antioxidant tests. The antimicrobial and antibiofilm activities of the extracts, which were not studied previously, were investigated against pathogenic and potential pathogenic agents. The cytotoxicity was assessed by MTT and LDH tests. This study is very important for finding new therapeutic sources and also new, natural sources for food preservation.Publication Open Access Comparative Studies on Essential Oil and Phenolic Content with In Vitro Antioxidant, Anticholinesterase, Antimicrobial Activities of Achillea biebersteinii Afan. and A. millefolium subsp. millefolium Afan. L. Growing in Eastern Turkey(2022-03-01) TAŞKIN, TURGUT; Toplan G. G., TAŞKIN T., İŞCAN G., GÖGER F., KÜRKÇÜOĞLU M., Civas A., Ecevit-Genc G., Mat A., Baser K. H. C.The potential applications of Achillea species in various industries have encouraged the examination of their phytochemical components along with their biological potential. In the present study, phenolic contents and essential oil compositions together with the in vitro biological activities of the aerial parts from Achillea biebersteinii Afan. and Achillea millefolium subsp. millefolium Afan. collected from Turkey were evaluated. Different solvent extracts (n-hexane, chloroform, methanol, water) were prepared and their antimicrobial, anticholinesterase, and antioxidant activities were studied. The LC-MS/MS results revealed the presence of 16 different phenolic compounds, including chlorogenic acid, rutin, quercetin, and luteolin glycosides, in methanolic extracts. According to GC-FID and GC/MS results, the primary components of the oils were identified as 1,8-cineole (32.5%), piperitone (14.4%), and camphor (13.7%) in A. biebersteinii and 1,8-cineole (12.3%) and beta-eudesmol (8.9%) in A. millefolium subsp. millefolium. The infusion and methanolic extracts of both species were found to be rich in their total phenolic content as well as their antioxidant and anticholinesterase activity. In contrast, the n-hexane and chloroform extracts of both species showed strong antimicrobial activity with MIC values ranging from 15 to 2000 mu g/mL. Our findings suggest that the investigated Achillea species could be evaluated as potent natural agents, and further studies into the promising extracts are needed.Publication Metadata only Antioxidant, Anti-urease Activities and Genotoxic Effects of Terfezia Claveryi Methanol Extracts on Human Lymphocytes(MARMARA UNIV, INST HEALTH SCIENCES, 2018) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Yesil Devecioglu, Tugce; Senkardes, IsmailObjective: Terfezia claveryi is a dark brown color truffle and has been used extensively in human diet in Turkey. Therefore; it is an important issue to determine their biological activities and toxicity. This study aims to investigate the antioxidant and anti-urease effects of T. claveryi methanol extracts and to evaluate the genotoxicity of each extract. Methods: The antioxidant activities of methanol extracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate (ABTS) and ferric reducing/antioxidant power (FRAP) methods. The amount of total phenolic compounds contained in the extracts was determined using the Folin-Ciocalteu reagent (FCR) method. Anti-urease activities of methanol extracts were evaluated by indophenol method. In addition, potential DNA damaging effects of T. claveryi extracts on human lymphocytes were determined by the alkaline comet assay. Results: UM extract exhibited higher DPPH radical scavenging activity than MM extract [the concentration of the substance which provides the inhibition of 50% of the radical (IC50): 0.77 +/- 0.01 mg/mL, 1.17 +/- 0.02 mg/mL, respectively, p<0.0001] however, lower activity than standard (p<0.0001). Both extracts in different concentrations of T. claveryi caused DNA damage (p<0.0001) compared with solvent control DMSO. Also, total comet scores was higher in cells which had been incubated with MM extract (p<0.05). UM extract exhibited higher ferric reducing and anti-urease activity than MM extract but not found statistically different; also activities of both extract were found lower than positive controls of each methods (p<0.0001). Conclusion: T. claveryi methanol extracts induced genotoxicity on lymphocytes and showed very low antioxidant activity compared to standards. This preliminary study should be confirmed with further studies and in different cell lines.Publication Open Access Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts(MARMARA UNIV, 2020-05-13) TAŞKIN, TURGUT; Balkan, Irem Atay; Taskin, Turgut; Sah, Esra Acar; Akaydin, Galip; Yesilada, ErdemDespite widespread traditional usage of Eryngium species in Anatolia (Turkey), only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro anti-inflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH center dot, ABTS center dot+ and CUPRAC assays. The EtOH extract of E. kotschyi roots (EKr EtOH) and aerial parts (EKae EtOH) inhibited 50.08% and 41.52% of NO production at 100 mu g/ml, respectively. The EtOH extract of the roots of E. campestre var. virens (ECr EtOH) and EKr EtOH provided 36.22% and 65.23% IL-6 inhibition and 44.24% and 56.84% IL-1a inhibition at 100 mu g/ml. EKae EtOH exerted highest antioxidant activity on ABTS center dot+ (2.4 +/- 0.0005 mu M trolox/mg extract) and CUPRAC (0.97 +/- 0.07 mM trolox/mg extract). This extract was also found the richest among all in terms of phenolics content (6.1 +/- 0.001 mg/GAE/g extract). EKr EtOH and ECr EtOH exhibited strongest DPPH center dot (IC50 = 1.132 +/- 0.057 mg/ml) radical scavenging and ferric reducing/antioxidant power (0.36 +/- 0.005 mM Fe2+/mg extract) activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.Publication Metadata only Investigation of the Biological Activities of Different Extracts from Dipsacus laciniatus Aerial(BENTHAM SCIENCE PUBL, 2020) ŞENKARDEŞ, İSMAİL; Taskin, Turgut; Akkilic, Yeliz; Celik, Berna Ozbek; Senkardes, Ismail; Ozakpinar, Ozlem B.Background: The aerial parts of Dipsacus laciniatus are traditionally used in the treatment of colds as cold tea; decoction prepared from its roots and aerial parts are used for the treatment of diabetes and cancer, respectively. The extraction procedures and solvents are important steps in the processing of bioactive constituents from the plant materials. Objective: The aim of this study was to evaluate in vitro antioxidant, antimicrobial, anticholinesterase and cytotoxic activities for the first time in plant's different extracts. Methods: The antioxidant activities of extracts were examined using FRAP and CUPRAC methods, including total phenolic and tlavonoids contents. In addition, the antimicrobial, anticholinesterase and cytotoxic activities of extracts were investigated using mnicrodilution, Ellman and MTT methods, respectively. Results: In the present study, methanol extract showed stronger iron (III) ion reduction (FRAP) and copper (II) ion reducing antioxidant capacity (('UPRAC) than other extracts and also contained higher total phenolic and flavonoid compounds. It was also found that there was a linear relationship between antioxidant activity and phenolic compounds in this study. It was demonstrated that the chloroform and methanol extracts showed moderate antimicrobial activity against S. epidermidis and C. albicatis strains. The chloroform extract had a strong and selective cytotoxic activity for the MCF-7 and HT-29 cell lines, while the methanol extract showed low cytotoxic activity on the same cell lines. In addition, according to the obtained data, chloroform extract showed a toxic effect on normal cell line (NIH/3T3), but other extracts showed no toxic effect. Conclusion: Since methanol extract has no toxic effect on normal cell line and has strong antioxidant, anticholinesterase and antimicrobial activity, it is thought that this extract may be useful as an antioxidant, anticholinesterase and antimicrobial agents in the future.Publication Open Access Phytochemical studies of Helichrysum armenium subsp. armenium and its antioxidant, acetylcholinesterase, and antimicrobial activities(2024-01-01) TAŞKIN, TURGUT; Kekeç N., TAŞKIN T., AĞAR D. N., ÖZBEK ÇELİK B., KÜLTÜR Ş., SÜZGEÇ SELÇUK S.Helichrysum armenium subsp. armenium plant was extracted with the Soxhlet apparatus. The obtained extract was fractionated and, respectively, AHB (petroleum ether–60% ethanol-chloroform), AHC (ethanol-toluene), AHD (ethanol-chloroform), and AHE (ethanol-ethylacetate) extracts were obtained. Then, antimicrobial, antioxidant, and acetylcholinesterase activity studies were performed on these extracts. In this study, with the using the LC-HRMS method from the aerial parts of Helichrysum armenium subsp. armenium, the flavonoids: apigenin, apigenin 7-glucoside, apigenin 7-methylate, luteolin 7-glucoside, kaempferol, quercetin, quercitrin, (+)-trans taxifolin, (-)-epicatechin gallate, sinensetin, rutin, 3\"-o-methyl quercetin, hispidulin, chrysin, acacetin, genkwanin, chrysoeriol; the phenolic acids: chlorogenic acid, ascorbic acid, fumaric acid, caffeic acid, salicylic acid, caffeic acid phenethyl ester, emodin, vanilic acid; the alkaloid: caffeine and the saponoside; glycyrrhizic acid were obtained.