Person: GÜÇLÜ, HÜLYA
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GÜÇLÜ
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HÜLYA
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Publication Metadata only The effect of carnitine against acrylamide-induced toxicity in rat lung and liver tissues(ELSEVIER IRELAND LTD, 2016) GÜÇLÜ, HÜLYA; Aydemir, S.; Guclu, H.; Ozkan, N.; Yuksel, M.; Erdogan, N.; Omurtag, G. Z.Publication Metadata only The protective role of UMCA on bleomycin-induced lung fibrosis in rats(2022-11-05) AYDEMİR, SEZGİN; ÖZKAN YENAL, NAZİYE; GÜÇLÜ, HÜLYA; YÜKSEL, MERAL; ÖZBEYLİ, DİLEK; AYDEMİR S., ÖZKAN YENAL N., GÜÇLÜ H., YÜKSEL M., ÖZBEYLİ D., ERDOĞAN N.Bleomycin (BLM), an antibiotic drug, is used for the treatment of cancers like Hodgkin’s lymphoma, Kaposi’s sarcoma and cervical cancer. The most common adverse effects occurred during BLM treatment is lung toxicity, which manifest as fibrosis. UMCA® (Pelargonium sidoides root extract) is a commercial product to treat acute and chronic upper respiratory tract infections. UMCA® has antiviral, antibacterial, immunomodulatory and cytoprotective properties, which is related to its polyphenol, coumarin, anthocyanidin and flavonoid contents. In the present study, we aimed to investigate the possible protective effects of UMCA® on BLM induced lung fibrosis in rats. Wistar albino rats was included in this study and randomly divided into 4 groups (Control, BLM, BLM+UMCA and UMCA). The control group received physiological saline. UMCA® was orally applied at a dose of 200 mg/kg/day and single intratracheal administration of BLM was conducted at a dose of 10 mg/kg. All animals were decapitated after 10 days and lung tissues was removed for the biochemical and histopathological examinations. Malondialdehyde (MDA) and glutathione (GSH) levels and Myeloperoxidase (MPO) activity were determined. Chemiluminescence (CL) method using luminol and lucigenin probes, additional nitric oxide and peroxynitrite measurements were applied. Histopathological observations were analyzed with H&E and Gomori's one-step trichrome staining. Collagen contents of lung tissue were also determined with a spectrophotometric method in paraffin-embedded tissues. BLM elevated MDA levels and MPO activity and depleted GSH levels in the lung tissues (p<0.001). CL measurement levels were also increased in BLM group respect to the control. Contrary to this, UMCA® treatment reversed these effects, significantly (p<0.001). BLM caused alveolar structural disturbance and inflammatory cell infiltrations and collagen content was also significantly increased in BLM group compared to the control (65.7±10.6, p<0.001). UMCA® administration reduced degenerations and decreased collagen content in the lung tissue. In conclusion, UMCA® has an antioxidant and protective effects on BLM induced lung fibrosis in rats.Publication Open Access Publication Open Access Punica Granatum Peel Extract Protects Against Ionizing Radiation-Induced Enteritis And Leukocyte Apoptosis In Rats(OXFORD UNIV PRESS, 2009) YEGEN, BERRAK; Toklu, Hale Z.; Sehirli, Oezer; Ozyurt, Hazan; Mayadagli, A. Alpaslan; Eksioglu-Demiralp, Emel; Cetinel, Sule; Sahin, Huelya; Yegen, Berrak C.; Dumlu, Melek Ulusoylu; Gokmen, Vural; Sener, GoekselApoptosis/Cytokine/Enteritis/Ileum/Irradiation/Punica granatum/Pomegranate/Radioprotective. Radiation-induced enteritis is a well-recognized sequel of therapeutic irradiation. Therefore we examined the radioprotective properties of Punica granatum peel extract (PPE) on the oxidative damage in the ileum. Rats were exposed to a single whole-body X-ray irradiation of 800 cGy. Irradiated rats were pretreated orally with saline or PPE (50 mg/kg/day) for 10 days before irradiation and the following 10 days, while control rats received saline or PPE but no irradiation. Then plasma and ileum samples were obtained. Irradiation caused a decrease in glutathione and total antioxidant capacity, which was accompanied by increases in malondialdehyde levels, myeloperoxidase activity, collagen content of the tissue with a concomitant increase 8-hydroxy-2'-deoxyguanosine (an index of oxidative DNA damage). Similarly, pro-inflammatory cytokines (TNF-alpha, IL-1 beta and IL-6) and lactate dehydrogenase were elevated in irradiated groups as compared to control. PPE treatment reversed all these biochemical indices, as well as histopathological alterations induced by irradiation. Furthermore, flow cytometric measurements revealed that leukocyte apoptosis and cell death were increased in irradiated animals, while PPE reversed these effects. PPE supplementation reduced oxidative damage in the ileal tissues, probably by a mechanism that is associated with the decreased production of reactive oxygen metabolites and enhancement of antioxidant mechanisms. Adjuvant therapy of PPE may have a potential to support a Successful radiotherapy by protecting against radiation-induced enteritis.