Publication: TQ-Ox, a novel synthetic derivative of thymoquinone on ovarian cancer cells in vitro
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Date
2022-01-01
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Abstract
© 2022 Informa UK Limited, trading as Taylor & Francis Group.There are many studies in the literature on thymoquinone (TQ)-related cancer cells and models, and there is no relevant study investigating the efficacy of the oxime derivative of TQ (TQ-Ox). This study synthesized TQ-Ox and examined its cytotoxic, genotoxic and apoptotic properties in ovarian cancer cells. The structure TQ-Ox was confirmed with NMR. The cytotoxicity by luminometric ATP, intracellular reactive oxygen species (iROS) by fluorometric, intracellular calcium (iCa2+) by fluorometric, mitochondrial membrane potential (MMP) by flow cytometry, glutathione (GSH) levels with GSH/GSSG-Glo assay, DNA damage by comet assay, and apoptosis by acridine orange/ethidium bromide dye were determined. Concentrations of TQ-Ox were statistically increased cytotoxicity, DNA damage, apoptosis, iROS, and iCa2+ in a concentration-dependent manner (p < 0.001). Besides, MMP and GSH levels also decreased statistically significantly (p < 0.001) with increasing concentrations. TQ-Ox would be an effective treatment option by increasing cytotoxicity, genotoxicity, and apoptosis in ovarian carcinoma.
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Tarımsal Bilimler, Ziraat, Bitki Koruma, Fitopatoloji, Yaşam Bilimleri, Moleküler Biyoloji ve Genetik, Sitogenetik, Kimya, Analitik Kimya, Biyokimya, Biyoinorganik Kimya, Temel Bilimler, Agricultural Sciences, Agriculture, Plant Protection, Phytopathology, Life Sciences, Molecular Biology and Genetics, Cytogenetic, Chemistry, Analytical Chemistry, Biochemistry, Bioinorganic Chemistry, Natural Sciences, Tarım ve Çevre Bilimleri (AGE), Temel Bilimler (SCI), Yaşam Bilimleri (LIFE), Bitki ve Hayvan Bilimleri, BİTKİ BİLİMLERİ, KİMYA, ANALİTİK, KİMYA, ORGANİK, BİYOKİMYA VE MOLEKÜLER BİYOLOJİ, Agriculture & Environment Sciences (AGE), Natural Sciences (SCI), Life Sciences (LIFE), PLANT & ANIMAL SCIENCE, CHEMISTRY, MOLECULAR BIOLOGY & GENETICS, PLANT SCIENCES, CHEMISTRY, ANALYTICAL, CHEMISTRY, ORGANIC, BIOCHEMISTRY & MOLECULAR BIOLOGY, Fizik Bilimleri, Bitki Bilimi, Organik Kimya, Physical Sciences, Plant Science, Organic Chemistry, Apoptosis, genotoxicity, ovarian cancer, thymoquinone-oxime
Citation
Kale E., Kale A., Bozali K., Gulgec A. S. , ÖZDEMİR M., YALÇIN B., Guler E. M. , "TQ-Ox, a novel synthetic derivative of thymoquinone on ovarian cancer cells in vitro", Natural Product Research, 2022