Publication:
Evaluation of dendrite morphology in wistar and genetic absence epileptic (GAERS) rats

Simple item page
dc.contributor.authorŞEHİRLİ, ÜMİT SÜLEYMAN
dc.contributor.authorKİRAZLI, ÖZLEM
dc.contributor.authorGÜLHAN, REZZAN
dc.contributor.authorsYAZI S., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F., KİRAZLI Ö.
dc.date.accessioned2023-01-18T11:41:48Z
dc.date.available2023-01-18T11:41:48Z
dc.date.issued2022-07-09
dc.description.abstractAims The aim of the study is to examine the morphological features of dendrites and dendritic spines of pyramidal neurons in somatosensory cortex and hippocampus of Wistar and GAERS (Genetic Absence Epilepsy Rat from Strasbourg) rats. Methods GAERS (n=5) and Wistar (n=5) rats were sacrificed by transcardial perfusion method. Brain tissues were stained using the FD Rapid GolgiStain Kit. Coronal sections of 200 µm thickness were obtained with cryostat. Pyramidal neurons in deep layers of the somatosensory cortex and CA1 region of the hippocampus were examined using light microscope and Neurolucida 360 software. Branching and length of apical dendrite and basal dendrites, and types and densities of dendritic spines were analyzed. Results Statistically the total number of dendrite nodes (p=0.0053, p=0,0047), the total number of dendrite segments (dendritic arborization) (p=0.0036, p=0,0036), the total number of dendrite terminations (p=0.0033, p=0,0029), the total dendrite length (µm) (p=0.0002, p=0,0007) and the dendritic spine density (1/µm) (p=0.0168, p=0,0120) of the somatosensory cortex and the hippocampus were significantly higher in GAERS rats, respectively. When dendritic spine types were evaluated separately, stubby type dendritic spines in the hippocampus were found to be significantly higher in GAERS rats compared to Wistar rats (p=0.0204). Conclusions It was concluded that intense synaptic activation seen in postsynaptic cells in the somatosensory cortex and hippocampus in GAERS causes changes in the dendrite morphology of pyramidal neurons. This study was supported by Marmara University Scientifical Research Projects Commission (TYL-2021-10244).
dc.identifier.citationYAZI S., ŞEHİRLİ Ü. S., GÜLHAN R., ONAT F., KİRAZLI Ö., \"Evaluation of Dendrite Morphology in Wistar and Genetic Absence Epileptic (GAERS) Rats \", Federation of European Neuroscience Congress, Paris, Fransa, 09 Temmuz 2022
dc.identifier.urihttps://forum.fens.org/abstract-e-book/
dc.identifier.urihttps://hdl.handle.net/11424/285428
dc.language.isoeng
dc.relation.ispartofFederation of European Neuroscience Congress
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAbsence epilepsy
dc.subjectGAERS
dc.subjectdendrite
dc.subjectdendritic spine
dc.titleEvaluation of dendrite morphology in wistar and genetic absence epileptic (GAERS) rats
dc.typeconferenceObject
dspace.entity.typePublication
local.avesis.id104e6a76-c1f1-4547-91ae-0eb1a908ead6
relation.isAuthorOfPublication947be220-92d1-428d-bc90-7806465712dd
relation.isAuthorOfPublicatione7204871-8a8b-4411-96e0-3ce3e2a99d12
relation.isAuthorOfPublicationc44fef14-869d-4c28-a766-5e080b17c687
relation.isAuthorOfPublication.latestForDiscovery947be220-92d1-428d-bc90-7806465712dd

Files

Collections

Research Outputs