Publication:
Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres

Simple item page
dc.contributor.authorERASLAN, MUHSİN
dc.contributor.authorÇERMAN, EREN
dc.contributor.authorBOZKURT, SÜHEYLA
dc.contributor.authorAKKOÇ, TUNÇ
dc.contributor.authorsERASLAN M., ÇERMAN E., BOZKURT S., Genç D., Virlan A. T., Demir C. S., Akkoç T., Karaöz E., AKKOÇ T.
dc.date.accessioned2023-09-12T13:21:58Z
dc.date.available2023-09-12T13:21:58Z
dc.date.issued2023-10-01
dc.description.abstractAim: The study aimed to evaluate the differentiation ability of intravitreally injected rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to retinal ganglion-like cells in a polystyrene microsphere induced rat glaucoma model. Materials and Methods: The glaucoma rat model was generated via intracameral injection of 7 microliter polystyrene microspheres. Green fluorescence protein-labeled (GFP) rBM-MSCs were transplanted intravitreally at or after induction of ocular hypertension (OHT), depending on the groups. By the end of the fourth week, flat-mount retinal dissection was performed, and labeled against Brn3a, CD90, GFAP, CD11b, Vimentin, and localization of GFP positive rBM-MSCs was used for evaluation through immunofluorescence staining and to count differentiated retinal cells by flow cytometry. From 34 male Wistar albino rats, 56 eyes were investigated. Results: Flow cytometry revealed significantly increased CD90 and Brn3a positive cells in glaucoma induced and with rBM-MSC injected groups compared to control(P = 0.006 and P = 0.003 respectively), sham-operated (P = 0.007 and P < 0.001 respectively), and only rBM-MSCs injected groups (P = 0.002 and P = 0.009 respectively). Immunofluorescence microscopy revealed differentiation of GFP labeled stem cells to various retinal cells, including ganglion-like cells. rBM-MSCs were observable in ganglion cells, inner and outer nuclear retinal layers in rBM-MSCs injected eyes. Conclusion: Intravitreally transplanted rBM-MSCs differentiated into retinal cells, including ganglion-like cells, which successfully created a glaucoma model damaged with polystyrene microspheres. Promisingly, MSCs may have a role in neuro-protection and neuro-regeneration treatment of glaucoma in the future.
dc.identifier.citationERASLAN M., ÇERMAN E., BOZKURT S., Genç D., Virlan A. T., Demir C. S., Akkoç T., Karaöz E., AKKOÇ T., "Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres", Tissue and Cell, cilt.84, 2023
dc.identifier.doi10.1016/j.tice.2023.102199
dc.identifier.issn0040-8166
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85168726667&origin=inward
dc.identifier.urihttps://hdl.handle.net/11424/293272
dc.identifier.volume84
dc.language.isoeng
dc.relation.ispartofTissue and Cell
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectHistoloji-Embriyoloji
dc.subjectYaşam Bilimleri
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectMikrobiyal Genetik
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectTemel Bilimler
dc.subjectMedicine
dc.subjectHistology and Embryology
dc.subjectLife Sciences
dc.subjectMolecular Biology and Genetics
dc.subjectMicrobial Genetics
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectNatural Sciences
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectGELİŞİMSEL BİYOLOJİ
dc.subjectHÜCRE BİYOLOJİSİ
dc.subjectLife Sciences (LIFE)
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectDEVELOPMENTAL BIOLOGY
dc.subjectCELL BIOLOGY
dc.subjectGelişimsel Biyoloji
dc.subjectHücre Biyolojisi
dc.subjectDevelopmental Biology
dc.subjectCell Biology
dc.subjectFlow cytometry
dc.subjectGlaucoma
dc.subjectMicrobead
dc.subjectNeuro-protection
dc.subjectNeuro-regeneration
dc.subjectPolystyrene microsphere
dc.subjectRetinal ganglion cell
dc.subjectStem cell
dc.titleMesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres
dc.typearticle
dspace.entity.typePublication
local.avesis.id7e535d6a-eb01-490a-96c5-5c685d837237
local.indexed.atPUBMED
local.indexed.atSCOPUS
relation.isAuthorOfPublication22668ace-5f6d-45b8-ab01-b2839b2a5566
relation.isAuthorOfPublication94b7eabe-feca-456f-8edb-62cf4425293a
relation.isAuthorOfPublicationdd039cf9-dfb8-4af0-b879-0b88c3a852fa
relation.isAuthorOfPublicationdde3c531-d82d-492a-96eb-d6724483eaa1
relation.isAuthorOfPublication.latestForDiscovery22668ace-5f6d-45b8-ab01-b2839b2a5566

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
24.pdf
Size:
13.2 MB
Format:
Adobe Portable Document Format
Download

Collections

Research Outputs