Publication: Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres
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Date
2023-10-01
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Abstract
Aim: The study aimed to evaluate the differentiation ability of intravitreally injected rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to retinal ganglion-like cells in a polystyrene microsphere induced rat glaucoma model. Materials and Methods: The glaucoma rat model was generated via intracameral injection of 7 microliter polystyrene microspheres. Green fluorescence protein-labeled (GFP) rBM-MSCs were transplanted intravitreally at or after induction of ocular hypertension (OHT), depending on the groups. By the end of the fourth week, flat-mount retinal dissection was performed, and labeled against Brn3a, CD90, GFAP, CD11b, Vimentin, and localization of GFP positive rBM-MSCs was used for evaluation through immunofluorescence staining and to count differentiated retinal cells by flow cytometry. From 34 male Wistar albino rats, 56 eyes were investigated. Results: Flow cytometry revealed significantly increased CD90 and Brn3a positive cells in glaucoma induced and with rBM-MSC injected groups compared to control(P = 0.006 and P = 0.003 respectively), sham-operated (P = 0.007 and P < 0.001 respectively), and only rBM-MSCs injected groups (P = 0.002 and P = 0.009 respectively). Immunofluorescence microscopy revealed differentiation of GFP labeled stem cells to various retinal cells, including ganglion-like cells. rBM-MSCs were observable in ganglion cells, inner and outer nuclear retinal layers in rBM-MSCs injected eyes. Conclusion: Intravitreally transplanted rBM-MSCs differentiated into retinal cells, including ganglion-like cells, which successfully created a glaucoma model damaged with polystyrene microspheres. Promisingly, MSCs may have a role in neuro-protection and neuro-regeneration treatment of glaucoma in the future.
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Tıp, Histoloji-Embriyoloji, Yaşam Bilimleri, Moleküler Biyoloji ve Genetik, Mikrobiyal Genetik, Sağlık Bilimleri, Temel Tıp Bilimleri, Temel Bilimler, Medicine, Histology and Embryology, Life Sciences, Molecular Biology and Genetics, Microbial Genetics, Health Sciences, Fundamental Medical Sciences, Natural Sciences, Yaşam Bilimleri (LIFE), GELİŞİMSEL BİYOLOJİ, HÜCRE BİYOLOJİSİ, Life Sciences (LIFE), MOLECULAR BIOLOGY & GENETICS, DEVELOPMENTAL BIOLOGY, CELL BIOLOGY, Gelişimsel Biyoloji, Hücre Biyolojisi, Developmental Biology, Cell Biology, Flow cytometry, Glaucoma, Microbead, Neuro-protection, Neuro-regeneration, Polystyrene microsphere, Retinal ganglion cell, Stem cell
Citation
ERASLAN M., ÇERMAN E., BOZKURT S., Genç D., Virlan A. T., Demir C. S., Akkoç T., Karaöz E., AKKOÇ T., "Mesenchymal stem cells differentiate to retinal ganglion-like cells in rat glaucoma model induced by polystyrene microspheres", Tissue and Cell, cilt.84, 2023