Publication: Effect of different immobilization media on breakdown of whey proteins by Streptococcus thermophilus
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2022-05-01
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Abstract
Objective: In this study, we aimed to compare the efficiency of different immobilization media to facilitate breakdown of whey proteins by Streptococcus thermophilus (S. thermophilus). Materials and Methods: S. thermophilus was isolated from yoghurt. High-protein whey powder was present in fermentation media and two-phase dispersion technique was used for immobilization of S. thermophilus in agar, agarose and κ-carrageenan. Total protein after fermentation of whey proteins with S. thermophilus in different media was measured. We have also performed sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis to observe changes in individual whey proteins after fermentation in different media. Results:Total protein concentration showed a significant decrease at the end of 24 hours of fermentation in all media. SDS-PAGE results showed that the amount of both α-lactalbumin and β-lactoglobulin were reduced in all immobilization media compared to control. The effect of κ-carrageenan was considerably higher compared to other media. Conclusion:Our results showed that immobilization in κ-carrageenan increased the breakdown of whey proteins by S. thermophilus and can be used to increase fermentation efficiency
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Tıp, Sağlık Bilimleri, Temel Tıp Bilimleri, Biyokimya, Mikrobiyoloji ve Klinik Mikrobiyoloji, Diğer, Medicine, Health Sciences, Fundamental Medical Sciences, Biochemistry, Microbiology and Clinical Microbiology, Other, Klinik Tıp (MED), Yaşam Bilimleri (LIFE), Klinik Tıp, Biyoloji ve Biyokimya, Mikrobiyoloji, TIP, GENEL & DAHİLİ, MİKROBİYOLOJİ, Clinical Medicine (MED), Life Sciences (LIFE), CLINICAL MEDICINE, BIOLOGY & BIOCHEMISTRY, MICROBIOLOGY, MEDICINE, GENERAL & INTERNAL, İmmünoloji ve Mikrobiyoloji (çeşitli), Aile Sağlığı, Mikrobiyoloji (tıbbi), Temel Bilgi ve Beceriler, Genel Sağlık Meslekleri, Patofizyoloji, Biyokimya (tıbbi), Dahiliye, Değerlendirme ve Teşhis, Tıp (çeşitli), Genel Tıp, Yaşam Bilimleri, Immunology and Microbiology (miscellaneous), Microbiology, Family Practice, Microbiology (medical), Fundamentals and Skills, General Health Professions, Pathophysiology, Biochemistry (medical), Internal Medicine, Assessment and Diagnosis, Medicine (miscellaneous), General Medicine, Life Sciences, alpha-lactalbumin, beta-lactoglobulin, Fermentation, Immobilization, kappa-carrageenan, LACTIC-ACID, LACTOBACILLUS-BULGARICUS, CELLS, CARRAGEENAN, CASEI, α-lactalbumin, β-lactoglobulin, Fermentation, Immobilization, κ-carrageenan
Citation
Safak F. Z., Bıcım G., Yılmaz A. M., Aksu M. B., Yalcın A. S., "Effect of different immobilization media on breakdown of whey proteins by Streptococcus thermophilus", Marmara Medical Journal, cilt.35, sa.2, ss.139-142, 2022