Person: YILMAZ GÖLER, AYŞE MİNE
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YILMAZ GÖLER
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AYŞE MİNE
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Publication Open Access Anti-cancer effects of curcumin, quercetin and tea catechins(MARMARA UNIV, FAC PHARMACY, 2016-09-20) YALÇIN, AHMET SUHA; Yalcin, A. Suha; Yilmaz, Ayse Mine; Altundag, Ergul Mutlu; Kocturk, SemraPolyphenols are present in high amounts in all parts of plants including roots, seeds, flowers, leaves, branches and trunk as well as plant derived products such as tea, coffee and wine. Extensive amount of information is available on biological effects of polyphenols including antioxidant, anti-cancer, anti-inflammatory, anti-coagulant and anti-microbial activities. In recent years, researchers have turned their interest towards identifying molecular mechanisms underlying the anti-cancer effects of these compounds. However, the limited bioavailability of polyphenols and the existence of differences in cancer cells in terms of intracellular mechanisms affected has necessitated the use of specific approaches to individual cancer cell types as well as methods of increasing bioavailability. In this review, the structures, bioavailability, biological activities and molecular mechanisms of anti-cancer effects of curcumin, quercetin and tea catechins are discussed.Publication Metadata only Antitumor and antimetastatic effects of walnut oil in esophageal adenocarcinoma cells(CHURCHILL LIVINGSTONE, 2018) ŞAHİN, ALİ; Batirel, Saime; Yilmaz, Ayse Mine; Sahin, Ali; Perakakis, Nikolaos; Ozer, Nesrin Kartal; Mantzoros, Christos S.Background: Walnuts contain many components including specific fatty acids, which could be active against cancer. Even though the anticarcinogenic effect of some of the individual fatty acids in walnut oil has been described, the effect of walnut oil itself on esophageal cancer cells hasn't yet been investigated. Objective: We aimed to investigate whether walnut oil affects tumor growth and metastatic potential in esophageal cancer cells. Methods: The human esophageal adenocarcinoma cell line, OE19, was treated with different doses of walnut oil and cell viability, apoptosis/necrosis and cell cycle analyses were performed using WST-1 assay and flow cytometry respectively. Adhesion, colony formation and wound healing assays were performed to assess the antimetastatic effects of walnut oil. NFkB expression was evaluated with western blot analysis. Results: Walnut oil decreased the cell viability of esophageal cancer cells in a dose-dependent manner. 20 mg/mL walnut oil reduced cell viability by similar to 50% when compared with control. The analysis revealed that necrosis and accumulation of cells in G0/G1 phase was induced in the cells treated with high doses of walnut oil. It also down-regulated the protein levels of NFkB. Walnut oil suppressed the adhesion, migration and colony formation of the cells. Conclusions: High-dose short-term administration of walnut oil reduces the cell viability and metastatic ability of esophageal cancer cells, while exhibiting anticarcinogenic effect by inducing necrosis and cell cycle arrest at the G0/G1 phase, probably through suppression of the NFkB pathway. These data indicate that walnut oil, and by extension walnut consumption, may have beneficial effects in esophageal cancer in humans. This should be tested by clinical trials in the future. (C) 2017 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.Publication Open Access Cytotoxicity of Different Nano Composite Resins on Human Gingival and Periodontal Ligament Fibroblast Cell Lines: An In Vitro Study(MDPI, 2020-03-01) YILMAZ GÖLER, AYŞE MİNE; Kavuncu, Gamze; Yilmaz, Ayse Mine; Yilmaz, Betul Karademir; Atali, Pinar Yilmaz; Altunok, Elif Cigdem; Kuru, Leyla; Agrali, Omer BirkanThe aim of this study is to determine the cytotoxicity of three different nano composite resins (CRs) on human gingival fibroblast (hGF) and periodontal ligament fibroblast (hPDLF) cell lines. These CRs selected were nanohybrid organic monomer-based Admira Fusion (AF), nanohybrid Bis-(acryloyloxymethyl) tricyclo [5.2.1.0.sup.2,6] decane-based Charisma Topaz (CT), and supra nano filled resin-based Estelite Quick Sigma (EQS). MTT assay was performed to assess the cytotoxicity of CRs at 24 h and one week. AF and EQS applied on hGF cells at 24 h and one week demonstrated similar cytotoxic outcomes. Cytotoxicity of CT on hGF cells at one week was higher than 24 h (p = 0.04). Cytotoxicity of CT on hGF cells was higher at 24 h (p = 0.002) and one week (p = 0.009) compared to control. All composites showed higher cytotoxicity on hPDLF cells at one week than the 24 h (AF; p = 0.02, CT; p = 0.02, EQS; p = 0.04). AF and EQS demonstrated lower cytotoxicity on hPDLF cells than the control group at 24 h (AF; p = 0.01, EQS; p = 0.001). CT was found more cytotoxic on hPDLF cells than the control (p = 0.01) and EQS group (p = 0.008) at one week. The cytotoxicity of CRs on hGF and hPDLF cells vary, according to the type of composites, cell types, and exposure time.Publication Metadata only Comparison of antioxidant capacity, protein profile and carbohydrate content of whey protein fractions(ELSEVIER SCI LTD, 2014) YALÇIN, AHMET SUHA; Onay-Ucar, Evren; Arda, Nazli; Pekmez, Murat; Yilmaz, Ayse Mine; Boke-Sarikahya, Nazli; Kirmizigul, Suheyla; Yalcin, A. SuhaWhey is used as an additive in food industry and a dietary supplement in nutrition. Here we report a comparative analysis of antioxidant potential of whey and its fractions. Fractions were obtained by size exclusion chromatography, before and after enzymatic digestion with pepsin or trypsin. Superoxide radical scavenging, lipid peroxidation inhibition and cupric ion reducing activities of different fractions were checked. Peptides were detected by SDS-PAGE and GC-MS was used to determine carbohydrate content of the fractions. All samples showed antioxidant activity and the second fraction of the trypsin hydrolysate showed the highest superoxide radical scavenging activity. CUPRAC value of this fraction was two-times higher than that of whey filtrate. The first fraction of the pepsin hydrolysate was the most effective inhibitor of lipid peroxidation. Each sample exhibited a different polypeptide profile. Different percentages of carbohydrates were identified in whey filtrate and in all second fractions, where galactose was the major component. (C) 2013 Elsevier Ltd. All rights reserved.Publication Metadata only Combination of proteasome inhibitors with temozolomide to increase the anti-tumor effect in 3D culture model of glioblastoma(ELSEVIER SCIENCE INC, 2018) YILMAZ GÖLER, AYŞE MİNE; Unal, Semra; Gokce, Tilbe; Arslan, Sema; Yilmaz, Ayse Mine; Gunduz, Oguzhan; Karademir, BetulPublication Metadata only Effect of different culture media on isolation and differentiation of dendritic cells(WALTER DE GRUYTER GMBH, 2015) YALÇIN, AHMET SUHA; Yilmaz, Ayse Mine; Altundag, Ergul Mutlu; Gedik, Gulsah; Kocturk, Semra; Yalcin, A. Suha; Taga, YavuzObjective: Dendritic cells (DCs) are members of the mammalian immune system and are considered to be the most powerful antigen presenting cells. They are responsible for the induction of T-cells or T-cell dependent immunity and tolerance. In this study we have investigated the effect of different serum supplements on generation and yield of mature dendritic cells isolated from peripheral blood mononuclear cells. Methods: Three different serum supplements (10% Fetal Bovine Serum, 1% Human Serum Albumin and 1% autologous serum) were compared with serum-free media to identify the role and importance of serum supplements on DC cultivation. Effect of different media on maturation signs (CD40, CD80, CD86, CD209a) and cytokine release (TNF-alpha, IL-10, IL-12, IL-6) was examined. Results: DCs generated in serum-free media was similar to those of cells in medium with autologous serum. Few dendritic-like cells were observed in fetal bovine serum and human serum albumin. The effect of different media on maturation of DCs was compared phenotypically and increased expression of CD80, CD86 and CD209a identified maturation and yield of DCs. Conclusion: Our results suggest that serum free media can be used to overcome potential drawbacks associated with different serum containing supplements.Publication Open Access Klorheksidinin insan dişeti fibroblastları üzerindeki hücre canlılığı ve sitotoksisite etkinliğinin in vitro koşullarda değerlendirilmesi(2023-04-01) YILMAZ GÖLER, AYŞE MİNE; ÖZTÜRK ÖZENER, HAFİZE; Bayraktar G., YILMAZ GÖLER A. M., ÖZTÜRK H.Amaç: Bu in vitro çalışmada, % 0,2’lik klorheksidin (CHX) solüsyonunun, insan dişeti fibroblast (HGF) hücre canlılığı ve sitotoksisitesi üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: Bu çalışma 30 saniye ve 2 dakikalık zaman aralıklarında, nötr pH değerindeki % 0,2’lik CHX solüsyonu ve hücre olarak ATCC’den ticari olarak temin edilen HGF-1 (CRL2014) hücre hatları kullanılarak gerçekleştirildi. CHX’in HGF üzerindeki hücre canlılığı etkileri 3-(4,5-dimetiltiazol-2-il)-2,5- difeniltetrazolyum bromür (MTT) testi ile ve sitotoksik etkileri laktat dehidrogenaz (LDH) testi ile değerlendirildi. Sonuçlar ortalama ve standart sapma değerleri kullanılarak two way ANOVA testiyle istatistiksel olarak analiz edildi (p<0,05). Bulgular: MTT testi sonuçlarına göre, % 0,2 CHX solüsyonunun zamanla hücre canlılığını azalttığı görüldü (p<0,0001). LDH testi sonuçlarına göre ise % 0,2 CHX’in sitotoksik etkinliğinin kontrol grubuna kıyasla arttığı (p<0,0001) ancak zaman içinde istatistiksel olarak anlamlı bir fark göstermediği (p>0,05) gözlendi. Sonuç: Bu in vitro çalışmanın sınırları dahilinde, % 0,2 CHX solüsyonu, 30 sn ve 2 dk’lık kısa maruz kalma sürelerinde, HGF hücre canlılığını azalttı ve HGF üzerinde sitotoksik etki gösterdi.Publication Open Access Effect of different immobilization media on breakdown of whey proteins by Streptococcus thermophilus(2022-05-01) YILMAZ GÖLER, AYŞE MİNE; Safak F. Z., Bıcım G., Yılmaz A. M., Aksu M. B., Yalcın A. S.Objective: In this study, we aimed to compare the efficiency of different immobilization media to facilitate breakdown of whey proteins by Streptococcus thermophilus (S. thermophilus). Materials and Methods: S. thermophilus was isolated from yoghurt. High-protein whey powder was present in fermentation media and two-phase dispersion technique was used for immobilization of S. thermophilus in agar, agarose and κ-carrageenan. Total protein after fermentation of whey proteins with S. thermophilus in different media was measured. We have also performed sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis to observe changes in individual whey proteins after fermentation in different media. Results:Total protein concentration showed a significant decrease at the end of 24 hours of fermentation in all media. SDS-PAGE results showed that the amount of both α-lactalbumin and β-lactoglobulin were reduced in all immobilization media compared to control. The effect of κ-carrageenan was considerably higher compared to other media. Conclusion:Our results showed that immobilization in κ-carrageenan increased the breakdown of whey proteins by S. thermophilus and can be used to increase fermentation efficiencyPublication Metadata only Primary adrenal insufficiency in a patient with biallelic QRSL1 mutations(2022-09-01) YILMAZ GÖLER, AYŞE MİNE; GÜRAN, TÜLAY; Dursun F., Genc H. M. , YILMAZ GÖLER A. M. , Tas I., Eser M., Pehlivanoglu C., Yilmaz B. K. , GÜRAN T.Publication Metadata only Lakozamid ile birlikte karbamazepin, fenobarbital veya valproik asit kullanımının hepatik metabolizma üzerindeki in vitro etkileri(2021-11-06) YILMAZ GÖLER, AYŞE MİNE; GÜLHAN, REZZAN; GÜLÇEBİ İDRİZ OĞLU, MEDİNE; YILMAZ GÖLER A. M., ALTINOLUK T., ÇULPAN Y., Selalmaz Y., ONAT F., GÜLHAN R., GÜLÇEBİ İDRİZ OĞLU M.Amaç: Karbamazepin ve fenobarbital hepatik sitokromP450 (CYP) metabolizma enzimlerini indükleyerek, valproik asit ise inhibe ederek ilaçilaç etkileşimlerine yol açabilmektedir. Yeni jenerasyon anti-nöbet ilaçlardan (ASD) biri olan lakozamid (LCM), CYP2C19, CYP2C9 ve CYP3A4 enzimleri ile metabolizma olmaktadır. Çalışmamızda lakozamid ile birlikte kullanılan ASD’lerin CYP enzimleri üzerindeki etkilerini in vitro yöntemlerle mRNA düzeyinde araştırmayı amaçladık. Gereç ve Yöntem: İnsan hepatoselüler karsinom hücrelerine (HepaRG veya HepG2) terapötik konsantrasyonlarda karbamazepin, fenobarbital veya valproik asit uygulanmasının ardından, kuyucuklara kanda minimum veya maksimum terapötik konsantrasyonlara (Cmin veya Cmaks) karşılık gelecek şekilde LCM eklendi. Hücre örneklerinde CYP2C19, CYP2C9 ve CYP3A4 genlerinin mRNA ekspresyon düzeyleri gerçek zamanlı polimeraz zincir reaksiyonu ile ölçüldü ve değişiklikler kat değişimi (FC) değerleri olarak hesaplandı. FC değerleri 0,5’in altında kalan gen ekspresyonları inhibisyon, 2’nin üzerinde olanlar ise indüksiyon olarak değerlendirildi ve GraphPad Prism 8 ile analiz edildi. Bulgular: HepaRG hücrelerinde en yüksek mRNA ekspresyonu CYP2C19 ve CYP3A4 genlerinde karbamazepin ve LCMCmin'nin birlikte uygulandığı gruplarda (sırasıyla, FC:5,2; FC:1009,9) saptanırken, CYP2C9 gen ekspresyonu, fenobarbital ile LCMCmin uygulanan grupta anlamlı derecede yüksek bulundu (FC:795,16). HepaRG hücrelerinde valproik asit ile LCMCmaks uygulanan grupta en düşük CYP gen ekspresyonu CYP3A4 olarak tespit edildi (FC:2,3). HepG2 hücrelerinde ise CYP genlerinin ekspresyonları anlamlı değişiklik göstermedi. Sonuç: CYP enzim ekspresyonunun daha yüksek oranda gerçekleştiği HepaRG hücre hattı deneylerinden elde ettiğimiz sonuçlar özellikle karbamazepin ve fenobarbitalin CYP genlerinin ekspresyonlarını değiştirerek (sırasıyla CYP2C19-CYP3A4 ve CYP2C9) LCM ile etkileşmeye girebileceğine işaret etmektedir. Sonuçlarımızın doğrulanması için LCM konsantrasyonunun eş zamanlı olarak ölçülmesi ve deney sayılarının arttırılması planlanmaktadır. Çalışmamız Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) (119R041) tarafından desteklenmektedir.