Publication: Klorheksidinin insan dişeti fibroblastları üzerindeki hücre canlılığı ve sitotoksisite etkinliğinin in vitro koşullarda değerlendirilmesi
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Date
2023-04-01
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Abstract
Amaç: Bu in vitro çalışmada, % 0,2’lik klorheksidin (CHX)
solüsyonunun, insan dişeti fibroblast (HGF) hücre canlılığı
ve sitotoksisitesi üzerindeki etkilerinin değerlendirilmesi
amaçlanmıştır.
Gereç ve Yöntemler: Bu çalışma 30 saniye ve 2 dakikalık
zaman aralıklarında, nötr pH değerindeki % 0,2’lik CHX solüsyonu
ve hücre olarak ATCC’den ticari olarak temin edilen HGF-1 (CRL2014) hücre hatları kullanılarak gerçekleştirildi. CHX’in HGF
üzerindeki hücre canlılığı etkileri 3-(4,5-dimetiltiazol-2-il)-2,5-
difeniltetrazolyum bromür (MTT) testi ile ve sitotoksik etkileri
laktat dehidrogenaz (LDH) testi ile değerlendirildi. Sonuçlar
ortalama ve standart sapma değerleri kullanılarak two way ANOVA
testiyle istatistiksel olarak analiz edildi (p<0,05).
Bulgular: MTT testi sonuçlarına göre, % 0,2 CHX solüsyonunun
zamanla hücre canlılığını azalttığı görüldü (p<0,0001). LDH testi
sonuçlarına göre ise % 0,2 CHX’in sitotoksik etkinliğinin kontrol
grubuna kıyasla arttığı (p<0,0001) ancak zaman içinde istatistiksel
olarak anlamlı bir fark göstermediği (p>0,05) gözlendi.
Sonuç: Bu in vitro çalışmanın sınırları dahilinde, % 0,2 CHX
solüsyonu, 30 sn ve 2 dk’lık kısa maruz kalma sürelerinde, HGF
hücre canlılığını azalttı ve HGF üzerinde sitotoksik etki gösterdi.
Objectives: The aim of this study was to evaluate the effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts’ (HGF) cell viability and cytotoxicity in vitro. Materials and Methods: This study was performed at 30-second and 2-minute time intervals using a 0.2 % CHX solution at neutral pH and HGF-1 (CRL-2014) cell lines commercially available from ATCC. Cell viability effects of CHX on HGF were determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) test, and cytotoxic effects were determined using the lactate dehydrogenase (LDH) test. Results were statistically analyzed by calculating means and standard deviations using the two-way ANOVA test (p<0.05). Results: According to the MTT test results, it was observed that the 0.2 % CHX solution decreased cell viability over time (p<0.0001). According to the LDH test results, the cytotoxic efficacy of 0.2 % CHX was higher compared to the control group (p<0.0001), but did not show a statistically significant difference over time (p>0.05). Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.
Objectives: The aim of this study was to evaluate the effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts’ (HGF) cell viability and cytotoxicity in vitro. Materials and Methods: This study was performed at 30-second and 2-minute time intervals using a 0.2 % CHX solution at neutral pH and HGF-1 (CRL-2014) cell lines commercially available from ATCC. Cell viability effects of CHX on HGF were determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5- diphenyltetrazolium bromide (MTT) test, and cytotoxic effects were determined using the lactate dehydrogenase (LDH) test. Results were statistically analyzed by calculating means and standard deviations using the two-way ANOVA test (p<0.05). Results: According to the MTT test results, it was observed that the 0.2 % CHX solution decreased cell viability over time (p<0.0001). According to the LDH test results, the cytotoxic efficacy of 0.2 % CHX was higher compared to the control group (p<0.0001), but did not show a statistically significant difference over time (p>0.05). Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.
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Keywords
klorheksidin, fibroblast, in vitro, sitotoksisite, hücre canlılık testleri, chlorhexidine, fibroblasts, cytotoxicity, cell viability assays
Citation
Bayraktar G., YILMAZ GÖLER A. M., ÖZTÜRK H., "Klorheksidinin İnsan Dişeti Fibroblastları Üzerindeki Hücre Canlılığı ve Sitotoksisite Etkinliğinin İn vitro Koşullarda Değerlendirilmesi", European Journal of Research in Dentistry, cilt.7, sa.1, ss.27-32, 2023