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Synthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute

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dc.contributor.authorSEZER, ALİ DEMİR
dc.contributor.authorUĞURLU, TİMUÇİN
dc.contributor.authorŞAHBAZ, SEVİNÇ
dc.contributor.authorsDoğan M., Şahbaz S., Uğurlu T., Sezer A. D.
dc.date.accessioned2023-08-31T08:20:22Z
dc.date.available2023-08-31T08:20:22Z
dc.date.issued2022-08-01
dc.description.abstractThe aim of the current study was to assess the physicochemical characteristics and wound healing activity of chitosan-polyvinyl alcohol (PVA) crosslinked hydrogel containing recombinant human epidermal growth factor (rh-EGF) or recombinant mouse epidermal growth factor (rm-EGF). The hydrogels were prepared and analyses were made of the morphological properties, viscosity, water absorption capacity, mechanical and bio-adhesive properties. The viscosity of the formulations varied between 14.400 - 48.500 cPs, with the greatest viscosity values determined in K2 formulation. F2 formulation showed the highest water absorption capacity. According to the studies of the mechanical properties, H2 formulation (0.153±0.018 N.mm) showed the greatest adhesiveness and E2 (0.245±0.001 mj/cm2 ) formulation, the highest bio-adhesion values. Hydrogels were cytocompatible considering in vitro cell viability values of over 76% on human keratinocyte cells (HaCaT, CVCL-0038) and of over 84% on human fibroblast cells (NIH 3T3, CRL-1658) used as a model cell line. According to the BrdU cell proliferation results, B1 (197.82±2.48%) formulation showed the greatest NIH 3T3 and C1 (167.43±5.89%) formulation exhibited the highest HaCaT cell proliferation ability. In addition, the scratch closure assay was performed to assess the wound healing efficiency of formulation and the results obtained in the study showed that F2 formulation including PEGylated rh-EGF had a highly effective role. Keywords: Chitosan. rh-EGF. Hydrogel. Texture analyzer. Cell culture.
dc.identifier.citationDoğan M., Şahbaz S., Uğurlu T., Sezer A. D., "Synthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute", BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES, cilt.58, ss.1-19, 2022
dc.identifier.doi10.1590/s2175-97902022e191120s
dc.identifier.endpage19
dc.identifier.issn1984-8250
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/11424/293021
dc.identifier.volume58
dc.language.isoeng
dc.relation.ispartofBRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSağlık Bilimleri
dc.subjectEczacılık
dc.subjectEczacılık Teknolojisi
dc.subjectHealth Sciences
dc.subjectPharmacology and Therapeutics
dc.subjectPharmaceutics Technology
dc.subjectKlinik Tıp (MED)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectFarmakoloji ve Toksikoloji
dc.subjectFARMAKOLOJİ VE ECZACILIK
dc.subjectClinical Medicine (MED)
dc.subjectLife Sciences (LIFE)
dc.subjectPHARMACOLOGY & TOXICOLOGY
dc.subjectPHARMACOLOGY & PHARMACY
dc.subjectFarmakoloji
dc.subjectFarmakoloji, Toksikoloji ve Eczacılık (çeşitli)
dc.subjectGenel Farmakoloji, Toksikoloji ve Eczacılık
dc.subjectFarmakoloji (tıbbi)
dc.subjectİlaç Rehberleri
dc.subjectYaşam Bilimleri
dc.subjectPharmacy
dc.subjectPharmacology
dc.subjectPharmacology, Toxicology and Pharmaceutics (miscellaneous)
dc.subjectGeneral Pharmacology, Toxicology and Pharmaceutics
dc.subjectPharmacology (medical)
dc.subjectDrug Guides
dc.subjectLife Sciences
dc.subjectChitosan. rh-EGF. Hydrogel. Texture analyzer. Cell culture
dc.titleSynthesis and characterization of chitosan-PVA hydrogel containing PEGylated recombinant epidermal growth factor on cell culture for wound healing substitute
dc.typearticle
dspace.entity.typePublication
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