Publication: Heterologous expression and initial in silico characterization of a novel snakin-Z peptide
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Date
2023-08-01
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Abstract
Heterologous expression of the plant-derived snakin-Z (SNK-Z) peptide was carried out inEscherichia coliBL21 and Mach 1 strains using the pET14b, and pGEX-6P-1 vectors, and its tertiary structure was determined. The most efficient production of the recombinant fusion peptide (GST/SNK-Z) in both strains was achieved by induction with 0.1 mM isopropyl β-D-1-thiogalactopyranoside at 32°C. GST/SNK-Z with 30.14kDa which yielded 6.5mg/L protein, was purified by affinity chromatography followed by dialysis. The GST tags were removed using PreScission protease. The IC50of GST/SNK-Z was calculated as 12.07 µM forStaphylococcus aureus(ATCC 25923) using nonlinear regression analysis. The secondary structure of recombinant SNK-Z consisted of α-helix and coil sites. It’s 3-D model was generated with a confidence score of -0.20 and a template modeling score of 0.69 ± 0.12. The predicted solvent accessibility and B-factor profile were detected.This is the first report of heterologous expression of SNK-Z inE. coli. Our study provides fundamental data for the large-scale production of SNK-Z, which has a high potential for use in the pharmaceutical industry because of its previously reported antimicrobial effects.
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Yaşam Bilimleri, Biyoteknoloji, Moleküler Biyoloji ve Genetik, Temel Bilimler, Life Sciences, Biotechnology, Molecular Biology and Genetics, Natural Sciences, Temel Bilimler (SCI), Yaşam Bilimleri (LIFE), Doğa Bilimleri Genel, Mikrobiyoloji, ÇOK DİSİPLİNLİ BİLİMLER, BİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ, Natural Sciences (SCI), Life Sciences (LIFE), NATURAL SCIENCES, GENERAL, MOLECULAR BIOLOGY & GENETICS, MICROBIOLOGY, MULTIDISCIPLINARY SCIENCES, BIOTECHNOLOGY & APPLIED MICROBIOLOGY, Uygulamalı Mikrobiyoloji ve Biyoteknoloji, Moleküler Tıp, Moleküler Biyoloji, Multidisipliner, Applied Microbiology and Biotechnology, Molecular Medicine, Molecular Biology, Multidisciplinary
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Teker T., Albayrak G., Turan K., "Heterologous Expression and Initial In Silico Characterization of a Novel Snakin-Z Peptide", INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, cilt.29, sa.5, ss.1-11, 2023