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Heterologous expression and initial in silico characterization of a novel snakin-Z peptide

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dc.contributor.authorTURAN, KADİR
dc.contributor.authorsTeker T., Albayrak G., Turan K.
dc.date.accessioned2023-08-09T12:20:34Z
dc.date.available2023-08-09T12:20:34Z
dc.date.issued2023-08-01
dc.description.abstractHeterologous expression of the plant-derived snakin-Z (SNK-Z) peptide was carried out inEscherichia coliBL21 and Mach 1 strains using the pET14b, and pGEX-6P-1 vectors, and its tertiary structure was determined. The most efficient production of the recombinant fusion peptide (GST/SNK-Z) in both strains was achieved by induction with 0.1 mM isopropyl β-D-1-thiogalactopyranoside at 32°C. GST/SNK-Z with 30.14kDa which yielded 6.5mg/L protein, was purified by affinity chromatography followed by dialysis. The GST tags were removed using PreScission protease. The IC50of GST/SNK-Z was calculated as 12.07 µM forStaphylococcus aureus(ATCC 25923) using nonlinear regression analysis. The secondary structure of recombinant SNK-Z consisted of α-helix and coil sites. It’s 3-D model was generated with a confidence score of -0.20 and a template modeling score of 0.69 ± 0.12. The predicted solvent accessibility and B-factor profile were detected.This is the first report of heterologous expression of SNK-Z inE. coli. Our study provides fundamental data for the large-scale production of SNK-Z, which has a high potential for use in the pharmaceutical industry because of its previously reported antimicrobial effects.
dc.identifier.citationTeker T., Albayrak G., Turan K., "Heterologous Expression and Initial In Silico Characterization of a Novel Snakin-Z Peptide", INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, cilt.29, sa.5, ss.1-11, 2023
dc.identifier.doi10.1007/s10989-023-10556-9
dc.identifier.endpage11
dc.identifier.issn1573-3149
dc.identifier.issue5
dc.identifier.startpage1
dc.identifier.urihttps://link.springer.com/article/10.1007/s10989-023-10556-9
dc.identifier.urihttps://hdl.handle.net/11424/292199
dc.identifier.volume29
dc.language.isoeng
dc.relation.ispartofINTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectYaşam Bilimleri
dc.subjectBiyoteknoloji
dc.subjectMoleküler Biyoloji ve Genetik
dc.subjectTemel Bilimler
dc.subjectLife Sciences
dc.subjectBiotechnology
dc.subjectMolecular Biology and Genetics
dc.subjectNatural Sciences
dc.subjectTemel Bilimler (SCI)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectDoğa Bilimleri Genel
dc.subjectMikrobiyoloji
dc.subjectÇOK DİSİPLİNLİ BİLİMLER
dc.subjectBİYOTEKNOLOJİ VE UYGULAMALI MİKROBİYOLOJİ
dc.subjectNatural Sciences (SCI)
dc.subjectLife Sciences (LIFE)
dc.subjectNATURAL SCIENCES, GENERAL
dc.subjectMOLECULAR BIOLOGY & GENETICS
dc.subjectMICROBIOLOGY
dc.subjectMULTIDISCIPLINARY SCIENCES
dc.subjectBIOTECHNOLOGY & APPLIED MICROBIOLOGY
dc.subjectUygulamalı Mikrobiyoloji ve Biyoteknoloji
dc.subjectMoleküler Tıp
dc.subjectMoleküler Biyoloji
dc.subjectMultidisipliner
dc.subjectApplied Microbiology and Biotechnology
dc.subjectMolecular Medicine
dc.subjectMolecular Biology
dc.subjectMultidisciplinary
dc.titleHeterologous expression and initial in silico characterization of a novel snakin-Z peptide
dc.typearticle
dspace.entity.typePublication
local.avesis.id750b6e7f-4da3-4341-b4d4-568528c0e148
relation.isAuthorOfPublication9cc23fc2-5031-46cd-91a0-a2e13ab788d4
relation.isAuthorOfPublication.latestForDiscovery9cc23fc2-5031-46cd-91a0-a2e13ab788d4

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