Publication: Preparation of a Poly(ethylene glycol)-Based Cross-Linked Network from a Click Reaction for Enzyme Immobilization
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Date
2019
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WILEY-V C H VERLAG GMBH
Abstract
In this study, a polyethylene glycol (PEG)-maleimide film was prepared by a thiol-ene click reaction. The covalent immobilization of amylase was carried out onto the PEG-maleimide based photo-curable network via epoxy and C=O groups without any linker. The epoxy and carbonyl groups on the PEG backbone improved the affinity of the enzyme. The immobilization yield of amylase onto the film was 72.46%. The immobilization capacity was also determined as 313 mg/g. The thermal stability of amylase improved via covalent attachment. Immobilized amylase showed maximum activity at 40 degrees C and pH 6.5. The immobilized amylase continued to retain 66% of its initial activity after 30 days.
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Keywords
amylase, click chemistry, enzyme immobilization, thiol-ene, ALPHA-AMYLASE, SUPPORTS, GLYCOL, PEG, FUNCTIONALIZATION, OPTIMIZATION, CONJUGATION, MALEIMIDES, STABILITY, KINETICS