Publication: Preparation of a Poly(ethylene glycol)-Based Cross-Linked Network from a Click Reaction for Enzyme Immobilization
| dc.contributor.author | OKTAY, BURCU | |
| dc.contributor.authors | Oktay, Burcu; Demir, Serap; Kayaman-Apohan, Nilhan | |
| dc.date.accessioned | 2022-03-12T22:28:54Z | |
| dc.date.available | 2022-03-12T22:28:54Z | |
| dc.date.issued | 2019 | |
| dc.description.abstract | In this study, a polyethylene glycol (PEG)-maleimide film was prepared by a thiol-ene click reaction. The covalent immobilization of amylase was carried out onto the PEG-maleimide based photo-curable network via epoxy and C=O groups without any linker. The epoxy and carbonyl groups on the PEG backbone improved the affinity of the enzyme. The immobilization yield of amylase onto the film was 72.46%. The immobilization capacity was also determined as 313 mg/g. The thermal stability of amylase improved via covalent attachment. Immobilized amylase showed maximum activity at 40 degrees C and pH 6.5. The immobilized amylase continued to retain 66% of its initial activity after 30 days. | |
| dc.identifier.doi | 10.1002/slct.201900296 | |
| dc.identifier.issn | 2365-6549 | |
| dc.identifier.uri | https://hdl.handle.net/11424/235337 | |
| dc.identifier.wos | WOS:000473422500008 | |
| dc.language.iso | eng | |
| dc.publisher | WILEY-V C H VERLAG GMBH | |
| dc.relation.ispartof | CHEMISTRYSELECT | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | amylase | |
| dc.subject | click chemistry | |
| dc.subject | enzyme immobilization | |
| dc.subject | thiol-ene | |
| dc.subject | ALPHA-AMYLASE | |
| dc.subject | SUPPORTS | |
| dc.subject | GLYCOL | |
| dc.subject | PEG | |
| dc.subject | FUNCTIONALIZATION | |
| dc.subject | OPTIMIZATION | |
| dc.subject | CONJUGATION | |
| dc.subject | MALEIMIDES | |
| dc.subject | STABILITY | |
| dc.subject | KINETICS | |
| dc.title | Preparation of a Poly(ethylene glycol)-Based Cross-Linked Network from a Click Reaction for Enzyme Immobilization | |
| dc.type | article | |
| dspace.entity.type | Publication | |
| local.avesis.id | 3ca54a6c-b7c3-4ddf-939e-9ced2e3a7f49 | |
| local.import.package | SS17 | |
| local.indexed.at | WOS | |
| local.indexed.at | SCOPUS | |
| local.journal.numberofpages | 5 | |
| local.journal.quartile | Q3 | |
| oaire.citation.endPage | 6059 | |
| oaire.citation.issue | 20 | |
| oaire.citation.startPage | 6055 | |
| oaire.citation.title | CHEMISTRYSELECT | |
| oaire.citation.volume | 4 | |
| relation.isAuthorOfPublication | 73bf1f8b-c472-46a3-8ef3-f25a516fef62 | |
| relation.isAuthorOfPublication.latestForDiscovery | 73bf1f8b-c472-46a3-8ef3-f25a516fef62 |