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Hepatosellüler karsinomada glut1 inhibitörü bay-876’nın hepg2 hücrelerinde ve steatoz hücre modelinde glukoz alımı ve hücre çoğalmasına etkisi

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dc.contributor.authorAYDIN OMAY, BANU
dc.contributor.authorCABADAK, HÜLYA
dc.contributor.authorsTanriverdi Bademci A. M., Aydın Omay B., Cabadak H.
dc.date.accessioned2022-12-28T08:36:08Z
dc.date.available2022-12-28T08:36:08Z
dc.date.issued2022-12-09
dc.description.abstractHepatocellular carcinoma is one of the deadly cancers with a significant incidence worldwide. In cancer cells, aerobic glycolysis increases with the Warburg effect. Glucose transport across the cell membrane becomes important at this stage. It has been found that there is an increase in GLUT1 expression in various cancers. BAY-876 is a new generation GLUT1 inhibitor. The affinity of BAY-876 for GLUT1 was found to be approximately 100 times higher than for other glucose transporters. HepG2 cells will be used in molecular analyzes to investigate the role of this novel GLUT1 inhibitor in GLUT1-mediated cell proliferation and glucose uptake into the cell.The effect of BAY-876 on glucose uptake was investigated in the case of bioenergetic stress by applying metformin, an insulin-sensitizing agent with strong antihyperglycemic properties, and in hypoxic environment by applying CoCl2 treatment. A steatosis cell model was created using oleic acid and palmitic acid. We aimed to investigate the effects of BAY-876 on cell proliferation and glucose uptake in HepG2 cell line and HepG2 steatosis cell model. Drugs were added to the culture medium. its effect on cell proliferation was investigated with the BrDU method. Cell proliferation, glucose uptake and colony formation were investigated in HepG2 cell line and HepG2 steatosis cell model in the presence and absence of BAY-876. BAY-876 inhibits cell proliferation and reduces glucose uptake in HepG2 cells and steatosis cell model. BAY-876 was also found to reduce glucose uptake in normal and steatosis cell models under bioenergetic and hypoxic stress conditions. It is predicted that our study will contribute to the literature, provide a different perspective to anticancer drug research studies of GLUT1 inhibitor and contribute to the elucidation of the signal transmission mechanism of hepatocellular carcinoma.
dc.identifier.citationTanriverdi Bademci A. M., Aydın Omay B., Cabadak H., \"HEPATOSELLÜLER KARSİNOMADA GLUT1 İNHİBİTÖRÜ BAY-876’NIN HEPG2 HÜCRELERİNDE VE STEATOZ HÜCRE MODELİNDE GLUKOZ ALIMI VE HÜCRE ÇOĞALMASINA ETKİSİ\", 9. ULUSLARARASI MARMARA FEN BİLİMLERİ KONGRESİ, Kocaeli, Türkiye, 09 Aralık 2022
dc.identifier.startpage50
dc.identifier.urihttps://www.imascon.com/kayit/kayittr/dosyalar/imascon_2022_autumn/imascon_2022_autumn_fen_tammetin_bildiriler.pdf
dc.identifier.urihttps://hdl.handle.net/11424/284453
dc.language.isoeng
dc.relation.ispartof9. ULUSLARARASI MARMARA FEN BİLİMLERİ KONGRESİ
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTıp
dc.subjectSağlık Bilimleri
dc.subjectTemel Tıp Bilimleri
dc.subjectBiyofizik
dc.subjectMedicine
dc.subjectHealth Sciences
dc.subjectFundamental Medical Sciences
dc.subjectBiophysics
dc.subjectKlinik Tıp (MED)
dc.subjectYaşam Bilimleri (LIFE)
dc.subjectKlinik Tıp
dc.subjectBiyoloji ve Biyokimya
dc.subjectTIP, GENEL & DAHİLİ
dc.subjectBİYOFİZİK
dc.subjectClinical Medicine (MED)
dc.subjectLife Sciences (LIFE)
dc.subjectCLINICAL MEDICINE
dc.subjectBIOLOGY & BIOCHEMISTRY
dc.subjectMEDICINE, GENERAL & INTERNAL
dc.subjectBIOPHYSICS
dc.subjectGenel Sağlık Meslekleri
dc.subjectPatofizyoloji
dc.subjectTemel Bilgi ve Beceriler
dc.subjectDeğerlendirme ve Teşhis
dc.subjectDahiliye
dc.subjectAile Sağlığı
dc.subjectTıp (çeşitli)
dc.subjectGenel Tıp
dc.subjectYaşam Bilimleri
dc.subjectGeneral Health Professions
dc.subjectPathophysiology
dc.subjectFundamentals and Skills
dc.subjectAssessment and Diagnosis
dc.subjectInternal Medicine
dc.subjectFamily Practice
dc.subjectMedicine (miscellaneous)
dc.subjectGeneral Medicine
dc.subjectLife Sciences
dc.subjectHepatocellular carcinoma
dc.subjectBAY-876
dc.subjectsteatosis
dc.subjectmetformin
dc.titleHepatosellüler karsinomada glut1 inhibitörü bay-876’nın hepg2 hücrelerinde ve steatoz hücre modelinde glukoz alımı ve hücre çoğalmasına etkisi
dc.typeconferenceObject
dspace.entity.typePublication
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