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Browsing Akademik Arşiv / Academic Archive by Publisher "ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY"
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Publication Metadata only An EST-SSR marker linked with yellow rust resistance in wheat(ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY, 2010) AYDİN, YILDIZ; Ercan, S.; Ertugrul, F.; Aydin, Y.; Akfirat, F. S.; Hasancebi, S.; Akan, K.; Mert, Z.; Bolat, N.; Yorgancilar, O.; Altinkut-Uncuoglu, A.Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F-2 seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F-2 hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.Publication Metadata only High frequency somatic embryogenesis in cotton(ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY, 2004) Aydin, Y; Ipekci, Z; Talas-Ogras, T; Zehir, A; Bajrovic, K; Gozukirmizi, NA highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton ( Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100%) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm(-3) polyvinylpyrrolidone (PVP), 1 mg dm(-3) 6-benzylaminopurine (BAP), 0.5 mg dm(-3) kinetin for Nazilli M-503 and 1 g dm(-3) PVP, 2 mg dm(-3) BAP, 0.5 mg dm(-3) kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm(-3) PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2% from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9%) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 - 30%.