Publication: An EST-SSR marker linked with yellow rust resistance in wheat
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Date
2010
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ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY
Abstract
Expressed sequenced tags containing simple sequence repeats (EST-SSRs) were used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.). A cross between yellow rust resistant (PI178383) and susceptible (Harmankaya99) wheat genotypes was performed and respective DNA pools from the resistant and susceptible F-2 seedlings were constructed. 78 EST-SSR primers were used for bulked segregant analysis and one EST-SSR marker (Pk54), identified as 200 bp fragment, was present in the resistant parent and resistant F-2 hybrids but not in the susceptible ones. 108 wheat genotypes differing in yellow rust resistance were screened with Pk54 and 68 % of the wheat genotypes, known to be yellow rust resistant, had the Pk54 marker, further suggesting that the presence of this marker correlates with yellow rust resistance.
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Keywords
breeding, Puccinia striiformis f. sp tritici, Triticum aestivum, MICROSATELLITE MARKERS, DISEASE RESISTANCE, GENETIC DIVERSITY, STRIPE RUST, IDENTIFICATION, BARLEY, SEQUENCES, RICE, TRANSFERABILITY, POLYMORPHISMS