Person: ŞEN, ALİ
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ŞEN
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ALİ
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Publication Metadata only Morphological and biochemical evaluation of effects of myrtus communis l. Extract on heart and aorta in high fat-diet induced obese rats(2023-05-01) ŞEN, ALİ; ERCAN, FERİHA; Özyılmaz Yay N., Bülbül Aycı N., Kaya R., Şen A., Şener G., Ercan F.Objectives: The purpose of this study was to examine the protective effects of Myrtus communis L. (MC) extract on high fat-diet (HFD) induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide system (eNOS). Materials and Methods: Wistar albino male rats were divided into 3 groups (n=7) as control, HFD and HFD+MC. Rats in HFD and HFD+MC groups were HFD fed for 16 weeks and in the last 4 weeks saline or MC (100 mg/kg) was administered orally (5 days/week). Triglyceride, cholesterol and HDL were estimated in blood serum. Tissue oxidative stress and inflammatory parameters were evaluated biochemically. Tissues morphologies, eNOS, inducible NOS (iNOS) and NADPH oxidase-2 (NOX-2)-immunopositive and apoptotic cells were evaluated histologically. Results: Altered serum lipid profiles, degenerated heart and aorta morphology, increased malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, tumor necrosis factor-alpha, monocyte chemoattractant protein-1 and myeloperoxidase levels and iNOS, NOX-2 immunopositive and apoptotic cells, decreased NO levels, eNOS-immunopositive cells in both tissues were observed in HFD group. All these parameters improved in HFD+MC group. Conclusion: This study revealed that HFD-induced obesity increased iNOS activation and oxidative stress in both tissues. MC regulated oxidant/antioxidant had balanced thus preventing heart and aorta damage via eNOS involvement.Publication Metadata only THERAPEUTIC POTENTIAL OF MYRTUS COMMUNIS SUBSP COMMUNIS EXTRACT AGAINST ACETIC ACID-INDUCED COLONIC INFLAMMATION IN RATS(WILEY, 2017) ŞEN, ALİ; Sen, Ali; Yuksel, Meral; Bulut, Gizem; Bitis, Leyla; Ercan, Feriha; Ozyilmaz-Yay, Nagehan; Akbulut, Ozben; Cobanoglu, Hamit; Ozkan, Sevil; Sener, GokselThe aim of this study was to evaluate the effect of ethanol extract from leaves of Myrtus communis subsp. communis (MC) on acetic acid (AA)-induced ulcerative colitis in rats. On the fourth day of colitis induction, all rats were decapitated. Colitis was assessed by macroscopic and microscopic scores and by measuring malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity, luminol, lucigenin, nitric oxid and peroxynitrite chemiluminescence (CL). Colitis caused significant increases in the colonic MDA levels, MPO activity, CL values, macroscopic and microscopic damage scores along with significant decrease in tissue GSH level. However, treatment with MC extract reversed all these biochemical indices, as well as histopathological alterations induced by AA with the protective effects being similar to that of sulphasalazine treatment. The study showed that MC extract could alleviate colitis in rats and can be considered an alternative therapeutic approach for management of inflammatory bowel diseases (IBD).Publication Metadata only Effects of Myrtus communis extract treatment in bile duct ligated rats(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2016) ŞEN, ALİ; Sen, Ali; Ozkan, Sevil; Recebova, Kafiye; Cevik, Ozge; Ercan, Feriha; Demirci, Elif Kervancioglu; Bitis, Leyla; Sener, GokselBackground: The aim of our study was to investigate the antifibrotic and antioxidant effects of Myrtus communis subsp. communis (MC) extract against liver injury and fibrosis occurring in rats with biliary obstruction. Materials and methods: The rats were randomized into four groups (n = 8). Control group (C), MC-administrated group (MC), the bile duct ligation (BDL), and BDL + MC groups. MC was administered at a dose of 50 mg/kg a day orally for 28 days. In blood samples, total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase levels, tumor necrosis factor-alpha, and interleukin-1 beta measurement were measured. Oxidative injury was examined by measuring luminol and lucigenin chemiluminescence, malondialdehyde and glutathione levels, superoxide dismutase and myeloperoxidase activities. Transforming growth factor-beta and hydroxyproline levels were measured for analyzing fibrosis. The hepatic injury was also analyzed microscopically. Results: Plasma total bilirubin, direct bilirubin, alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor-alpha, and interleukin-1 beta levels were found significantly high in the BDL group, while these values significantly decreased in the BDL group treated with MC. On the other hand, the glutathione and superoxide dismutase values significantly decreased in the BDL group compared to the control group but increased markedly in BDL + MC group compared to the BDL group. Malondialdehyde levels, myeloperoxidase activity, tissue luminol, lucigenin, transforming growth factor-beta, and hydroxyproline levels when compared with the control group increased dramatically in the BDL group and reduced the MC + BDL group. Conclusions: Our results suggest that MC protects the liver tissues against oxidative damage following BDL via its radical scavenging and antioxidant activities, which appear to involve the inhibition of tissue neutrophil infiltration. (C) 2016 Elsevier Inc. All rights reserved.