Person: ŞEN, ALİ
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ŞEN
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ALİ
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Publication Metadata only Morphological and biochemical evaluation of effects of myrtus communis l. Extract on heart and aorta in high fat-diet induced obese rats(2023-05-01) ŞEN, ALİ; ERCAN, FERİHA; Özyılmaz Yay N., Bülbül Aycı N., Kaya R., Şen A., Şener G., Ercan F.Objectives: The purpose of this study was to examine the protective effects of Myrtus communis L. (MC) extract on high fat-diet (HFD) induced heart and aorta damage by evaluating oxidative stress and the endothelial nitric oxide system (eNOS). Materials and Methods: Wistar albino male rats were divided into 3 groups (n=7) as control, HFD and HFD+MC. Rats in HFD and HFD+MC groups were HFD fed for 16 weeks and in the last 4 weeks saline or MC (100 mg/kg) was administered orally (5 days/week). Triglyceride, cholesterol and HDL were estimated in blood serum. Tissue oxidative stress and inflammatory parameters were evaluated biochemically. Tissues morphologies, eNOS, inducible NOS (iNOS) and NADPH oxidase-2 (NOX-2)-immunopositive and apoptotic cells were evaluated histologically. Results: Altered serum lipid profiles, degenerated heart and aorta morphology, increased malondialdehyde, 8‐hydroxy‐2‐deoxyguanosine, tumor necrosis factor-alpha, monocyte chemoattractant protein-1 and myeloperoxidase levels and iNOS, NOX-2 immunopositive and apoptotic cells, decreased NO levels, eNOS-immunopositive cells in both tissues were observed in HFD group. All these parameters improved in HFD+MC group. Conclusion: This study revealed that HFD-induced obesity increased iNOS activation and oxidative stress in both tissues. MC regulated oxidant/antioxidant had balanced thus preventing heart and aorta damage via eNOS involvement.Publication Metadata only Determination of anti-cancer properties of urtica dioica ethanol extract on hematopoietic cell lines(2022-09-01) MEGA TİBER, PINAR; ŞEN, ALİ; ORUN, OYA; Mega Tiber P., Karadeniz M., Sevinç S. K. , Şen A., Orun O.Aim: Urtica dioica is a natural phytotherapeutic agent with various effects such as antioxidant, anti-inflammatory, anti-fungal, hypotensive as well as anti-carcinogenic. In recent years, phytotherapeutic approaches to reduce side effects of available chemotherapeutic agents or to find new agents with higher toxicity in cancer cells relative to healthy ones, are area of intense research. Therefore, in this study, we investigated anti-carcinogenic effects of U. dioica ethanol extract on K562 human chronic myeloid erythrocyte cell lines and on lymphocytes isolated from healthy donors (PBMC).Material and Methods: U. dioica extract was isolated by using soxhlet extraction methodology. Anti-carcinogenic effects of the agent were analyzed with MTT assay (Cell Proliferation Kit I, Roche), Annexin V/PI (Molecular Probes A10788), mitochondrial membrane potential assay (JC-1, Abnova KA1324) and cell cycle analyses (PI staining in flow cytometer (FACS Calibur). PBMCs were prepared from healthy volunteers through Ficoll Hypaque density gradients.Results: Data indicated that treatment with U. dioica didn’t show up a dose-dependent cytotoxic effect on K562 cell line, but there was a 20% suppression at 25 μg/ml compared to PBMC (p<0.001). U. dioica extract yielded to an increase in apoptosis 13% and 41% for 25 μg/ml and 100 μg/ml, respectively, in comparison to untreated control cells (p=0.033). It has also induced cell cycle arrest at G0/G1 phase, in a dose-dependent manner. Accumulation at G0/G1 phase increased 9.6, 10 and 14% in 50, 75 and 100 μg/ml concentrations, respectively, compared to the control group (p<0.0001).Conclusion: Our findings explicitly show that the total ethanol extract of U. dioica is highly effective in K562 cells on the induction of apoptosis and cell cycle arrest at reasonably low concentrations and these effects significantly pronounced compared to healthy controls.